Feed additive for preventing dairy cow mastitis and brucellosis and preparation method thereof
A technology for brucellosis and cow mastitis, applied in the field of feed, can solve the problems of antibiotic residues, veterinary drug residues, endocrine dysfunction, and increase of drug-resistant strains, so as to avoid adverse effects, improve economic benefits, and improve health status Effect
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Embodiment 1
[0034] Embodiment 1: the preparation of yeast culture
[0035] 1) Seed solution preparation: Candida utilis ( Candida utilis ) CGMCC NO.7691 was inoculated into the liquid seed medium to obtain the seed liquid. The liquid seed medium contained: molasses 10-20g / L, peptone 10-20g / L, sodium chloride 5-25g / L, pH 5.5-7.0, the seed culture conditions are: shaker culture, rotation speed 150-200r / min, temperature 28-32 ℃, culture time 18-24h;
[0036] 2) Liquid aerobic fermentation: Inoculate the seed liquid into the liquid fermentation medium of the fermenter, scale up the culture, and carry out aerobic fermentation to obtain a liquid strain solution. The liquid fermentation medium contains: molasses 40-80g / L , peptone 30-50g / L, sodium chloride 5-25 g / L, MgSO4•7H2O 1-5 g / L, pH 5.5-7.0, liquid aerobic fermentation conditions: inoculum size 10-20%, ventilation volume 1 -3L / min, stirring speed 200-400r / min, using the fermenter pH automatic feedback adjustment system to control the pH ...
Embodiment 2
[0044] Embodiment 2: be used for the preparation of the feed additive of prevention milk cow mastitis and brucellosis
[0045] (1) Weigh the raw materials according to 50% yeast culture, 8% cecropin, 10% medlar, 10% jujube, 10% safflower, 8% white peony root and 4% montmorillonite;
[0046] (2) Mix the weighed raw materials, crush them to about 40-60 mesh, and mix well to obtain the feed additive for preventing mastitis and brucellosis in dairy cows of the present invention.
Embodiment 3
[0047] Embodiment 3: be used for the preparation of the feed additive of prevention milk cow mastitis and brucellosis
[0048] (1) Weigh the raw materials according to 60% of yeast culture, 6% of cecropin, 6% of medlar, 8% of jujube, 6% of safflower, 10% of white peony and 4% of montmorillonite;
[0049] (2) Mix the weighed raw materials, crush them to about 40-60 mesh, and mix well to obtain the feed additive for preventing mastitis and brucellosis in dairy cows of the present invention.
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