30 results about "Streptomyces clavuligerus" patented technology

The present invention relates to a process of producing a monoterpene and / or derivatives thereof. The process comprises the steps of: a) providing a host microorganism genetically engineered to express a bacterial monoterpene synthase (mTS); and b) contacting geranyl pyrophosphate (GPP) with said bacterial mTS to produce said monoterpene and / or derivatives thereof. The present invention also relates to a microorganism for use in producing a monoterpene and / or derivatives thereof and a recombinant microor-ganism adapted to conduct the step of converting geranyl pyrophosphate (GPP) into a monoterpene and / or derivatives thereof by ex-pression of a bacterial mTS. It was shown to produce 1,8 cineole using 1,8 cineole synthase and to produce linalool using linalool synthase, both from Streptomyces clavuligerus.

The invention discloses a corn steep liquor fermentation-enzyme catalysis method, which is used to synthesize phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Streptomyces clavuligerus with an expandase gene is used to make strain transformation to corn steep liquor to ensure that the corn steep liquor is made into fermentation ring enlargement in a culture medium and is reacted with phenylacetic acid added into the culture medium to generate the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Water solution of the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid is obtainedthrough organic solvent extraction and water back extraction. Acetyl transferase and nutrition content are added into the water solution to form an enzyme reaction culture medium and finish a biochemical reaction under appropriate condition, and the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid can be produced after side chains are removed.

The invention belongs to the field of microbial engineering, provides a superior streptomyces clavuligerus strain that is used for clavulanic acid production, a strain preparation method and application of the strain, and concretely relates to a method, which obtains high expression of key enzyme in the arginine synthesizing process by increasing the copy number of clavulanic acid synthesizing precursor of the streptomyces clavuligerus strain that is key enzyme gene argG in the arginine synthesizing process, and then obtains the streptomyces clavuligerus strain LNSC-2 with high yield of clavulanic acid. Compared with the original strains, the superior streptomyces clavuligerus strain that is provided by the invention can improve the yield of the zymolytic clavulanic acid production by over 20 percent.

The invention discloses a genetically engineered bacterium for converting cephalosporin C and a preparation method thereof. The genetically engineered bacterium integrates an expression cassette of cmcJ gene into the genome of a wild strain of Streptomyces clavuligerus (ATCC27064). It is verified by cephalosporin C conversion that the genetically engineered bacterium is advantaged in that the conversion rate from cephalosporin C to hydroxy cephalosporin C is improved from 57.0% of a wild strain to 67.4% of a recombinant mutant, and the conversion rate of methoxy cephalosporin C is improved from 43.1% of the wild strain to 51.9% of the recombinant mutant.

The invention discloses streptomyces clavuligerus and a use method of streptomyces clavuligerus in melanin production. The use method comprises the following steps of carrying out strain activation on streptomyces clavuligerus preserved by refrigeration, then carrying out propagation culture, inoculating a fermentation culture liquid with the strain liquid, carrying out fermentation culture to obtain a fermentation broth, separating a melanin product from the fermentation broth, and drying the melanin product. The strain culture liquid is inoculated with streptomyces clavuligerus and the streptomyces clavuligerus strain is subjected to propagation culture so that the strain liquid is obtained, the strain liquid is transferred to the fermentation culture liquid and is subjected to fermentation culture so that the fermentation broth is obtained, and the melanin product is separated from the fermentation broth and then is dried. The use method has simple processes, can be operated conveniently and can rich melanin production bacterium strain resources. The melanin product has stable performances and has the highest yield of 0.85g / L. The use method provides an efficient and stable method for melanin production and fills in gap of use of microbial strain in melanin production.