30 results about "Streptomyces clavuligerus" patented technology

A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I):In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of beta lactam antibiotics against many beta-lactamase producing bacteria.

The invention discloses genetic engineering bacteria for producing high-purity cephalosporin and application of the genetic engineering bacteria. The genetic engineering bacteria is obtained by introducing the following fusion gene into host bacteria: the fusion gene formed by a DNA fragment shown by nucleotides from the 7th site to the 1194th site in a sequence 1 in a sequence table and an encoding gene of a protein cefF. One of the obtained genetic engineering bacteria is called Acremonium chrysogenum cffF genetic engineering bacterium with the preserving number of CGMCC No.4515. In the invention, a Streptomyces clavuligerus sourced gene cefF with an Acremonium chrysogenum promoter PpcbC is transferred to the Acremonium chrysogenum so that the gene cefF is transcribed and expressed in vivo to enhance a catalytic reaction in which DAOC (Deacetoxycephalosporin C) is hydroxylated into DAC (Deacetylcephalosporin C), thereby reducing the content of the DAOC in a finished product CPC (Cephalosporin C), and improving the purity of industrial products.

The invention discloses an engineered strain of streptomyces clavuligerus, and a preparation method and application thereof. Through treating streptomyces clavuligerus by using an ultraviolet mutagenesis technology firstly, a mutant strain B372 is obtained, wherein the clavulanic acid yield of the mutant strain B372 is 1.9 times as large as that of an original strain; and then, through taking the mutant strain as a starting strain, a mutant strain S.clavuligeruslat:: Delta subjected to lat gene knockout is built by using PCR-TARGETING. Studies show that after ultraviolet mutagenesis and lat gene knockout, the clavulanic acid yield of the streptomyces clavuligerus is significantly increased and is 2.3 times as large as that of the original strain. The clavulanic acid yield of the engineered strain disclosed by the invention is significantly increased, and the engineered strain does not contain any resistance makers, therefore, the engineered strain is extremely conveniently used for further genetic engineering reconstruction; and through continuous passage, the clavulanic acid yield is relatively stable and good in repeatability.

The invention belongs to the field of microbial engineering, provides a superior streptomyces clavuligerus strain that is used for clavulanic acid production, a strain preparation method and application of the strain, and concretely relates to a method, which obtains high expression of key enzyme in the arginine synthesizing process by increasing the copy number of clavulanic acid synthesizing precursor of the streptomyces clavuligerus strain that is key enzyme gene argG in the arginine synthesizing process, and then obtains the streptomyces clavuligerus strain LNSC-2 with high yield of clavulanic acid. Compared with the original strains, the superior streptomyces clavuligerus strain that is provided by the invention can improve the yield of the zymolytic clavulanic acid production by over 20 percent.

The invention discloses a streptomyces clavuligerus for high-yield clavulanic acid, and application of the streptomyces clavuligerus. The streptomyces clavuligerus for high-yield clavulanic acid is specifically a streptomyces clavuligerus 16CF10 of which the preservation number is CGMCC No.7915 in the China General Microbiological Culture Collection Center. According to the invention, a streptomyces clavuligerus 70116 is taken as a starting strain, is subjected to metabolic engineering modification and is mutated to obtain the streptomyces clavuligerus 16CF10 CGMCC No.7915. Experimental results show that the amount of clavulanic acid produced by the strain cultivated through fermentation reaches 2.25 g/L of fermentation liquor, and is improved by about 51% compared with that the streptomyces clavuligerus 70116 serving as the starting strain, so that the streptomyces clavuligerus 16CF10 CGMCC No.7915 has a broad industrial application prospect.

The invention discloses a high-activity aminoacetylase clavulanate streptomyces clavuligerus strain. The clavulanate streptomyces clavuligerus strain is clavulanate streptomyces B02-246, is preserved in the China general microbiological culture collection center (CGMCC) on October 13, 2015 and has a preservation number of CGMCC NO. 11484. A sequential error-prone PCR method is used for in-vitro mutation of a gene of the aminoacetylase clavulanate, an aminoacetylase clavulanate-less strain is used as a starter, through a conjugational transfer technology, a clavulanate streptomyces gene-containing mutant library is built, a positive mutant is screened, and through fermentation verification and subculture, the clavulanate streptomyces B02-246 with stable genetic characters is obtained. The strain has a clavulanic acid synthesis level 1.3 times higher than that of the original strain B02 and can be used for industrial fermentation production.

The invention discloses a corn steep liquor fermentation-enzyme catalysis method, which is used to synthesize phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Streptomyces clavuligerus with an expandase gene is used to make strain transformation to corn steep liquor to ensure that the corn steep liquor is made into fermentation ring enlargement in a culture medium and is reacted with phenylacetic acid added into the culture medium to generate the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid. Water solution of the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid is obtained through organic solvent extraction and water back extraction. Acetyl transferase and nutrition content are added into the water solution to form an enzyme reaction culture medium and finish a biochemical reaction under appropriate condition, and the phenylacetyl-7-amino-3- desacetoxycephalos-porin acid can be produced after side chains are removed.

The invention belongs to the field of microbial engineering, provides a streptomyces clavuligerus strain LNSC with low oxygen consumption and high fermentation yield, a strain preparation method and application of the strain, and particularly relates to a method. The method utilizes genetic engineering methods, researches the application aspect of vitreoscilla hemoglobin gene (vhb), transfers the gene into the streptomyces clavuligerus culture that is used for clavulanic acid production, obtains high expression of VHB protein, thus improving the production characteristics of the streptomyces clavuligerus, and lowering the energy consumption of zymolytic production.

The invention discloses streptomyces clavuligerus and a use method of streptomyces clavuligerus in melanin production. The use method comprises the following steps of carrying out strain activation on streptomyces clavuligerus preserved by refrigeration, then carrying out propagation culture, inoculating a fermentation culture liquid with the strain liquid, carrying out fermentation culture to obtain a fermentation broth, separating a melanin product from the fermentation broth, and drying the melanin product. The strain culture liquid is inoculated with streptomyces clavuligerus and the streptomyces clavuligerus strain is subjected to propagation culture so that the strain liquid is obtained, the strain liquid is transferred to the fermentation culture liquid and is subjected to fermentation culture so that the fermentation broth is obtained, and the melanin product is separated from the fermentation broth and then is dried. The use method has simple processes, can be operated conveniently and can rich melanin production bacterium strain resources. The melanin product has stable performances and has the highest yield of 0.85g/L. The use method provides an efficient and stable method for melanin production and fills in gap of use of microbial strain in melanin production.

PCT No. PCT/ES96/00215 Sec. 371 Date Sep. 15, 1997 Sec. 102(e) Date Sep. 15, 1997 PCT Filed Nov. 15, 1996 PCT Pub. No. WO97/19187 PCT Pub. Date May 29, 1997A batch fermentation is carried out continuously or semicontinuously using strain of Streptomyces clavuligerus for the production of clavulanic acid. The fermentation process is carried out with strict control of the soluble phosphate in the medium both at the beginning and throughout the process.

The invention belongs to the biomedical field, which relates to a method for producing clavulanic acid through fermentation. Streptomyces clavuligerus are used as original strain in the method, and the method mainly comprises the step of the preparation of slant surface microbial strain, preparation of seed liquid and control of fermentation process. The invention uses cottonseed flour and starch as main nitrogen source and carbon source for fermentation media, through regulating fermentation process, the invention greatly reduces the production cost of the clavulanic acid, the fermentation potency is enhanced, and the deficiencies that the production cost is high and the fermentation potency is low in the prior art are solved.

Disruption of the two-component regulatory system encoded by the cbsA locus is associated with improved production of cephamycin C and/or clavulanic acid in Streptomyces clavuligerus.

The present invention provides a fermentative production of clavulanic acid using agro-industrial residues and solid-state fermentation technique. The process involves cultivating the selected strain of Streptomyces clavuligerus MTCC 1142 aerobically under media conditions which are not strictly acidic or alkaline and are close to neutral, with an initial moisture content of 60-75% and uses agro-industrial residues as carbon and support. The process uses complex as well as simple nitrogen sources along with small amount of mineral salt supplementation. The process is carried out at a temperature range of 25-30 DEG C for a period extending up to 120 hrs. The process is simple to operate and offers a major advantage in the fact that it requires relatively cheaper substrates for producing clavulanic acid.

The invention discloses streptomyces clavuligerus, an application, a fermentation medium and a preparation method of clavulanic acid. The streptomyces clavuligerus is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation No. of CGMCC No.19076. The invention discloses the application of the streptomyces clavuligerus to production of the clavulanic acid.According to the preparation method of the clavulanic acid disclosed by the invention, the streptomyces clavuligerus is subjected to obtain the clavulanic acid. The preparation method of clavulanic acid comprises the following steps of performing fermenting the streptomyces clavuligerus to obtain the clavulanic acid. The streptomyces clavuligerus is good in heredity stability, the yield of the clavulanic acid is high, and the titer is high.

The present invention discloses a preparation method and a compound preparation of clavulanate potassium. The preparation method comprises: filtering a Streptomyces clavuligerus fermentation sequentially with a microporous filtration membrane, a polysulfone membrane and an aromatic polyamide composite membrane; adding active carbon to the filtrate to decolorize, adding an extractant, transferring and filling into a pressure resistance container, removing gas bubbles, carrying out sealed oscillation, carrying out temperature control freezing until the water phase is frozen, taking out, and removing the water phase to obtain an extraction liquid; stirring the extraction liquid while adding a potassium carbonate solution, transferring and filling into a pressure resistance container, removing gas bubbles, carrying out sealed oscillation, carrying out temperature control freezing, taking out, removing the organic phase, slowly adding acetone in a dropwise manner after the solid melts, slowly stirring, filtering, washing with acetone, and carrying out vacuum drying to obtain clavulanate potassium; and carrying out sterile mixing on clavulanate potassium and amoxicillin according to a weight ratio of 1:5 or on clavulanate potassium and ticarcillin according to a ratio of 1:15-30. According to the present invention, the preparation method has characteristics of simple process and low cost, and the prepared product has characteristics of low impurity content and high purity.