Streptomyces clavuligerus and use method of streptomyces clavuligerus in melanin production
A technology of Streptomyces coryneformis and application method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as lack of microbial strains, etc., and achieve the effects of convenient operation, simple method and stable performance.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] Embodiment 1, strain isolation and identification:
[0037] (1) Strain isolation:
[0038] Soil samples were collected from different locations in Jiaxing Park. When sampling, the soil at a depth of 5cm to 20cm below the soil surface was selected, and brought back to the laboratory in a sterile paper bag; after air-drying and grinding, 1g of the soil sample was added to 99mL of deionized water , to make a soil suspension, and successively diluted to 10 -5 、10 -6 ; take 10 -5 、10 -6 Spread 0.1mL of the dilution solution on the screening medium, culture at 37°C, pick out the colonies with early melanin production time and dark color, and then separate and purify them by streaking multiple times to obtain a single colony;
[0039]The formula of the screening medium is: beef extract 3g, peptone 10g, NaCl 5g, agar 15-20g, pH7.0-7.2, water 1000mL;
[0040] (2) Morphological identification:
[0041] The selected melanin-producing single colony was inoculated in Gaoshi Sy...
Embodiment 2
[0047] Embodiment 2, bacterial strain fermentation produces melanin:
[0048] (1) Pick colonies from the test tubes in which the strains are preserved for strain activation, and cultivate them at 37°C for 48 hours; the formula for the activation medium is:
[0049] (2) pick a single bacterium colony on the activated plate and transfer it to the seed culture solution for proliferation and culture, cultivate it at 37°C for 48 hours, and the rotation speed is 200r / min; the formula of the seed culture solution is (g / L): soluble starch 2g, KNO 3 0.1g, K 2 HP0 4 0.05g, NaCl0.05g, MgSO 4 ·7H 2 O0.05g, FeS0 4 ·7H 2 O0.01g, pH7.2-7.4.
[0050] (3) Inoculate the seed liquid into the fermentation culture liquid for fermentation and culture, and cultivate it on a shaking table at 28° C. for 7 days at a rotation speed of 200 r / min until the fermentation liquid is black;
[0051] (4) Take the black fermented liquid and centrifuge at 10000r / min, take its supernatant, concentrate it b...
Embodiment 3
[0052] Example 3, obtaining melanin basic data:
[0053] A series of tests were carried out on the melanin sample prepared in Example 2 to obtain the basic data of the melanin:
[0054] (1) Structural characterization:
[0055] The melanin was subjected to ultraviolet-visible full-band scanning and infrared spectrum analysis, and the results were as follows: Figure 4 and Figure 5 shown;
[0056] Depend on image 3 It can be seen that the melanin has a strong absorption peak at about 240nm, and there is no characteristic peak in the visible light region, and the absorbance decreases with the increase of the wavelength. The results are consistent with the literature (Zheng Chenna, Fang Baishan, Luo Juxiang, etc. 4. Extraction and physicochemical properties of melanin. The melanin produced by streptomycin G-HD-4 in Huaqiao University (Natural Science Edition), 2009, 30(3):292-296.) is similar;
[0057] Depend on Figure 5 It can be seen that the infrared spectrum of the m...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com