Method for preparing silicosis cell model

Abstract

The invention belongs to the field of medical science, and provides a method which is used for preparing a silicosis cell model from a molecular level, is simple, and can accurately represent pulmonary fibrosis in vivo. The method comprises the specific steps: adopting silicious dust to stimulate alveolar macrophages cultured to a stationary period, after 24 h, collecting a supernatant, filtering, and removing bacteria; and adding the silicious dust and the alveolar macrophage supernatant into lung adenocarcinoma cells A549 cultured to a stationary period, jointly incubating lung epithelial cells with the two substances, and completing establishment of the silicosis cell model.

Description

technical field [0001] The invention relates to a method for preparing a silicosis cell model, which belongs to the field of medicine. technical background [0002] Silicosis is a chronic irreversible disease mainly caused by interstitial pulmonary fibrosis caused by long-term inhalation of silica dust. . Silicosis is the most serious and most frequently occurring occupational disease in China, and its pathogenesis is not yet fully understood. Therefore, an in vitro cell model is prepared to further explore the pathogenesis of silicosis from the cellular and molecular levels, especially the study of cytokines and signal transduction network systems, and its treatment breakthrough is crucial. [0003] At present, in the field of silicosis cell models, there is no accurate and uniform preparation method. In the process of silicosis fibrosis, lung epithelial cells are the main target cells. Researchers usually directly stimulate lung epithelial cells with silica dust, so th...

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Problems solved by technology

[0003] Currently, in the field of silicosis cell models, there is no accurate and uniform preparation method. In the process of silicosis fibrosis, lung epithelial cells are the main target cells. Researchers usually use silicon Dust directly stimulates the lung epithelial cells and damages the epithelial cells. The extracellular matrix and grass-roots adhesive proteins such as type IV collagen and fibronectin (FN) immediately repair and cover the damaged wounds, changing the phenotype of the epithelial cells. Expressing the characteristics of interstitial cells and promoting the formation of fibrous tissue is the main method to simulate the pathological process of pulmonary fibrosis based on pulmonary epithelial cells. However, after the body inhales silica dust, it can also indirectly stimulate alveolar macrophages to release A large number of inflammatory cytokines and fibrotic factors promote alveolar inflammation and gradually transform into pulmonary interstitial fibrosis

The above methods are simple to operate, but there is a certain distance from the pathogenesis of pulmonary fibrosis in vivo. The in vitro cell model of silicosis prepared by a single cell cannot accurately and effectively reflect a series of reactions after silica dust enters the body, thus affecting Subsequent drug intervention experiments, poor accuracy