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Method for measuring methyl anthranilate in spicery

A technology of methyl anthranilate and methyl anthranilate, which is applied in the field of determination of methyl anthranilate in flavors and fragrances, can solve the problems of false positives, accurate qualitative confirmation and quantitative analysis of unfavorable target compounds, and improve the The effect of accuracy, reducing error, and ensuring accuracy

Active Publication Date: 2014-08-27
CHINA TOBACCO FUJIAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For relatively simple matrices, the above method can accurately detect target compounds, but for complex systems such as flavors and fragrances, the above method has serious "false positive" problems, which is not conducive to accurate qualitative confirmation and quantitative analysis of target compounds

Method used

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  • Method for measuring methyl anthranilate in spicery
  • Method for measuring methyl anthranilate in spicery
  • Method for measuring methyl anthranilate in spicery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The detection method of the present embodiment to the content of methyl anthranilate in essence and fragrance is as follows:

[0030] (1) Preparation of internal standard solution

[0031] Weigh 20 mg of internal standard naphthalene, place it in a 500 mL volumetric flask, dissolve and constant volume with dichloromethane, and obtain an internal standard concentration of 0.04 mg / mL. The internal standard solution should be stored in the dark at 4°C, and placed at room temperature when taking it, and can be used after reaching room temperature, and the validity period is 3 months.

[0032] (2) Preparation of standard working solution

[0033] Accurately weigh 54.84mg of methyl anthranilate standard substance, place it in a 500mL brown volumetric flask, dissolve and constant volume with dichloromethane, and obtain methyl anthranilate standard solution (109.86μg / mL). Accurately draw 0.1mL, 0.2mL, 0.5mL, 1mL, 2mL, and 5mL of methyl anthranilate standard solution into a 10...

Embodiment 2

[0053] Detection of precision and spike recovery

[0054] Add 10mg / kg, 20mg / kg, and 50mg / kg standard solutions of three different concentrations to the flavor and fragrance samples in which methyl anthranilate was detected, respectively, to carry out the standard addition recovery test, each sample was measured 6 times, and the chromatographic The conditions of mass spectrometry are the same as in Example 1, and the recovery rate of methyl anthranilate in flavors and fragrances of this method is calculated according to the analysis results, and the results are shown in Table 1.

[0055] Table 1 Recovery and repeatability of methyl anthranilate in flavors and fragrances (n=6)

[0056]

[0057] As can be seen from Table 1, at the three spiked levels, the recovery rate of sesamol in tobacco flavors and fragrances detected by this method is between 90.5% and 103.4%, and the relative standard deviation of the sample test results is less than 5%, indicating that The recovery rat...

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Abstract

The invention discloses a method for measuring methyl anthranilate in spicery. The method comprises the steps of preparation of internal standard solutions, preparation of standard working solutions, preparation of sample solutions, analysis of gas chromatographic mass spectrometry, drawing of a standard curve, and result calculation. The internal standard method is adopted by the method for quantification, operation is easy and accurate, errors caused by sample pretreatment, instrument precision and the like can be effectively reduced, the adopted chromatographic conditions enable chromatographic peaks of an object and an internal standard substance to be well separated, the good linear dependence is obtained, the detection limit is 0.12 mg / kg, the quantitation limit is 0.36 mg / kg, the average relative standard deviation is smaller than five percent, adding standard recovery ranges from 90.5 percent to 103.4 percent, and the method is high in sensitivity, good in repeatability and high in recycling rate.

Description

technical field [0001] The invention belongs to the technical field of detection of flavors and fragrances, and in particular relates to a method for measuring methyl anthranilate in flavors and fragrances. Background technique [0002] Methyl anthranilate, also known as neroli oil, is used to prepare essences such as neroli and honeysuckle, artificial strawberry, and artificial grape. The pure product is a colorless to pale yellow liquid with blue fluorescence and a fruity aroma of orange blossom. Methyl anthranilate is an irritant and may cause respiratory and skin disorders at high levels. [0003] In order to ensure product quality and safety, it is necessary to closely monitor and monitor the quality and safety of flavors and fragrances that may be added. High attention should also be paid to substances that are clearly prohibited in management regulations and even substances that may be harmful to human health. Since methyl anthranilate is a common artificial flavor,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 邓其馨苏明亮刘江生叶仲力方钲中林艳梁晖谢卫
Owner CHINA TOBACCO FUJIAN IND
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