A denture care solution containing enzyme and tangerine peel extract and preparation method thereof
A technology of tangerine peel extract and nursing solution, which is applied in the field of daily chemicals, can solve the problems such as the nursing solution that has not yet removed dental plaque, and achieves remarkable effect and the effect of inhibiting oxygen demand.
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Embodiment 1
[0031] (1) The preparation method of tangerine peel extract:
[0032] In 30mL pre-cooled ethanol solution (mass percentage concentration is 80%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 12000rpm, dispersion liquid homogeneous 5min is in high-speed homogenizer; Adopt centrifuge to Centrifuge at 3000rpm for 3min and collect the supernatant;
[0033] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 80%) to continue homogenization (rotating speed is 12000rpm, homogenization time is 5min), centrifugation (centrifugal speed is 3000rpm, centrifugal time is 3min) to the precipitate after centrifugation, take Supernatant, so repeated 5 times, combined supernatant.
[0034] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and stored at -40°C for later us...
Embodiment 2
[0039] (1) The preparation method of tangerine peel extract:
[0040] In 30mL pre-cooled ethanol solution (mass percentage concentration is 90%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 12000rpm, in high-speed homogenizer, dispersion liquid homogeneous 8min; Adopt centrifuge to Centrifuge at 4000rpm for 5min to collect the supernatant;
[0041] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 90%) to the precipitate after centrifugation to continue homogenization (rotating speed is 11000rpm, homogenization time is 8min), centrifugal (centrifugal speed is 4000rpm, centrifugal time is 5min), take Supernatant, so repeated 6 times, combined supernatant.
[0042] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the obtained concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and stored at -40°C after sub...
Embodiment 3
[0047] (1) The preparation method of tangerine peel extract:
[0048] In 30mL pre-cooled ethanol solution (mass percentage concentration is 100%), add 2.0g dry tangerine peel powder, obtain dispersion liquid; With the rotating speed of 10000rpm, dispersion liquid is homogeneous 10min in high-speed homogenizer; Adopt centrifuge to Centrifuge at a speed of 5000rpm for 3min, and collect the supernatant;
[0049] Add 30mL pre-cooled ethanol solution (mass percentage concentration is 100%) to continue homogenization (rotating speed is 10000rpm, homogenization time is 10min), centrifugation (centrifugal speed is 5000rpm, centrifugal time is 3min) to the precipitate after centrifugation, take Supernatant, so repeated 3 times, combined supernatant.
[0050] Concentrate the combined supernatant in a rotary evaporator until there is no flowing liquid in the obtained concentrate to obtain the orange peel concentrate, which is dissolved in 10 mL of reverse osmosis purified water, and sto...
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