Method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase

A technology of carbonic anhydrase and calcium carbonate scale, applied in chemical instruments and methods, biological water/sewage treatment, complex/solubilizer treatment, etc., can solve problems such as environmental pollution and water eutrophication

Active Publication Date: 2014-10-01
CHONGQING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, on the one hand, phosphate is a nutrient source for microorganisms in the circulating water system, which is conducive to the growth of microorganisms to form soft scale; on the other hand, phosphate is discharged into the water body along with the sewage of the circulating water system, resulting in eutrophication of the water body
At present, nearly 100,000 tons of phosphorus are used for the production of corrosion and scale inhibitors in the country every year. These phosphorus are discharged into the environment almost without any treatment, causing pollution to the environment.

Method used

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  • Method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase
  • Method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase
  • Method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 The method of the present invention removes calcium carbonate scale

[0030] 1) Preparation of bacterial agent: take an appropriate amount of cooling water from the circulating water system, and evenly coat it on the 60g / L calcium carbonate, 1μmol / LZnSO 4 On the beef extract peptone agar plate, place the plate at 34~37°C for 24h, pick out the grown colonies and use the streaking method for purification.

[0031] 2) Expansion culture: Inoculate the microorganism (Bacillus subtilis) purified in step 1) into LB liquid medium to expand the culture to a stable period, the culture temperature is 30±1°C, and the shaker speed is 120r / min.

[0032] 3) Extraction of carbonic anhydrase crude enzyme solution: Centrifuge the bacterial solution cultured in 2) at 4° C. and 5000 r / min for 10 minutes to remove cells. Add appropriate amount (NH 4 ) 2 SO 4 The solution saturation reached 70%, and after salting out at 4℃ for 12h, the protein was obtained by centrifugation at 8000r / min f...

Embodiment 2

[0036] Example 2 The method of the present invention removes calcium carbonate scale

[0037] 1) Preparation of bacterial agent: take an appropriate amount of cooling water from the circulating water system, and evenly coat it on the 60g / L calcium carbonate, 1μmol / LZnSO 4 On the beef extract peptone agar plate, place the plate at 34~37℃ for 24h, pick out the grown colonies and use the streaking method for purification.

[0038] 2) Expansion culture: Inoculate the microorganism (Bacillus subtilis) purified in step 1) into LB liquid medium to expand the culture to a stable period, the culture temperature is 30±1°C, and the shaker speed is 120r / min.

[0039] 3) Extraction of carbonic anhydrase crude enzyme solution: Centrifuge the cultured bacterial solution in 2) at 4° C. and 5000 r / min for 10 minutes to remove cells. Add appropriate amount (NH 4 ) 2 SO 4 The solution saturation reached 70%, and after salting out at 4℃ for 12h, the protein was obtained by centrifugation at 8000r / min fo...

Embodiment 3

[0042] Example 3 Treatment of scale formed in pipes of circulating water system in a chemical plant

[0043] Before treatment, the system fouled seriously, and the massive fouling closely adhered to the inner wall of the system, which caused blockage of the pipeline and reduced heat transfer efficiency. The microorganisms that secrete extracellular carbonic anhydrase were expanded and cultivated to an OD600nm value above 0.8, and the crudely extracted carbonic anhydrase was put into the circulating water system of the factory. After 10 days of normal operation, it was found that the massive scale on the inner wall of the system appeared loose and followed As the scouring of the circulating water falls off into the collecting tank, the pipe blockage has been improved, and the heat exchange efficiency of the system has also been improved. The scale deposited in the sump was collected, and it was found to be in powder form. The main components of the test result were carbonates such...

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Abstract

The invention belongs to the technical field of industrial water treatment, and particularly relates to a method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase. The invention aims to provide a method for relieving the phenomenon of carbonate scales in an industrial circulating water system. The method can reduce the phosphorus-containing wastewater which enters the ambient water body. The technical scheme is as follows: the method comprises the following steps: 1) preparing a bacillus microbial inoculant for secreting extracellular carbonic anhydrase; 2) carrying out refrigerated centrifugation, and salting out to extract a crude enzyme solution containing the carbonic anhydrase; and 3) adding the crude enzyme solution into industrial circulating water with carbonate scales. The method can be used for removing the calcium carbonate scales in the industrial circulating water system, and has the advantages of low cost and no environment pollution.

Description

Technical field [0001] The invention belongs to the technical field of industrial water treatment, and specifically relates to a method for removing calcium carbonate scale by using microbial extracellular carbonic anhydrase. Background technique [0002] In industrial water systems, the presence of calcium carbonate scale affects the water quality of heat exchange and circulating water. After running for a certain period of time, the metal surface of the industrial boiler is easy to form scale. The main reason for the formation of scale is that the boiler feedwater contains a certain amount of calcium and magnesium salts, which undergo physical and chemical changes under the influence of factors such as pressure and temperature inside the boiler. , Generating various types of scale. Circulating cooling water system occupies an important position in industrial production water, accounting for 70%-80% of industrial water consumption. In the process of cooling water recycling, wa...

Claims

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Application Information

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IPC IPC(8): C02F3/00C02F5/08
Inventor 叶姜瑜李大荣石玉竹
Owner CHONGQING UNIV
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