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Pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method

A technology of green spheres, ferns, used in the direction of botany equipment and methods, plant regeneration, gardening methods

Active Publication Date: 2014-08-13
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The green spheroids of ferns have not been reported for radiation mutagenesis

Method used

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  • Pteridophyte green spheroid &lt;60&gt;Cor ray irradiation mutagenesis method
  • Pteridophyte green spheroid &lt;60&gt;Cor ray irradiation mutagenesis method
  • Pteridophyte green spheroid &lt;60&gt;Cor ray irradiation mutagenesis method

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Experimental program
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Effect test

Embodiment Construction

[0028] 1. Materials and Methods

[0029] 1.1 Test material

[0030] Aseptic seedlings of the ferns Pteridophyllum and Pteridophyllum were purchased from the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. The green spheroids (greenglobularbodies, referred to as GGB) induced by sterile vaccines were used as materials, 60 Cor rays are radiation sources.

[0031] 1.2 Medium

[0032] The basal medium consists of MS macroelements, MS trace elements (MurashigeT, SkoogF.Arevised medium from rapid growth and bioassays with to baccotissue cultures[J].Physiol plant,1962,15:473~497) and B 5 Vitamin (GamborgOL, MillerRA, OjimaK.Nutrientrequirementsofsuspensionculturesofsoybeanrootcells[J].Exp.CellRes., 1968.50:151~158) composition, is a kind of medium commonly used in plant tissue culture, sucrose 30g / L, agar bar (Chengfeng brand, Fujian Quanzhou Quangang Chemical Factory) 8g / L;

[0033] Green spheroid induction and proliferation medium additi...

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Abstract

The invention relates to a pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method, and belongs to the technical field of a plant cultivation method. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method is characterized in that a green spheroid generated by inducing a pteridophyte aseptic seedling under the condition of isolated culture is used as a material, and <60>Cor rays are used as radiation sources; the green spheroid subjected to irradiation treatment is transferred to a grow-up seedling culture medium containing 0.2mg / L of additional indolebutyric acid (IBA) and germinates, thus a complete plantlet with roots, stems and leaves is formed; the dosage rate of the<60>Cor rays is 1Gy / min, the irradiation dose is 80Gy, and the seedling rate of the green spheroid of neocheiropteris palmatopedata is 38%; the seedling rate of the green spheroid of pteris multifida poir is 26%. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method provided by the invention provides a new technology for development of irradiation mutagenesis breeding of pteridophyte on the organ level and is suitable for breeding of pteridophyte new varieties.

Description

technical field [0001] Fern green spherical body of the present invention 60 Cor ray radiation mutagenesis method relates to a method suitable for plant tissue culture and 60 The radiation mutagenesis technology of Cor ray belongs to the technical field of plant cultivation methods. Background technique [0002] Ferns, also known as ferns, originated from the bare ferns of the Silurian and Devonian periods of the Paleozoic Era. The taxonomic status of ferns is between bryophytes and seed plants. At present, there are more than 12,000 species in the world, mainly distributed in tropical and subtropical regions, and there are more than 2,600 species in China (Qin Renchang, 1959, Flora of China (Volume II) ).Beijing: Science Press). The reproduction methods of ferns are divided into sexual reproduction (spore reproduction), asexual reproduction (vegetative reproduction) and tissue culture, and the breeding method is introduction and domestication (Han Jing, Zhao Li. Research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 叶晓青佘建明邓衍明贾新平梁丽建孙晓波
Owner JIANGSU ACAD OF AGRI SCI
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