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Anticancer drug

A drug and a technology for preparing drugs, which are applied in the field of J1-001 compound as an anticancer drug, can solve the problems that tumor cells affect the clinical chemotherapy effect and cannot fundamentally cure tumors, and achieve good industrial applicability

Active Publication Date: 2014-11-12
武汉合生科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy, radiotherapy, and biological immunotherapy are often used clinically to kill tumor cells, but they cannot fundamentally cure tumors, mostly because the drug resistance of tumor cells seriously affects the clinical chemotherapy effect (according to estimates by the American Cancer Society, 90% The above cancer patients died of different degrees of tumor cell drug resistance)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Inhibitory effect of J1-001 sodium salt and paclitaxel on the proliferation of HO-8910 cells

[0037] HO-8910 (ovarian cancer cells) in the logarithmic growth phase were taken respectively, digested with 0.25%-EDTA trypsin and prepared at a concentration of 5×10 4 cells / mL of cell suspension. The cell suspension was inoculated into a 96-well cell culture plate, 200 μL per well; the blank group was the group in which 200 μL of cell-free medium was added to each well. The above-mentioned 96-well cell culture plate inoculated with cells was placed in a cell culture incubator and incubated overnight to allow the cells to adhere to the wall. The sodium salt of sample J1-001 and the existing anticancer drug paclitaxel were dissolved in DMSO respectively, prepared as a 100mmol / L stock solution, and then diluted into sample solutions with different concentrations (the final concentration of DMSO was less than 0.1%) with the corresponding medium before use. 200 μL o...

Embodiment 2

[0043] Example 2: The inhibitory effect of J1-001 sodium salt on the proliferation of various cell lines in vitro

[0044] The experimental method is the same as in Example 1, except that the selected cell lines are MCF-7 (human breast cancer cells), Bel-7402 (human liver cancer cells), HepG2 (human liver cancer cells), NCI-H460 (human lung cancer cells) cells), SGC-7901 (human gastric cancer cells) and HO-8910 (human ovarian cancer cells).

[0045] Depend on figure 1 The results shown show that J1-001 sodium salt has different degrees of inhibitory effects on the proliferation of MCF-8, Bel-7402, HepG2, NCI-H460, SGC-7901 and HO-8910 cells in vitro, among which, MCF-7 has inhibitory effects on J1 -001 sodium salt is the most sensitive, showing the lowest IC 50 value. It can be seen that J1-001 sodium salt has a good inhibitory effect on the proliferation of the above tumor cells.

Embodiment 3

[0046] Example 3: Inhibitory effect of J1-001 sodium salt on the proliferation of drug-resistant tumor cells in vitro

[0047] The experimental method is the same as in Example 1, except that the selected cells are Bel-7402 (human liver cancer cells); Bel-7402 / 5-Fu (human liver cancer drug-resistant cells).

[0048] Table 2. IC of J1-001 sodium salt, paclitaxel and doxorubicin on Bel-7402 and Bel-7402 / 5-Fu 50 value

[0049]

[0050] From the experimental results shown in Table 2, it can be seen that J1-001 sodium salt has a good inhibitory effect on the cell proliferation of drug-resistant Bel-7402 / 5-Fu and non-drug-resistant tumor cell line Bel-7402, and has a good inhibitory effect on the non-drug-resistant tumor cell line The cell line Bel-7402's activity of inhibiting cell proliferation is similar to that of paclitaxel, and better than that of adriamycin; it is worth mentioning that J1-001 sodium salt is resistant to Bel-7402 / 5-Fu tumor cell line showed the lowest IC ...

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Abstract

Provided in the present invention is the use of a J1-001 compound as an anti-cancer drug, including the use of the compound shown in formula I or pharmaceutical acceptable salts thereof in the preparation of a drug for inhibiting the proliferation of tumour cells.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of J1-001 compound as an anticancer drug. Background technique [0002] At present, cancer has become one of the main causes of human death. Chemotherapy, radiotherapy, and biological immunotherapy are often used clinically to kill tumor cells, but they cannot fundamentally cure tumors, mostly because the drug resistance of tumor cells seriously affects the clinical chemotherapy effect (according to estimates by the American Cancer Society, 90% The above tumor patients died of different degrees of tumor cell drug resistance). Therefore, finding a drug with high efficiency and low toxicity, which is sensitive to drug-resistant tumor cells, has a very positive effect on the treatment of cancer. Contents of the invention [0003] The present invention aims at solving one of the above technical problems at least to a certain extent or at least providing a useful commercial choi...

Claims

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Application Information

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IPC IPC(8): A61K31/35A61P35/00A61P35/02A61K31/704A61K31/337
CPCA61K31/351A61P35/00A61P35/02
Inventor 刘天罡
Owner 武汉合生科技有限公司
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