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Multi-channel white light common-channel interference microscopic chromatography system

An orthogonal polarized light and interference microscopy technique, applied in the field of multi-channel white light co-channel interference microscopy tomography system, to achieve the effects of fast calculation speed, enhanced precision and simple production

Active Publication Date: 2014-10-08
GUANGDONG OPTO MEDIC TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] When using the characteristics of the reference light, object and optical common path of the phase contrast microscope and the PSI technology at the same time, it not only makes it possible to measure objects dynamically and quantitatively

Method used

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  • Multi-channel white light common-channel interference microscopic chromatography system
  • Multi-channel white light common-channel interference microscopic chromatography system

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Embodiment Construction

[0031] The system involved in the present invention makes the polarization directions of the reference light and the object light orthogonal, and then passes through the half-wave plate and the 1 / 4 wave plate to obtain interference light fields with phase shifts of 0, 90, 180, and 270 degrees respectively. Three or four photoelectric image sensors record several channels of images simultaneously. Afterwards, the phase information of the object is calculated by an algorithm.

[0032] In the description of the embodiment, the spatial coordinate system in the process of beam transmission is defined as: the direction of beam transmission along the optical axis of the system is the z direction, the x direction is perpendicular to the incident surface and the z direction, the y direction is parallel to the incident surface and perpendicular to the z direction, The three directions x, y, and z form a right-handed coordinate system.

[0033] Such as figure 1 As shown, the phase cont...

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Abstract

The invention discloses a multi-channel white light common-channel interference microscopic chromatography system. The system comprises a phase contrast microscope and a polarization plate, wherein the polarization plate is arranged on the back focal plane of an objective lens of the phase contrast microscope, the polarization direction of the conjugate surface of the polarization plate is orthogonal with the polarization direction of the complementary surface of the polarization plate, and an airspace phase shift interference module is arranged behind the imaging surface of the phase contrast microscope. The airspace phase shift interference module is composed of a phase adjusting device, a beam splitting lens assembly, a quarter-wave plate (15), a polarization beam splitter and an image sensor. According to the multi-channel white light common-channel interference microscopic chromatography system, only an objective lens with a phase plate needs to be used instead on the basis of an existing phase contrast microscope, real-time measurement can be really achieved, an object can be measured quantitatively, and three-dimensional information of the object can be measured in real time in a lossless mode.

Description

technical field [0001] The invention relates to an optical measuring instrument, in particular to a multi-channel white light common-path interference micro-tomography system based on orthogonally polarized light. Background technique [0002] We know that ordinary microscope imaging only allows the amplitude change (brightness) and wavelength change (color) of light to be observed, but most living tiny organisms are colorless and transparent, and when light passes through, the wavelength and amplitude change is not significant, In this way, it is difficult to observe clearly under bright field microscope inspection. In order to overcome this difficulty, measures such as dyeing have been adopted to change the color and brightness of the object to be inspected. However, this method is mostly used in a non-living state, and the effect is not particularly ideal when applied to a living body. Of course, the aperture diaphragm of the condenser is narrowed to increase the contra...

Claims

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Application Information

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IPC IPC(8): G01B9/04G01B11/24G02B21/00
Inventor 王翰林
Owner GUANGDONG OPTO MEDIC TECH CO LTD
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