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Method for Breeding New Tomato Strains Rich in Lycopene and Anti-YVCV

A technology of yellow leaf curl virus and lycopene, which is applied in biochemical equipment and methods, botanical equipment and methods, and microbial measurement/inspection, to achieve simple technology, strong TYLCV resistance, and increased lycopene content Effect

Inactive Publication Date: 2016-06-15
HANGZHOU ACAD OF AGRI SCI
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Problems solved by technology

[0007] Aiming at the lack of high-quality tomato varieties with TYLCV resistance on the market, it is difficult to aggregate the high-pigment gene dg and the disease-resistant gene Ty by using traditional techniques, and at the same time remove the linkage burden of unfavorable agronomic traits

Method used

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Embodiment

[0050] The principle and process of the invention are described in detail through the following examples.

[0051] (1) Identification method of yellow leaf curl virus (TYLCV) resistance

[0052] This program introduces a method for identification of tomato plants resistant, moderately resistant and susceptible to TYLCV.

[0053] Plants are grown in the field for natural inoculation by Bemisia tabaci. It is a preferred method to plant and inoculate local viruses in natural high-incidence fields to identify plant resistance. The disease symptoms of the plants after inoculation were observed in the field, and the disease index was counted. Symptoms of TYLCV viral disease are usually graded 0-4. Grade 0: no symptoms; Grade 1: slight yellowing of leaves; Grade 2: obvious yellowing of leaves with curling; Grade 3: growth retardation of plants, severe yellowing and curling of leaves; Grade 4: severe growth retardation of plants with Yellowish, curled leaflets.

[0054] When the ...

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Abstract

The invention discloses a method for breeding new tomato strains rich in lycopene and resistant to yellow leaf curl virus. Screening of recessive mutant gene dg-specific Caps molecular markers based on tomato genome DNA sequence characteristics. A pair of Caps molecular markers closely linked to the anti-yellow leaf curl virus (TYLCV) gene Ty-3 was screened; the tomato material containing the TYLCV resistance gene and the tomato mutant dg material were crossed with common tomato elite inbred lines, and Through multiple generations of selfing, single plants were screened on the basis of dg and Ty-3 molecular markers, and an excellent inbred line with dg and Ty-3 genes was obtained. This method is also applicable to all hybrids obtained by crossing the inbred line with other tomato inbred lines.

Description

technical field [0001] The invention belongs to the field of agricultural breeding, in particular to a tomato breeding method for simultaneous transfection of high lycopene gene and anti-yellow leaf curl virus gene by using molecular markers. Background technique [0002] Tomato is one of the most important crops with high economic value in the world, and it is one of the vegetable crops with the highest annual yield in the world. Many cultivars with excellent traits have been bred using traditional breeding methods, such as resistance to various diseases, stress resistance, storage and transportation, and nutrient-enhanced tomato varieties. In modern society, a trait of great interest is the development of secondary metabolites that can confer health benefits in humans. Tomatoes contain a variety of carotene with antioxidant effects, which have a strong health care effect on the human body. Epidemiological studies have shown that lycopene can reduce the efficacy of some c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 郑积荣刘松瑜王慧俐秦力王燕
Owner HANGZHOU ACAD OF AGRI SCI
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