Automatic device for sample treatment and particle analysis, and method for sample treatment and particle analysis

An automated device, a technology for sample processing, used in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc.

Inactive Publication Date: 2014-05-28
上海纳衍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to overcome the problem of labor consumption and experimental repeatability of sample processing in flow analysis, and the problem of requiring additional labor or equipment to detect the absolute concentration (number / liter) of the target object to be measured. The invention proposes an automatic device and method for sample processing and microparticle analysis, which can automatically and accurately control the processes of labeling reaction and hemolysis reaction, and parameters such as reaction time and reaction dosage can be adjusted by the operator according to needs, and Able to perform absolute counting analysis of the target object to be measured

Method used

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  • Automatic device for sample treatment and particle analysis, and method for sample treatment and particle analysis
  • Automatic device for sample treatment and particle analysis, and method for sample treatment and particle analysis
  • Automatic device for sample treatment and particle analysis, and method for sample treatment and particle analysis

Examples

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Effect test

example 1

[0069] Ask for the proportion and concentration of lymphocytes in the whole blood sample, that is, lymphocytes need to be classified and absolutely counted (number / liter), but no labeling reaction is required.

[0070] Scheme 1: Use a commercial reagent for hemolysis and cell classification reaction, the sample volume involved in the reaction is 50uL, the reagent volume is 1mL, and the reaction time is 10 minutes.

[0071] The experimental operator sets the amount of samples and reagents involved in the reaction and the reaction time on the input device for adjusting the reaction parameters, and the reaction control device accurately controls the whole process of the sample hemolysis and classification reaction according to the parameter settings. The sample suction needle device drives the sample suction needle to the sample injection position, and accurately draws the quantitative sample to be tested; then moves and adds the hemolysis and cell classification reaction pool to ...

example 2

[0075] Find the concentration (number / liter) of the Helper subtype of T lymphocytes in a large number of human whole blood samples, that is, it is necessary to label the whole blood sample with CD3 and CD4 monoclonal antibodies to obtain the percentage of the Helper subtype in the lymphocytes Ratio; and need to obtain the concentration of lymphocytes.

[0076] Protocol: Use a commercial antibody set A (CD3 and CD4 monoclonal antibodies are co-packaged together) for cell labeling reaction. The amount of whole blood sample involved in the reaction is 100uL, the amount of antibody set is 20uL, and the reaction time is 10 minutes.

[0077] A commercial reagent was used for hemolysis and cell classification reaction, the amount of labeled sample involved in the reaction was 50uL, the amount of reagent was 1mL, and the reaction time was 10 minutes.

[0078] A large amount of antibody markers can be pre-stored in the container in the marker storage pool for multiple absorptions, and ...

example 3

[0081] Find the concentration (number / liter) of the Suppressor subclass of T lymphocytes in a small batch of human whole blood samples, that is, you need to label the whole blood sample with CD3 and CD8 monoclonal antibodies to find the percentage of the Suppressor subclass of lymphocytes Ratio; and need to obtain the concentration of lymphocytes.

[0082] Protocol: Use a commercial antibody set B (CD3 and CD8 monoclonal antibodies are packaged separately) for cell labeling reaction. The amount of whole blood sample participating in the reaction is 100uL, the dosage of CD3 monoclonal antibody is 15uL, and the dosage of CD8 monoclonal antibody is 15uL. The reaction time for 15 minutes.

[0083] Self-prepared reagents were used for hemolysis and cell classification reactions. The amount of labeled samples involved in the reaction was 30 uL, the amount of reagents was 1.5 mL, and the reaction time was 7 seconds.

[0084] The marker used for the marking reaction is only stored in...

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Abstract

The invention discloses an automatic device for sample treatment and particle analysis, and a method for sample treatment and particle analysis. A sample pretreatment system in the device comprises a sample pretreatment device, an input device for reaction parameter adjustments, a precise quantitative pipetting and adding device, and a reaction control device; a flow cytometry analysis system in the device can acquire the absolute concentration of a certain kind of targets to be tested. The invention further discloses various work modes in which the device is used for the analysis of the targets to be tested. Through the device and the method disclosed by the invention, the processes of marking reactions, hemolytic reactions and the like can be automatically and precisely controlled, and the parameters such as reaction time and reaction dosage can be adjusted by an operator as needed, so that the experiment consistency and repeatability can be ensured; an absolute concentration analysis of the targets to be tested can be carried out without extra labor or equipment.

Description

1. Technical field: [0001] The present invention relates to devices and methods for sample processing and particle analysis. In particular, the present invention relates to an automatic device and method for sample processing and particle analysis using flow cytometry technology. 2. Background technology: [0002] Flow cytometry technology (Flow Cytometry) applies the principle of hydrodynamic focusing, so that the particles to be analyzed are arranged in a line and flow through the focused light detection area one by one. The light scattered by the particles and the excited fluorescence are captured by multiple Detector detection, after signal processing and sampling, data processing is carried out to realize the classification and counting of target objects. [0003] Flow cytometry analysis technology is widely used in the analysis of various particles. Here, microparticles refer to various cells of humans or other animals and plants and various artificial microspheres, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00C12M1/36C12Q3/00C12Q1/68C12Q1/02
Inventor 陶靖
Owner 上海纳衍生物科技有限公司
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