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Method for utilizing liquid chromatography tandem mass spectrometry to measure geraniol in flavors and fragrances

A flavor and fragrance, liquid chromatography technology is applied in the field of rapid determination of geraniol in flavors and fragrances by liquid chromatography-tandem mass spectrometry, and achieves the effects of high sensitivity, good linear correlation and good repeatability

Active Publication Date: 2014-10-15
CHINA TOBACCO FUJIAN IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

LC-MS / MS method for detection of geraniol in flavors and fragrances has not been reported

Method used

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  • Method for utilizing liquid chromatography tandem mass spectrometry to measure geraniol in flavors and fragrances
  • Method for utilizing liquid chromatography tandem mass spectrometry to measure geraniol in flavors and fragrances
  • Method for utilizing liquid chromatography tandem mass spectrometry to measure geraniol in flavors and fragrances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] In this embodiment, the content of geraniol in the flavor and fragrance for tobacco is detected, and the detection method is as follows, and the flow chart of the detection method is as follows: figure 1 shown.

[0046](1) Weigh 0.1g of geraniol (analytical pure), accurate to 0.0001g, dissolve it in methanol, transfer it to a 1000mL volumetric flask, and set it to the standard stock solution; pipette 0.01mL, 0.05mL, 0.1 mL, 0.5mL, 1.0mL standard stock solution into a 100mL volumetric flask, dilute to volume with 10% (v / v) methanol aqueous solution, then the concentration of geraniol is 0.01μg / mL, 0.05μg / mL, 0.1μg / mL mL, 0.5 μg / mL, 1.0 μg / mL to obtain a series of standard solutions of geraniol. Store the standard solution in the dark at 4°C, store it at room temperature when taking it, and use it after reaching room temperature. Make markings once a week;

[0047] (2) Preparation of sample solution: Weigh about 0.1 g of the tobacco flavor and fragrance sample to be te...

Embodiment 2

[0062] In this embodiment, the repeatability and standard addition recovery of the method of the present invention are tested, and the detection method is as follows.

[0063] The recovery rate of the method of the present invention was determined by the standard addition method. Weigh 0.1 g of the flavor and fragrance sample for tobacco to be tested (the sample is the same as the flavor and fragrance sample used in step (2) of Example 1), in three parts, add 0.01 mL, 0.02 mL, and 0.05 mL of Example 1 to it respectively. Prepare the geraniol standard stock solution in step (1), then prepare the standard sample solution according to the preparation method in Example 1 step (2) "Preparation of sample solution", each sample is measured 6 times, chromatographic and mass spectrometry The conditions of analysis are the same as in Example 1, and the recovery rate of standard addition of geraniol in the flavor and fragrance for tobacco of this method is calculated according to the ana...

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Abstract

The invention relates to a method for detecting geraniol in flavors and fragrances. According to the method, geraniol is measured by adopting liquid chromatography tandem mass spectrometry, and quantification is performed by adopting an external standard method. The detecting method has the advantages of short detection time, easiness and convenience in operation, high sensitivity and recovery rate and good repeatability. Due to the chromatographic conditions of the method, the chromatographic peak of geraniol is separated from the impurity chromatographic peak well, the method has a good linear range, the detection limit is 0.001 microgram / mL, the recovery rate is 90.8%-98.3%, and the relative standard deviation (RSD) is smaller than 5% (n=6).

Description

technical field [0001] The invention belongs to the technical field of chemical detection and relates to a method for determining geraniol, in particular to a method for rapidly determining geraniol in flavors and fragrances by means of liquid chromatography-tandem mass spectrometry. Background technique [0002] Geraniol (Geraniol, trans-3,7-dimethyl-2,6-octadien-1-ol), also known as geraniol and geraniol, is a monoterpene alcohol that naturally exists in Palmarosa oil (content about 80%), geranium oil, jasmine oil and other more than 250 kinds of plants have a rose-like fragrance and are commonly used as the main fragrance agent for spices. They are widely used in medicine, tobacco, food ingredients and other fields. It plays an important role in the aroma of cigarettes and is the most common flavor used in tobacco. It can increase the rose aroma and sweet aroma of cigarettes, and if it is too much, it will produce soap aroma and floral aroma. [0003] At present, the det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 邓其馨黄朝章张建平苏明亮刘江生谢卫
Owner CHINA TOBACCO FUJIAN IND
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