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Preparation method and ultrafiltration apparatus of monosialic acid tetrahexose ganglioside GM1 preparation

A ganglioside and monosialic acid technology, applied in the field of biochemical pharmaceuticals, can solve problems such as difficult application, complicated separation and purification process, and environmental pollution, so as to eliminate potential safety hazards, improve clinical repair and treatment efficacy, and improve safety effect

Inactive Publication Date: 2014-05-14
吕维学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the description of the patent application for this invention, it can be seen that there are the following deficiencies: first, the separation and purification process is complicated, and many harmful organic solvents are used for enzymatic hydrolysis and addition, which is not only difficult to apply in industry but also causes environmental pollution; The second is that the solvent recovery process uses a membrane with a pore size of 10,000 to 100,000 Daltons, preferably about 50,000 Daltons, for diafiltration. This diafiltration process is a pressure-free natural diafiltration, which is time-consuming and labor-intensive. , and less efficient

Method used

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  • Preparation method and ultrafiltration apparatus of monosialic acid tetrahexose ganglioside GM1 preparation
  • Preparation method and ultrafiltration apparatus of monosialic acid tetrahexose ganglioside GM1 preparation

Examples

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Effect test

Embodiment 1

[0020] The preparation method of the monosialotetrahexosyl ganglioside GM1 preparation of Example 1 of the present invention comprises the following steps:

[0021] (1) Prepare mixed homogenate:

[0022] Collect fresh pig brains from the meat joint factory, remove the broken bones, meninges, skull base blood vessels, etc., clear with distilled water, add 3 times the volume of a mixed solvent of chloroform, methanol, and water after weighing, and the blending ratio is 1:1: 0.5, placed in a high-shear homogeneous emulsification tank, homogenized the raw materials at 6000-8000 rpm for 30 minutes, poured into an 800-mesh stainless steel sieve, filtered, and removed the residue to obtain a lipid mixed homogenate.

[0023] (2) Preparation of GM1 primary extract:

[0024] Send the filtered mixed homogenate into the hanging basket air filter press, and filter through the air compression to remove the residue; take back the filtrate and let it stand for stratification, and collect the...

Embodiment 2

[0031] The preparation method of the monosialotetrahexosyl ganglioside GM1 preparation of Example 2 of the present invention comprises the following steps:

[0032] (1) Prepare mixed homogenate:

[0033] Collect fresh pig brains from the meat joint factory, remove the broken bones, meninges, and blood vessels at the base of the skull, wash them with distilled water, add 5 times the volume of a mixed solvent of chloroform, methanol, and water after weighing, and the blending ratio is 1:1: 0.8, put it in a colloid mill and grind it first, then pour it into a high-shear homogeneous emulsification tank and homogenize it at a speed of 8000 rpm for 60 minutes, then pour it into an 800-mesh stainless steel sieve to filter, remove the residue, Obtain lipid mixed homogenate.

[0034] (2) Preparation of GM1 primary extract:

[0035]Send the lipid mixed homogenate into the hanging basket air filter press, and filter through air compression under the condition of vacuum pressure of -10k...

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Abstract

The invention discloses a preparation method and an ultrafiltration apparatus of a monosialic acid tetrahexose ganglioside GM1 preparation, which belongs to the technical field of biochemistry pharmacy. The preparation method combines a separating technology of ultrafilter membrane and nanofiltration membrane, from the basis that the biological activity is kept and the curative effect of the ganglioside GM1 preparation is increased, the ganglioside GM1 is extracted and purified from pig brain through the technology steps of homogenizing, filter pressing, ultrafiltrating, nanofiltrating and the like, and the purity of ganglioside GM1 can reach more than 95%. According to the invention, relative links of a traditional chromatography and column chromatography purification method can be reduced, harmful organic solvent usage is reduced, so that the preparation method and ultrafiltration apparatus is suitable for whole process industrial production, production cost and production cost are reduced, biology activity can be kept, the curative effect of the ganglioside GM1 preparation is increased, the preparation process is simple, environmentally friendly and pollution free, and the preparation method and ultrafiltration apparatus have outstanding substantive characteristics and obvious improvement.

Description

technical field [0001] The invention relates to the technical field of biochemical pharmacy, in particular to a preparation method of monosialotetrahexosyl ganglioside GM1 preparation and an ultrafiltration device. Background technique [0002] Gangliosides are a class of glycolipids with one or more sialic acid residues, which are widely present on the surface of mammalian brain cell membranes and central nervous tissues. It is known that gangliosides can repair nervous system damage , promote the growth and regeneration of nerves, promote the recovery of innervation function, and protect nerve cell membranes. [0003] Monosialotetrahexosyl ganglioside is one of the main gangliosides, which can be used in many medical applications, especially in the repair and treatment of central and peripheral nervous system disorders, which can promote central Nerve remodeling and functional recovery after nervous system injury have a good curative effect on cerebral and spinal cord tra...

Claims

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Application Information

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IPC IPC(8): C07H15/10C07H1/08B01D61/18
Inventor 吕维学董娜马丽
Owner 吕维学
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