Streptomyces clavuligerus for high-yield clavulanic acid and application of streptomyces clavuligerus

Abstract

The invention discloses a streptomyces clavuligerus for high-yield clavulanic acid, and application of the streptomyces clavuligerus. The streptomyces clavuligerus for high-yield clavulanic acid is specifically a streptomyces clavuligerus 16CF10 of which the preservation number is CGMCC No.7915 in the China General Microbiological Culture Collection Center. According to the invention, a streptomyces clavuligerus 70116 is taken as a starting strain, is subjected to metabolic engineering modification and is mutated to obtain the streptomyces clavuligerus 16CF10 CGMCC No.7915. Experimental results show that the amount of clavulanic acid produced by the strain cultivated through fermentation reaches 2.25 g / L of fermentation liquor, and is improved by about 51% compared with that the streptomyces clavuligerus 70116 serving as the starting strain, so that the streptomyces clavuligerus 16CF10 CGMCC No.7915 has a broad industrial application prospect.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of streptomyces clavulanic acid with high yield of clavulanic acid and application thereof. Background technique [0002] With the extensive use of antibiotics, the problem of drug resistance of pathogenic bacteria is becoming more and more serious. The abuse of β-lactam antibiotics such as penicillins and cephalosporins causes some pathogenic bacteria to produce β-lactamase, which degrades these antibiotics and produces drug resistance. Clavulanic acid, also known as clavulanic acid, is a secondary metabolite produced by Streptomyces clavuligerus. The antibacterial activity of clavulanic acid is very weak when acting alone, but it has a unique 3R, 5R stereo conformation, which makes it have a spectrum and irreversible β-lactamase inhibitory activity, and it is also the first to be discovered and successfully used in clinical practice β-lactamase inhibitors. In clinical ap...

AI Technical Summary

Problems solved by technology

[0003] The clavulanic acid production of wild-type Streptomyces clavulus is very low, which is far from meeting the needs of industrial fermentation. Therefore, the strains currently used in industry are often mutagenic strains obtained through multiple rounds of physical and chemical mutagenesis screening.

With the deepening of basic research on the biosynthesis of clavulanic acid, a variety of rational metabolic engineering methods have successfully increased the production of clavulanic acid in wild strains, but they still cannot surpass the mutagenic strains widely used in industry

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