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Detection method with ultraviolet-visible absorption spectrum of animal oil

A technology of absorption spectrum and detection method, which is applied in the field of food inspection and detection, can solve the problems that oil samples cannot be measured, and achieve the effects of easy promotion and use, easy access to materials, and avoidance of cross-contamination

Inactive Publication Date: 2014-05-21
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problems that animal oil must first be dissolved in a solvent when detecting the ultraviolet-visible absorption spectrum in the prior art, and the oil sample cannot be measured when the amount of oil sample is very small, the present invention provides a fast, less required sample amount, and no solvent , A new method for qualitative detection of accurate animal oil UV-visible absorption spectrum

Method used

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  • Detection method with ultraviolet-visible absorption spectrum of animal oil
  • Detection method with ultraviolet-visible absorption spectrum of animal oil
  • Detection method with ultraviolet-visible absorption spectrum of animal oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Adopt the mensuration of polyethylene film method lard ultraviolet-visible absorption spectrum of the present invention:

[0051] (1) Put the double-layer polyethylene film into the optical path of the ultraviolet-visible spectrometer to adjust to zero.

[0052] Due to its simple molecular structure, polyethylene should have a simple UV-visible absorption spectrum. The UV-visible spectrum of its double-layer polyethylene film is shown in figure 1 shown. Depend on figure 1 It can be seen that the polyethylene film has a certain absorption of visible light and ultraviolet light, and at the same time, there is a gradually increasing absorption peak at 250nm-190nm, and an inflection point appears at 220.02nm, and the peak gradually tends to the maximum value. The specific peak intensity and peak shape are shown in the spectrum prevail. The UV-visible absorption spectrum of the polyethylene film obtained after zeroing the instrument with a double-layer polyethylene film...

Embodiment 2 and 3

[0055] Carry out embodiment 1 by the same procedure as described, but in embodiment 2,3, detect respectively the suet and tallow of mentioning.

Embodiment 4

[0057] (1) Put the double-layer food preservation film into the optical path of the UV-visible spectrometer to adjust to zero.

[0058] The ultraviolet-visible spectrum of double-layer food plastic wrap is as follows image 3 shown. Depend on image 3 It can be seen that the preservative film is basically transparent to visible light and ultraviolet rays, and its absorption is smaller than that of polyethylene film, but there is a gradually increasing absorption peak at 250nm-190nm, and an inflection point appears at 215.52nm, and the peak gradually tends to the maximum value. The peak intensity and peak shape are subject to the spectrum. The UV-visible absorption spectra scanned after zeroing the instrument with double-layer plastic wrap and double-layer polyethylene film are as follows: Figure 4 As shown, it can be seen from the ultraviolet-visible spectrum that the baseline is stable after zeroing the instrument with double-layer plastic wrap, but there is a peak that g...

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Abstract

The invention relates to a qualitative detection method with an ultraviolet-visible absorption spectrum of animal oil. The method is characterized in that a polyethylene film is used as a carrier of an animal oil sample for detection, in an ultraviolet-visible spectrum chart of the polyethylene film obtained after zero setting of a double-layer polyethylene film instrument, no absorption peak exists in the range of 190nm to 900nm or the polyethylene film only has the absorbance from 190nm to 217nm in the range of 190nm to 900nm, and the absorbance value is less than or equal to 0.1. The intensity of the absorption peak of the UV-Vis spectrum chart obtained by the detection method is better than that of a food fresh-keeping film, the number of peaks is more than that of an existing solvent-sample quartz cuvette detection method, and the peaks are finer. Meanwhile, the ultra-micro (one in million of scale non-inductive quantity) oil sample, which is remained on the polyethylene film, can be used for obtaining a detection spectrum chart. By the method, the ultraviolet-visible spectrum chart of animal oil can be rapidly, sensitively and accurately measured in a low-cost manner, and the cross infection of samples can be avoided.

Description

technical field [0001] The invention belongs to the field of food inspection and detection, and in particular relates to a qualitative detection method of ultraviolet-visible absorption spectrum of animal oil. Background technique [0002] Animal oil is fat extracted from the meat or tissue of animals. The detection method using ultraviolet-visible absorption spectrum qualitative analysis is to dissolve the oil sample in the solvent of the soluble oil sample, and then scan within the detection wavelength range of the ultraviolet-visible spectrophotometer, that is, 190-900nm, to obtain its ultraviolet-visible spectrophotometer. Visible spectrum, qualitative analysis is carried out according to the peak position, peak shape and peak intensity of the ultraviolet-visible spectrum. [0003] When scanning the UV-Vis spectrum, using different solvents for the same substance will result in different peak positions, peak shapes and peak intensities, that is, the λ of the absorption ...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 姜波胡文忠齐小辉刘长建高明波金黎明
Owner DALIAN NATIONALITIES UNIVERSITY
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