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Method for detecting human immune globulin E with concentration of 0.5-10[mu]g/mL

A human immunoglobulin, concentration technology, applied in the field of biomedical engineering, can solve problems such as radiation, complicated operation, and inability to meet rapid detection

Active Publication Date: 2014-10-08
GUILIN UNIV OF ELECTRONIC TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Among them, radioimmunoassay has radiation and is not safe; other detection methods are complicated to operate and have low sensitivity, which makes it difficult to realize early diagnosis and research of diseases, and cannot meet the needs of rapid detection.

Method used

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  • Method for detecting human immune globulin E with concentration of 0.5-10[mu]g/mL

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Embodiment 1

[0028] The steps of the present invention to detect hIgE are as follows:

[0029] (1) The carbon nanotube micro-cantilever is placed in a solution containing a nucleic acid aptamer that has a specific recognition effect on hIgE, and the nucleic acid aptamer is modified on the carbon nanotube through a hydrophobic interaction by ultrasonic treatment, forming a detection probe comprising hIgE nucleic acid aptamer;

[0030] (2) Drop the sample to be tested onto the carbon nanotube microcantilever modified with nucleic acid aptamers, and incubate at room temperature for 15 minutes, so that the nucleic acid aptamer on the biosensing interface and the hIgE in the sample to be tested A specific recognition reaction occurs to form a complex;

[0031] (3) The mass size of the formed complex is positively correlated with the concentration of hIgE in the sample to be tested.

[0032] (4) The formed complex produces a mass effect on the micro-cantilever beam, and the detection of hIgE i...

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Abstract

A method for detecting human immune globulin E with the concentration of 0.5-10[mu]g / mL is realized by constructing a carbon nanotube micro-cantilever biosensor. The biosensor comprises a support, a substrate material, a carbon nanotube and a pick-up circuit, and the carbon nanotube is modified with a layer of nucleic acid aptamers through a hydrophobic effect. The method comprises the following steps: a detection probe containing an hIgE nucleic acid aptamer is made on a carbon nanotube micro-cantilever, the detection probe is put in a sample to be detected during detection, hIgE in the sample to be detected and the nucleic acid aptamer on the detection probe form a compound and are attached to the micro-cantilever through a specific reaction; and a micro-cantilever deflection displacement or resonant frequency change caused by the mass change of the compound on the micro-cantilever is positively related to the concentration of hIgE in the sample to be detected in order to realize hIgE detection.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for detecting hIgE with a micro-cantilever beam biosensor. technical background [0002] Human immunoglobulin E (hIgE) is a common tumor marker. Currently, there are many methods for detecting hIgE, including radioimmunoassay, enzyme-linked immunoassay, and fluorescence immunoassay. Among them, the radioimmunoassay method has radiation and is not safe; other detection methods are complicated to operate and have low sensitivity, making it difficult to realize the diagnosis and research of early diseases, and cannot meet the needs of rapid detection. "Nanomechanical microcantilever oprerated in vibratin modes with use of RNA aptamer as a receptor molecules for label-free detection of HCV helicase", Kyo Seon Hwang et al., Biosensors and Bioelectronics, Vol. 23, pp. 459-465, 20070602, published A method for detecting HCV helicase by using nucleic acid aptamer as ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N5/00
CPCG01N5/00G01N33/6854
Inventor 黄勇梁晋涛李桂银周治德黄国银管明源马龙飞
Owner GUILIN UNIV OF ELECTRONIC TECH
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