Mongolian medicine for treating painful subacute thyroiditis and preparation method thereof
A technology of submethoditis and Mongolian medicine, applied in the medical field
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[0030] Preparation of Reference Substance Solution Accurately weigh 20.75mg of rutin reference substance, place it in a 100mL volumetric flask, dissolve and dilute to the mark with 75% ethanol, and shake well. A rutin standard solution of 0.2075 mg / mL was prepared as a stock solution.
[0031] Preparation of standard curve Precisely draw 0.0, 1.0, 2.0, 3.0, 4.0, 5.0mL rutin stock solution (0.2075mg / mL), respectively, put them in 25mL volumetric flasks, add 75% ethanol to 7mL, then add 5% NaNO 2 Solution 1mL, stand for 6min, add 10% Al(NO 3 ) 3Solution 1mL, shake well and let it stand for 6min, add 10mL of 4% NaOH solution, and finally use 75% ethanol solution to make the volume to the mark, seal it tightly, shake well, let it stand for 15min, take the corresponding blank as a reference, and use ultraviolet-visible spectrometry The absorbance was measured at 510nm by photometric method (Appendix VA of "Chinese Pharmacopoeia" 2010 Edition). With the absorbance as the ordinate...
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