Beautyberry leaf injection with astringency and hemostasis effects and preparation method thereof

[0034] Example 1 The preparation steps of Zizhucao injection with astringent and hemostatic effects are as follows:

[0035] (1) Take the purple grass powder, extract twice with water, boil for 1 hour each time, combine the two filtrates, concentrate to a thick paste, add ethanol, stir well, wash, separate on a cloth funnel, and discard the ethanol The mother liquor is precipitated and dried below 60°C to obtain the crude water extract dry powder;

[0036] (2) Take the crude dry powder obtained in step (1), dissolve it with a small amount of water for injection, add lead acetate, stir well, place in the refrigerator overnight, separate the precipitate to obtain a pale yellow solution, add 50% sulfuric acid dropwise to adjust pH1 to remove lead, filter Obtain a light yellow solution, add 2N sodium carbonate dropwise to make the pH 6.5, add 5 times the amount of 65% ethanol to precipitate the precipitate, discard the mother liquor, and dry below 60℃ to obtain a white or off-white powder;

[0037] (3) Put Panax notoginseng in a casserole, add water and decoct twice, the first time is boiled for 1 hour, the second time is boiled for 0.5 hours, the two filtrates are combined, concentrated to a thick paste, and dried;

[0038] (4) Take the resultant from steps (2)-(3), add water for injection to dissolve according to the prescription amount, adjust the pH to 6.5, treat once with activated carbon, filter by suction, add water for injection to the full amount, control the obtained and have both convergence and hemostasis effect The main ingredients per 100ml of the purple grass injection: 2g of purple grass water extract, 3g of panax notoginseng water extract, finely filtered with a glass funnel, divided into 2 ml ampoules, sterilized for 30 minutes.

[0039] Usage: intramuscular injection, 2-4ml once.

[0040] Example 2 The preparation steps of Zizhucao injection with astringent and hemostatic effects are as follows:

[0041] (1) Take the purple grass powder, extract twice with water, boil for 2 hours each time, combine the two filtrates, concentrate to a thick paste, add ethanol, stir well, wash, separate on a cloth funnel, and discard the ethanol The mother liquor is precipitated and dried below 60°C to obtain the crude water extract dry powder;

[0042] (2) Take the crude dry powder obtained in step (1), dissolve it with a small amount of water for injection, add lead acetate, stir well, place in the refrigerator overnight, separate the precipitate to obtain a pale yellow solution, add 50% sulfuric acid dropwise to adjust pH2 to remove lead, filter Obtain a light yellow solution, add 2N sodium carbonate dropwise to make the pH 6.5, add 5 times the amount of 65% ethanol to precipitate the precipitate, discard the mother liquor, and dry below 60℃ to obtain a white or off-white powder;

[0043] (3) Put Panax notoginseng in a casserole, add water and decoct twice, the first time is boiled for 3 hours, the second time is boiled for 1.5 hours, the two filtrates are combined, concentrated to a thick paste, and dried;

[0044] (4) Take the resultant from steps (2)-(3), add water for injection to dissolve according to the prescription amount, adjust the pH to 6.5, treat once with activated carbon, filter by suction, add water for injection to the full amount, control the obtained and have both convergence and hemostasis effect The main components per 100ml of the Echinacea injection: 4g of Echinacea water extract, 6g of Panax notoginseng water extract, finely filtered with a glass funnel, divided into 10 ml ampoules, sterilized for 30 minutes.

[0045] Usage: intravenous drip, 20-40ml once, diluted with 250-500ml of 5% glucose injection, and then applied.

[0046] Typical case one: Ma XX, male, 2 years old, from Dongying City, Shandong Province. Frequent vomiting, unsteady walking, and constant wrestling, began to exhibit abnormal behaviors. After examination, it was discovered that a tumor had grown on the back of the brain. The family didn't care about the symptoms at first, thinking it was because the child had just learned to walk and was not used to it. Unexpectedly, I often vomited and fell easily when I walked, but my family thought it was normal and did not take the child to the hospital for examination. After more than a month, he was obviously thin, and the parents hurriedly took the child to the hospital for examination. Head CT showed that there was a tumor on the back of the skull, which was a "posterior fossa tumor". So he underwent surgery at the city hospital. According to the director of the Department of Neurosurgery of Children's Hospital, the tumor is located in the cerebellar vermis, pressing the brain stem forward, invading the fourth ventricle downward, and extending down to the foramen magnum, causing hydrocephalus.

[0047] During the operation, the hospital used the Echinacea injection obtained in Example 2 with astringent and hemostatic effects. On the day of the operation, 40ml of Echinacea injection was injected intravenously, diluted with 300ml of 5% glucose injection, and then used. Bleeding during the operation Not much. In order to prevent the possibility of continued bleeding, the doctor chose to continue intravenous infusion of 20 ml of the hemostatic Echinacea injection obtained in Example 2 and diluted with 200 ml of 5% glucose injection for use. The bleeding was suppressed and no bleeding was found. So far, he has been discharged from the hospital smoothly.

[0048] Example 3 The preparation steps of Zizhucao injection with astringent and hemostatic effects are as follows:

[0049] (1) Take the purple grass powder, extract twice with water, boil for 1.2 hours each time, combine the two filtrates, concentrate to a thick paste, add ethanol, stir well, wash, separate on a cloth funnel, and discard the ethanol The mother liquor is precipitated and dried below 60°C to obtain the crude water extract dry powder;

[0050] (2) Take the crude dry powder obtained in step (1), dissolve it with a small amount of water for injection, add lead acetate, stir well, place in the refrigerator overnight, separate the precipitate to obtain a pale yellow solution, add 50% sulfuric acid dropwise to adjust pH1 to remove lead, filter Obtain a light yellow solution, add 2N sodium carbonate dropwise to make the pH 6.5, add 5 times the amount of 65% ethanol to precipitate the precipitate, discard the mother liquor, and dry below 60℃ to obtain a white or off-white powder;

[0051] (3) Put Panax notoginseng in a casserole, add water to decoct twice, boil for 1.5 hours for the first time, and boil for 0.8 hours for the second time, combine the two filtrates, concentrate to a thick paste, and dry;

[0052] (4) Take the resultant from steps (2)-(3), add water for injection to dissolve according to the prescription amount, adjust the pH to 6.5, treat once with activated carbon, filter by suction, add water for injection to the full amount, control the obtained and have both convergence and hemostasis effect The main ingredients per 100ml of the Echinacea injection: 2.5g of Echinacea water extract, 4g of Panax notoginseng water extract, finely filtered with a glass funnel, divided into 2ml ampoules, sterilized for 30 minutes.

[0053] Usage: intramuscular injection, 2-4ml once.