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Denture care solution containing enzyme and pericarpium citri reticulatae extract and preparation method thereof

A technology of tangerine peel extract and care solution, which is applied in the field of daily chemicals, and can solve the problems that there is no care solution for removing dental plaque, etc., and achieves remarkable effects and the effect of inhibiting aerobic demand

Active Publication Date: 2014-12-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many denture care products, and more disinfectant ingredients are added, but there is no care solution for removing dental plaque

Method used

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  • Denture care solution containing enzyme and pericarpium citri reticulatae extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) The preparation method of tangerine peel extract:

[0032] In 30mL pre-cooled ethanol solution (mass percentage concentration is 80%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 12000rpm, dispersion liquid homogeneous 5min is in high-speed homogenizer; Adopt centrifuge to Centrifuge at 3000rpm for 3min and collect the supernatant;

[0033] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 80%) to continue homogenization (rotating speed is 12000rpm, homogenization time is 5min), centrifugation (centrifugal speed is 3000rpm, centrifugal time is 3min) to the precipitate after centrifugation, take Supernatant, so repeated 5 times, combined supernatant.

[0034] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and stored at -40°C for later us...

Embodiment 2

[0039] (1) The preparation method of tangerine peel extract:

[0040] In 30mL pre-cooled ethanol solution (mass percentage concentration is 90%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 12000rpm, in high-speed homogenizer, dispersion liquid homogeneous 8min; Adopt centrifuge to Centrifuge at 4000rpm for 5min to collect the supernatant;

[0041] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 90%) to the precipitate after centrifugation to continue homogenization (rotating speed is 11000rpm, homogenization time is 8min), centrifugal (centrifugal speed is 4000rpm, centrifugal time is 5min), take Supernatant, so repeated 6 times, combined supernatant.

[0042] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the obtained concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and stored at -40°C after sub...

Embodiment 3

[0047] (1) The preparation method of tangerine peel extract:

[0048] In 30mL pre-cooled ethanol solution (mass percentage concentration is 100%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 10000rpm, dispersion liquid is homogeneous 10min in high-speed homogenizer; Adopt centrifuge to Centrifuge at a speed of 5000rpm for 3min, and collect the supernatant;

[0049] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 100%) to continue homogenization (rotating speed is 10000rpm, homogenization time is 10min), centrifugation (centrifugal speed is 5000rpm, centrifugal time is 3min) to the precipitate after centrifugation, take Supernatant, so repeated 3 times, combined supernatant.

[0050] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the obtained concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and sto...

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Abstract

The invention belongs to the technical field of daily chemicals and discloses a denture care solution containing enzyme and pericarpium citri reticulatae extract and a preparation method thereof. 100mL of the denture care solution is prepared from the following components: 2.5-10mL of pericarpium citri reticulatae extract, 0.25-10mg of alpha-glucanase, 0.25-10mg of beta-glucanase, 0.25-10mg of alpha-amylase, 1g of edible salt and the balance of water. The denture care solution prepared by the invention has a remarkable dental plaque cleaning effect, is capable of effectively inhibiting growth of aerobic and anaerobic bacteria, and especially has a remarkable inhibiting effect on proliferation of streptococcus. Meanwhile, since the denture care solution is safe, non-toxic and non-irritative, artificial teeth can be directly used without being washed after being soaked in the denture care solution.

Description

technical field [0001] The invention belongs to the technical field of daily chemicals, and in particular relates to a denture care solution containing enzyme and tangerine peel extract and a preparation method thereof. Background technique [0002] Correct and effective cleaning and care of dentures can prolong the service life of dentures, avoid damage and deformation of dentures caused by the proliferation of cariogenic bacteria on dentures, and even cause caries or gingivitis of the surrounding teeth that are in contact with dentures. Dental plaque is a biofilm community formed by oral flora, especially Streptococcus mutans, which is closely related to dental caries, on teeth and dentures. It is rich in polysaccharides such as glucan and fructan. Helps the growth of cariogenic bacteria in the oral cavity, causing caries, ulcers or other oral diseases, and even increases the risk of other systemic diseases. At present, there are many denture care products that add more d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K8/66A61Q11/02
Inventor 陈谷王宏
Owner SOUTH CHINA UNIV OF TECH
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