A retrograde microbial fuel cell device using ultrasonic pretreatment of electrogenic microorganisms
A technology of electricity-producing microorganisms and fuel cells, which is applied to biochemical fuel cells, fuel cells, fuel cell components, etc., can solve problems such as low electricity generation efficiency and slow oxidation rate, and achieve improved electricity generation performance and mass transfer performance, and the effect of improving the efficiency of electricity production and wastewater treatment
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Embodiment 1
[0044] Embodiment 1: A kind of back-type microbial fuel cell device using ultrasonic pretreatment of electricity-producing microorganisms of embodiment 1 comprises the following steps:
[0045] (1) Preparation of electrodes
[0046] a. Anode preparation
[0047] Take two pieces of carbon felt with a specification length×width of 4.0×4.0cm and a thickness of 1.0cm, and a copper sheet with a specification length×width of 4.0×4.0cm is sandwiched between them, and the copper sheet is fixed on the carbon felt with carbon fiber wire as an anode ;
[0048] b. Cathode preparation
[0049] Use a copper sheet with a size of 4.0×4.0cm in length×width as the cathode;
[0050] (2) Cultivation of anode activated sludge and cathode Pseudomonas culture solution
[0051] a. Anode activated sludge cultivation
[0052] Add 200 g of granular activated sludge purchased from Shanghai Daocheng Environmental Engineering Co., Ltd. into 800 mL of culture medium. The dosage of each component of the...
Embodiment 2
[0072]Embodiment 2: (1), (2) (5) and (6) steps are identical with embodiment 1 in the step of a kind of back-type microbial fuel cell device that uses ultrasonic wave to pretreat electricity-producing microorganisms of embodiment 2, its ( 3) and (4) steps are different, the specific steps are:
[0073] (3) Ultrasonic pretreatment
[0074] 20mL of the anode activated sludge obtained through step 2 (a) cultivation and 20mL of the Pseudomonas schizophrenia culture liquid obtained through step 2 (b) cultivation are carried out ultrasonic treatment with an ultrasonic cell pulverizer respectively, and the ultrasonic power is set as 400W, ultrasonic time is 15min;
[0075] (4) Immobilization of anode activated sludge and cathode Pseudomonas culture solution
[0076] a. Immobilization of anode activated sludge
[0077] Take 13g of polyethylene glycol and dissolve it in a solution containing 0.7% N, N, N , , N , - In 150mL of demineralized water of tetramethylene diamine and 0.8g ...
Embodiment 3
[0080] Embodiment 3: (1), (2) (5) and (6) steps are identical with embodiment 1 in the step of a kind of back-type microbial fuel cell device that uses ultrasonic wave to pretreat electricity-producing microorganisms of embodiment 3, its ( 3) and (4) steps are different, the specific steps are:
[0081] (3) Ultrasonic pretreatment
[0082] 20mL of the anode activated sludge obtained through step 2 (a) cultivation and 20mL of the Pseudomonas schizophrenia culture liquid obtained through step 2 (b) cultivation are carried out ultrasonic treatment with an ultrasonic cell pulverizer respectively, and the ultrasonic power is set as 600W, ultrasonic time is 20min;
[0083] (4) Immobilization of anode activated sludge and cathode Pseudomonas culture solution
[0084] a. Immobilization of anode activated sludge
[0085] Take 14g of polyethylene glycol and dissolve it in a solution containing 0.8% N, N, N , , N , - In 150mL of demineralized water of tetramethylene diamine and 0.8g...
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