Tissue culture and propagation method for Spiranthes sinensis Ames

A technology of tissue culture and Panlong ginseng, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of small natural population of Panlong ginseng, hinder the development of medicinal value, and difficult to enrich active ingredients, etc., to achieve Good sterilization effect, high value-added multiple, and simple method

Inactive Publication Date: 2014-08-27
大兴安岭林业集团公司农业林业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The natural population of Panlong ginseng is very small, and no large-scale Panlong ginseng community has been found at present. The germination rate of its seeds is low under natural conditions, which brings difficulties to artificial cultivation. Enrichment, hindering the development of medicinal value
There are few reports about the tissue culture of Panlong ginseng

Method used

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  • Tissue culture and propagation method for Spiranthes sinensis Ames
  • Tissue culture and propagation method for Spiranthes sinensis Ames
  • Tissue culture and propagation method for Spiranthes sinensis Ames

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specific Embodiment approach 1

[0023] Specific embodiment one: the method for Panlong ginseng tissue culture propagation in the present embodiment is carried out according to the following steps:

[0024] Step 1, take Panlong ginseng as an explant, wash it with tap water for 5 minutes, soak it with 75% (volume) alcohol for 1 minute, then soak it with 0.1% (mass) mercuric chloride for 4 minutes, sterile Rinse with water 3-5 times;

[0025] Step 2: Inoculate the explants in the primary induction medium (see figure 1 ), the first-generation induction medium: improved MS+IAA1.5mg / L+6-BA0.5mg / L+sucrose 30g / L+agar powder 6.8g / L, gave 2000LX light, and the light time was 12 hours / day, and cultivated The temperature is 22-24°C, and the culture is 20-25 days; see the photo of the expansion after 15 days of inoculation. figure 2 , see photos of inoculation 25 days image 3

[0026] Step 3, then inoculate on the secondary proliferation medium, the secondary proliferation medium: MS+BAP2.0mg / L+GA 3 0.5mg / L + sucr...

specific Embodiment approach 2

[0047] Specific embodiment two: the method for Panlong ginseng tissue culture propagation in the present embodiment is carried out according to the following steps:

[0048] Step 1, take Panlong ginseng as an explant, wash it with tap water for 5 minutes, soak it with 75% (volume) alcohol for 1 minute, then soak it with 0.1% (mass) mercuric chloride for 4 minutes, sterile Rinse with water 3 times;

[0049] Step 2, inoculate the explants in the first-generation induction medium, the first-generation induction medium: improved MS+IAA1.5mg / L+6-BA0.5mg / L+sucrose 30g / L+agar powder 6.8g / L, give The light of 2000LX, the light time is 12 hours / day, the culture temperature is 22-24°C, and the culture is 20-25 days.

[0050] Step 3, then inoculate on the secondary proliferation medium, the secondary proliferation medium: MS+BAP2.0mg / L+GA 30.5mg / L + sucrose 30g / L + agar powder 6.8g / L, culture temperature 25°C, give 2500LX light at first, irradiate with red light with light intensity 10...

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Abstract

The invention discloses a tissue culture and propagation method for Spiranthes sinensis Ames, and belongs to the field of artificial cultivation of the Spiranthes sinensis Ames. According to the method, an explant can be well sterilized and is high in survival rate, the multiplication of multiple shoots is high, the rooting rate of tissue culture seedlings is high, and the survival rate of transplanting is high. The method comprises the steps that firstly, an annual underground kryptoblast of the Spiranthes sinensis Ames serves as the explant, and the explant is sterilized and is washed with sterile water 3-5 times; secondly, initial induction culture is carried out; thirdly, successive multiplication is carried out; fourthly, the multiple shoots obtained in the successive multiplication process are cut into single plants, and then rooting culture is carried out; fifthly, the culture is moved under natural illumination in a closed mode to be trained for 5-6 days, and then the seedlings are trained for 1-2 days in an open mode, transplanted into a transplantation medium and managed to grow to grown-up seedlings. Thus, tissue culture and propagation of the Spiranthes sinensis Ames are completed. The method is simple, fast to implement, economical, practical and high in operability, the purpose of rapid propagation is achieved, and the method can be applied to industrialized seedling culture of the orchid Spiranthes sinensis Ames.

Description

technical field [0001] The invention belongs to the field of artificial cultivation of Panlong ginseng, and specifically relates to a method for tissue culture and propagation of Panlong ginseng. Background technique [0002] Spiranthesinensis, Orchidaceae, Spiranthesinensis genus, scientific name Spiranthessinensis. (Pers.) Ames. Orch., also known as Spiranthessinensis. Terrestrial orchid, 15-20 cm high, with erect stems and several thick fleshy roots clustered at the base. Born in wet grassland, hillside forest or ditch edge grass. There are wild distributions in Huzhong, Tahe and Jiagedaqi in the Daxinganling area of ​​Heilongjiang Province. Panlong ginseng root or whole herb is used as medicine, mainly containing coumarin and alkaloids. Sweet, light, flat, nourishing yin and Qi, cooling blood and detoxification, astringent essence, strong. [0003] The natural population of Panlong ginseng is very small, and no large-scale Panlong ginseng community has been found at ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 金凤新刘德文齐会娟湛鑫琳金屿淞吕文博万群芳陈冰
Owner 大兴安岭林业集团公司农业林业科学研究院
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