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A kind of breeding method of fine white tea tree mushroom

A technology of white tea tree mushroom and tea tree mushroom, applied in botany equipment and methods, plant genetic improvement, horticulture, etc., can solve the problems of breeding of white tea tree mushrooms that have not been seen, and achieve the effect of trait improvement

Inactive Publication Date: 2015-10-14
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

The color of the fruiting body of Agrocybe argali has also appeared new traits, and the color has changed from brown to white. At present, the genetics of the white variant of the fruiting body of Agrocybe chinensis is not yet clear, and there is no breeding of white varieties of Agrocybe chinensis.

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  • A kind of breeding method of fine white tea tree mushroom

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Embodiment 1

[0020] Embodiment 1: a kind of breeding method of white tea tree mushroom, comprises the following steps:

[0021] 1. Parent selection

[0022] Agronomic traits analysis of tea tree mushroom and application of EST-SSR marker [D]. Nanchang University, 2012. ).

[0023] 2. Preparation and regeneration of white tea tree mushroom protoplasts

[0024] After the white tea tree mushroom was activated for the second generation on the PDA solid medium, it was transferred to the PDA liquid medium and cultured statically at 25°C for 5 days. When the hyphae grew to about 5mm visible to the naked eye around the inoculation block, pick the mycelium mass into a 1.5mL sterilized centrifuge tube, add 0.6mol / L mannitol 0.5mL, and centrifuge at 4°C, 5000rpm for 5min. Remove the supernatant, add 0.5 mL of 2% lysozyme, and incubate in a constant temperature water bath at 28°C for 4 hours, shaking once every half hour. After 4 hours of enzymolysis, the enzymolysis solution was filtered thro...

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Abstract

The invention belongs to the field of agrocybe cylindracea, and particularly relates to a breeding method for excellent white agrocybe cylindracea. The method comprises the steps that hybridization is carried out on the white agrocybe cylindracea and the agrocybe cylindracea of a pale taupe strain, secondary hybridization is carried out on filial generations and the white agrocybe cylindracea again, and hybrids of the white agrocybe cylindracea can be obtained. The obtained hybrids of the white agrocybe cylindracea carry genes of the agrocybe cylindracea of the pale taupe strain, the excellent character of the agrocybe cylindracea of the pale taupe strain can be inherited to the white agrocybe cylindracea, and therefore the character of the white agrocybe cylindracea is improved.

Description

technical field [0001] The invention belongs to the field of tea tree mushroom, and more specifically relates to a breeding method of fine white tea tree mushroom. Background technique [0002] tea tree mushroom ( Agrocybe cylindracea ) belongs to Basidiomycotina, Phylomycetes, Agaricaceae, Amanitaceae, and it is a kind of woody edible fungus. It grows in temperate to subtropical regions and has high nutritional value. It has a delicious taste, a fat cover and a crisp handle, a delicious fragrance, a unique flavor, and rich nutrition. It is loved by people. It is also a traditional folk medicinal fungus. Antidiarrheal, heat-clearing and calming liver effects, enjoy the title of "Chinese mushroom". [0003] Tea Tree Mushroom was originally a wild fungus. It is mainly distributed in Camellia oleifera forests in mountainous areas of Jiangxi, Fujian, Zhejiang, Guizhou, Yunnan, Taiwan and other provinces in China. The fruiting body is dark reddish brown or light brown at the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04A01H1/02A01H1/04A01H15/00
Inventor 刘新锐谢宝贵王圣铕
Owner FUJIAN AGRI & FORESTRY UNIV
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