An Ultra-High Resolution Nonlinear Optical Microscopy System

Abstract

The invention discloses an ultrahigh-resolution non-linear optical microscopy system, and relates to the field of laser detection. According to the system, a non-linear optical technique and a stimulated emission depletion microtechnique are combined, a beam of short pulse infrared laser is used as a multi-photon stimulated light source, another beam of super-short laser pulse of a wide tuning range, which is generated from a synchronous short pulse laser pumping frequency doubling pumping light parameter oscillator, is expanded to be 200ps in pulse width by virtue of a single-mode optical fiber; as a stimulated emission depletion light source, the stimulated light is used for stimulating fluorescent molecules; the fluorescence at the periphery of a stimulated light spot focus is quenched by virtue of the stimulated emission depletion light, and then the non-linear optical microscopy system can break through the light wave diffraction limit. According to the system, in the non-linear optics, infrared laser with long wavelength is used as the multi-photon stimulated light source with high penetration depth because of small scattering influence, and ultrahigh resolution tomography imaging of a living organism tissue is facilitated.

Description

technical field [0001] The invention relates to the field of laser detection, in particular to an ultra-high resolution nonlinear optical microscope system. Background technique [0002] The spatial resolution of far-field optical microscopy has been limited by the diffraction limit. Due to its wave characteristics, light waves will be diffracted, so the beam cannot be infinitely focused. 200nm is the theoretical resolution limit of the far-field optical microscope. With the rapid development of life sciences, the research has penetrated into the level of single cell, sub-cell and single molecule, and the spatial resolution of optical microscope has become the most critical core issue. [0003] Although Near-field Scanning Optical Microscopy (Near-field Scanning Optical Microscopy, NSOM) breaks through the diffraction limit, is no longer limited by light waves, and the resolution can reach the nanometer level, but it is only suitable for two-dimensional high-resolution meas...

AI Technical Summary

Problems solved by technology

One of the bottlenecks encountered in the development of ultra-high resolution optical microscopy is that it is difficult to image living tissues

The essence is that these microscopic methods are based on single-photon excitation fluorescence technology, which has poor optical penetration and cannot meet various needs in practical applications.

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