High-density culture technology for oocystis borgei and collection method for oocystis borgei cells

A technology for high-density culture of Poji oocysts, applied in the direction of unicellular algae, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of expensive centrifugal equipment, high operating costs, breaking algal cells, etc., Achieve the effect of not easy to be scratched, not easy to be polluted, and low cost

Active Publication Date: 2014-05-28
江苏津沂菊源生物健康产业研究院有限公司
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Problems solved by technology

[0007] Regarding the method of collecting Oocystella boggie cells, the most commonly used method in the past was centrifuge centrifugation, no pollution, more thorough centrifugation, but the disadvantages are too obvious: centrifugation equipment is expensive, even a simple large centrifuge needs 400,000-50 10,000 yuan, not

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  • High-density culture technology for oocystis borgei and collection method for oocystis borgei cells

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Embodiment Construction

[0018] Culture medium preparation method

[0019] 1) 920 plant growth stimulant is a white crystalline powder, which is difficult to dissolve in water. It can be fully dissolved in alcohol first (each gram is dissolved in 50 ml of alcohol or 60% white wine for more than 1 hour), and then diluted with water according to the required concentration. ;

[0020] 2) In the process of preparing the culture medium, the water needs to be continuously stirred. After adding one kind of nutrient salt to the sterilized seawater, keep stirring, and then add the next one every 3 minutes, and the various nutrient salts are added according to the order provided by the formula. to avoid chemical reactions;

[0021] 3) Various nutrient salts, especially the f / 2 vitamin solution, need to be made into mother liquor in advance, and stored in a 4°C refrigerator for later use;

[0022] 4) Preparation method of sea mud leachate: take the upper soft mud with less sandy quality and more organic matter...

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Abstract

The invention discloses a high-density culture technology for oocystis borgei and a collection method for oocystis borgei cells, and relates to the field of cultivation of marine microalgae, and the traditional culture medium for oocystis borgei is slow in growth rate and low in output. According to the invention, NaNO3 and urea are used as nitrogen sources, proper amount of NaHCO3 is added and 920 plant growth substance, human urine, sea mud extract liquid, cow dung and sheep manure lixivium and soil extract are supplemented, and the nutrition is rather balanced and comprehensive; a domestic used alkaline mineral water bucket culture method replaces the traditional open cement pool culture method, the operation is simple, the possibility of pollution is low, control is easy, and the method is safe and reliable, production is available at all seasons, the cost is low, the growth is quick, the method is particularly suitable for the plant expanding phase, the yield is improved by 180%; the yellow mud method for the collection of oocystis borgei replaces the original centrifugation method, the cell recovery rate is high, the method is simple and feasible, costs little, causes no damage to cells, is suitable for large-scale production and high in economic benefit, the equipment is simple and materials are easily obtained.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a method for high-density cultivation of oocysts boggy and a method for collecting algae cells. Background technique [0002] Oocystis borgei is isolated from the high-level pond of prawns. It is used as a microalgae for microalgae ecological regulation, improvement of the breeding environment, and prevention of prawn diseases. It belongs to the Chlorophyta, Chlorophyceae, Chlorophyceae Chlorococcales, Oocystaceae Oocystaceae, Oocystis Oocystis. Live in groups consisting of 2, 4, 8 cells, the group is oval; there is a layer of obvious gelatin coating outside the group, the cells in the group are ellipse or slightly oval, with wide round ends; the pigment body is sheet-like. The cells are 10-19 μm long and 9-13 μm wide; generally produce 2, 4, 8 or 16 spore-like spores, which are spore-like. [0003] The high level pond is a high-density intensive culture...

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner 江苏津沂菊源生物健康产业研究院有限公司
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