Culture method for increasing yield of capsule of avian pasteurella multocida

A poultry-derived multi-kill, Pasteurella technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve the problems of cumbersome solid culture method, unsuitable for large-scale production, etc., and achieve high yield. Effect

Active Publication Date: 2014-08-06
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid culture method harvests more capsules and lower cost, but the sol

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture method for increasing yield of capsule of avian pasteurella multocida

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A culture method for improving the capsule yield of Pasteurella multocida of avian origin, comprising the following steps:

[0028] (1) Inoculation:

[0029] Pasteurella multocida of avian origin is inoculated in the 500mL Erlenmeyer flask that culture medium is housed, and the inoculum amount of the Pasteurella multocida seed liquid of said avian origin is 1% (4mL) of the total volume of the culture medium, and the The filling capacity of the medium is 4 / 5 (400mL) of the volume of the Erlenmeyer flask;

[0030] (2) shaking culture:

[0031] Place the inoculated Erlenmeyer flask in step (1) in an incubator for shaking culture, the culture temperature is 37°C, the shaker speed is 200rpm, and the shaking culture time is 6h;

[0032] (3) static culture

[0033] The Erlenmeyer flask cultured by shaking in step (2) was placed in an incubator to stand still, the culture temperature was 37° C., and the culture time was 8 hours.

[0034] The above-mentioned medium preparati...

Embodiment 2

[0037] A culture method for improving the capsule yield of Pasteurella multocida of avian origin, comprising the following steps:

[0038] (1) Inoculation:

[0039] Pasteurella multocida of avian origin is inoculated in the 500mL Erlenmeyer flask that culture medium is housed, and the inoculum amount of the Pasteurella multocida seed liquid of said avian origin is 1% (4mL) of the total volume of the culture medium, and the The filling capacity of the medium is 4 / 5 (400mL) of the volume of the Erlenmeyer flask;

[0040] (2) shaking culture:

[0041] Place the Erlenmeyer flask inoculated in step (1) in an incubator for shaking culture, the culture temperature is 37°C, the shaker speed is 200rpm, and the shaking culture time is 5.5h;

[0042] (3) static culture

[0043] The Erlenmeyer flask cultured by shaking in step (2) was placed in an incubator to stand still, the culture temperature was 37° C., and the culture time was 8.5 hours.

[0044] The above-mentioned medium prepa...

Embodiment 3

[0047] A culture method for improving the capsule yield of Pasteurella multocida of avian origin, comprising the following steps:

[0048] (1) Vaccination:

[0049] Pasteurella multocida of avian origin is inoculated in the 500mL Erlenmeyer flask that culture medium is housed, and the inoculum amount of the Pasteurella multocida seed liquid of said avian origin is 1% (4mL) of the total volume of the culture medium, and the The filling capacity of the medium is 4 / 5 (400mL) of the volume of the Erlenmeyer flask;

[0050] (2) shaking culture:

[0051] The Erlenmeyer flask inoculated in step (1) was placed in an incubator for shaking culture, the culture temperature was 37° C., the shaker speed was 200 rpm, and the shaking culture time was 6.5 h;

[0052] (3) static culture

[0053] The Erlenmeyer flask after shaking culture in step (2) was placed in an incubator to stand still, the culture temperature was 37° C., and the culture time was 7.5 hours.

[0054]The above-mentioned...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a culture method for increasing the yield of the capsule of avian pasteurella multocida. The culture method comprises the following steps: (1) inoculation: inoculating avian pasteurella multocida into a triangular flask with a culture medium; (2) shake culture: putting the triangular flask after inoculation in the step (1) into an incubator to undergo shake culture at a temperature of 36-38 DEG C for 5.5-6.5 hours, wherein the shaker speed is 180-220rpm; (3) stationary culture: putting the triangular flask after shake culture in the step (2) into the incubator to stand, wherein the culture temperature is 36-38 DEG C and the stationary culture time is 7.5-8.5 hours. The yield of the capsule of avian pasteurella multocida can be obviously increased by adopting the culture method provided by the invention.

Description

technical field [0001] The invention relates to a cultivation method for increasing the capsule yield of Pasteurella multocida from avian origin. Background technique [0002] Fowl cholera, also known as avian hemorrhagic septicemia, avian pasteurellosis or poultry shaking head plague, is a contagious disease that infects various poultry caused by Pasteurella multocida. The disease is often manifested as a septic type, with high morbidity and mortality, and also a chronic type, which causes significant economic losses to the poultry industry worldwide. Pasteurella multocida is a Gram-negative, non-flagellate, non-spore-forming small bacillus. The newly isolated strain has a capsule, which can enhance the invasiveness and reproductive ability of the strain. [0003] Cultivate Pasteurella multocida from avian origin with Martin broth or agar containing 3%-5% calf serum and 0.25% glucose, extract its capsule to make fowl cholera capsule subunit vaccine, and the recent immune p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12R1/01
Inventor 程龙飞林建生黄瑜傅光华施少华陈红梅万春和傅秋玲
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap