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Kit for detecting sodium borate in food

The technology of a kit and borax is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., which can solve the problems of high detection sensitivity, cumbersome operation steps and low detection efficiency, etc. To achieve the effect of fast color development, simple reagents and easy operation

Inactive Publication Date: 2014-08-27
南京麦思德餐饮管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The curcumin method has high detection sensitivity and low cost, but the color development must be carried out in an organic solvent during the experiment, and the operation steps are cumbersome, which limits its application to a certain extent. -8 h and to be measured in oxalic acid ethanol solution, the whole experimental process is time-consuming and labor-intensive, and the detection efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take jelly powder purchased in the market, weigh 50g, take the grinding particles in the kit and a disposable grinding rod to grind, add 25ml of reagent 1, soak for 10min, use a disposable syringe filter to filter the crude extract of step (1), and get Serum to be tested. Take 1ml of the supernatant, add 1ml of reagent 2 Alizarin Red S solution, mix well, and let it stand for 1 minute. Take another 1ml of reagent 1, add 1ml of alizarin red S solution, mix well, and use it as a control. The contrast solution and the test solution were compared side by side with the naked eye, and the color of the test solution changed to yellow, indicating that borax was added to the jelly. Further, compare the color of the solution to be tested with the color card side by side with the naked eye to determine the borax content.

Embodiment 2

[0035] Weigh 50g of beef purchased in the market, grind the abrasive particles and disposable grinding rods in the kit, add 25ml of reagent 1, soak for 10min, and filter the crude extract in step (1) with a disposable syringe filter to obtain Serum to be tested. Take 1ml of the supernatant, add 1ml of reagent 2 Alizarin Red S solution, mix well, and let it stand for 1 minute. Take another 1ml of reagent 1, add 1ml of alizarin red S solution, mix well, and use it as a control. Put the control solution and the test solution side by side for visual color comparison, and the color of the test solution changes to yellow, indicating that borax is added to the cold beef. Further, compare the color of the solution to be tested with the color card side by side with the naked eye to determine the borax content.

Embodiment 3

[0037] Buy bean curd sticks from the market, weigh 50g, take the grinding particles in the kit and a disposable grinding rod to grind, add 100ml of reagent 1, soak for 30min, use a disposable syringe filter to filter the crude extract of step (1), and get Serum to be tested. Take 1ml of the supernatant, add 1ml of reagent 2 Alizarin Red S solution, mix well, and let it stand for 1 minute. Take another 1ml of reagent 1, add 1ml of alizarin red S solution, mix well, and use it as a control. The contrast solution and the solution to be tested were compared with the naked eye, and the color of the solution to be tested changed to yellow, indicating that borax was added to the bean curd sticks. Further, compare the color of the solution to be tested with the color card side by side with the naked eye to determine the borax content.

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PUM

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Abstract

The invention provides a kit for detecting sodium borate in food. The kit for detecting the sodium borate in the food comprises a reagent 1, a reagent 2, grinding particles, a disposable grinding rod, a disposable syringe-driven filter and a color chart card, wherein the reagent 1 is a phosphate buffer solution with pH 8.0, and the reagent 2 is an alizarin red S solution. The kit for detecting the sodium borate in the food is simple in reagent, low in cost, easy and convenient to operate, easy to grasp by people and suitable for widely use.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a kit for detecting borax in food. Background technique [0002] Boron is a trace element that widely exists in the biological world, and plant growth, especially grain plant growth, requires boron element. Borax, also known as moonstone sand, yellow moon sand, chemically named sodium tetraborate, forms boric acid under acidic conditions, is a very common industrial raw material, does not belong to the scope of food additives and food additives, so it cannot be used in food and Add such substances. Borax is one of the non-edible substances in the announcement of the National Ministry of Health ((The list of non-edible substances that may be illegally added in food and food additives that are easily abused (the first batch)). Long-term consumption of foods containing borax may cause its Accumulate in organs, leading to chronic poisoning, the main manifestations are anor...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 闻献
Owner 南京麦思德餐饮管理有限公司
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