Method for identifying male and female plants of populus tomentosa seedlings

A technology of Populus tomentosa and male and female plants, which is applied in the field of identification of male and female plants, can solve the problems of complicated operation procedures, expensive medicines, and unsuitable promotion, and achieve the effects of obvious color changes, simple and easy method, and shortened identification time

Inactive Publication Date: 2014-07-16
LIAONING UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The accuracy of isozyme spectrum and chromosome karyotype identification is high, but the difference in enzyme bands is not necessarily caused by male and female sex, it may be related to the variation of other traits, such as leaf traits, etc. These variations are common in male and female Populus tomentosa Exist, and because in the early stage of identification, there are fewer enzymes to choose from, and there are not many colored enzyme bands, sometimes it is impossible to distinguish between male and female plants
Moreover, the operation process is complicated, requiring CTAB, PVP, Tris-HCl, phenol, chloroform, TE, ethidium bromide and other drugs. The drugs are expensive and highly toxic, and primers and PCR instruments are required. It is difficult for ordinary laboratories to operate with knowledgeable people
Therefore, the above methods are not suitable for promotion in urban landscaping projects.

Method used

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  • Method for identifying male and female plants of populus tomentosa seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Effects of temperature and pH value on the identification method of male and female Populus tomentosa

[0025] (1) Materials and methods

[0026] Take 10 20-year-old mature Populus tomentosa leaves with known male and female plants and 10 3-year-old Populus tomentosa seedlings with known male and female leaves, wash them, dry them, and set them aside.

[0027] Weigh 0.5-1.0g Populus tomentosa leaves, cut them into pieces, add 4-5ml phosphate buffer solution, grind in an ice bath to form a homogenate, extract at room temperature for 1-1.5h; centrifuge the extract at 5000rpm for 5min. Take 2ml of the supernatant, add 4-5ml of 0.1% litmus test solution, mix well, and observe the influence of the ambient temperature and the pH value of the phosphate buffer on the color change of the extract.

[0028] (2) Effect of temperature on color change

[0029] The pH of the phosphate buffer was fixed at 6.8, and the color change of the extract was observed at 20°C, 25°C, ...

Embodiment 2

[0037] Embodiment 2 A kind of identification method of Populus tomentosa seedling male and female plants

[0038] 1. The steps are as follows:

[0039] (1) Preparation of materials:

[0040] 1) Select the robust leaves of Populus tomentosa seedlings;

[0041] 2) The leaves are washed with running water for 4-5 minutes;

[0042] 3) absorb moisture;

[0043] 4) Seal it with a fresh-keeping bag;

[0044] 5) Store in refrigerator at 4°C for later use;

[0045] (2) Detection method:

[0046] 1) prepare phosphate buffer, the method is as follows: NaH 2 PO 4 ·H 2 Add water to O27.8g to make 1000ml solution, Na 2 HPO 4 ·7H 2 Add water to O53.65g to make 1000ml solution; take 62.5ml NaH 2 PO 4 ·H 2 O solution and 37.5ml Na 2 HPO 4 ·7H 2 O solution was mixed and diluted with water to 200ml to obtain a phosphate buffer solution with a concentration of 0.2mol / L and a pH of 6.8;

[0047] 2) Prepare 0.1% litmus test solution: dissolve 0.1g of litmus in 50ml of water, let i...

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Abstract

The invention provides a method for identifying male and female plants of populus tomentosa seedlings. Identification is carried out mainly aiming at the seedling stage of male and female plants of populus tomentosa seedlings in urban garden greening. Various conditions required for preliminary identification of the male and female plants of the populus tomentosa seedlings are ensured by preparing a phosphate buffer solution, utilizing chromogenic reaction of a litmus test solution, and proper treatment at the seedling stage. An effective method, which can be applied in large area in production practice of urban garden greening, can solve the problem for identifying the male and female plants of the populus tomentosa seedlings, is simple and convenient to operate, and high in accuracy rate, shortens the identification time and saves the cost is provided by the steps of grinding, leaching and centrifuging the male and female plants of the populus tomentosa seedlings.

Description

technical field [0001] The invention relates to a method for identifying male and female plants of plants, in particular to an early identification method for male and female poplar plants. Background technique [0002] Most plants in nature are monoecious, but a few tree species such as poplar tomentosa are dioecious; male poplar tomentosa only blooms but does not bear fruit, and female poplar tomentosa will bear fruit; even if the branches of female poplar tomentosa are grafted to male poplar tomentosa On the ground, only this branch bears fruit. Generally, it is difficult to distinguish male and female poplar trees from the appearance. There is only a slight difference in tree shape between the two. The crown of the female poplar expands to both sides, while the crown of the male poplar grows desperately upwards. Only professionals in the industry can distinguish between male and female plants. Due to the lack of fruit, many male poplars are cut down. Therefore, in natu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 李悦陈忠林张利红侯伟徐苏男张丽梅张雨
Owner LIAONING UNIVERSITY
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