Antibacterial peptide fermentation culture medium formula

A fermentation medium and antibacterial peptide technology are applied in the field of antibacterial peptide fermentation medium prescription, and can solve the problems of long fermentation period, high cost, easy generation of bubbles in the medium and the like

Inactive Publication Date: 2014-12-31
LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a prescription for antimicrobial peptide fermentation medium, which solves the problems of easy generation of air bubbles in the fermentation process of the existing medium, high cost, difficult sterilization and long fermentation period

Method used

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  • Antibacterial peptide fermentation culture medium formula

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Embodiment 1

[0009] Inoculate a Bacillus subtilis on the culture medium, the medium formula is: 2% corn steep liquor dry powder, 2% yeast powder, 3% peptone, 1%-1.5% magnesium sulfate, 1%-1.5% potassium dihydrogen phosphate, The rest is soft water. Industrially, the process conditions for inoculating the strains on the medium of the formula of the present invention for fermentation are: fermentation temperature 34-35°C, aeration ratio 1:1, tank pressure 0.01-0.03MPa, stirring speed 150 rpm, pH6- 6.5. The fermentation period is 16 hours, and the fermentation liquid with the antimicrobial peptide content of 2490 μg / ml-2510 μg / ml can be obtained.

Embodiment 2

[0011] A kind of Bacillus subtilis was inoculated on the culture medium, and the formula of the culture medium was: 2% corn steep liquor dry powder, 2% yeast powder, 3% peptone, 0.75% magnesium sulfate, 1% potassium dihydrogen phosphate, and the rest was soft water. The fermentation process conditions are: temperature 34°C, ventilation ratio 1:1, tank pressure 0.03MPa, stirring rate 150 rpm, pH 6, fermentation cycle 16.4 hours, and a fermentation broth with an antimicrobial peptide content of 2505 μg / ml.

Embodiment 3

[0012] Embodiment 3: A kind of Bacillus subtilis is inoculated on the medium, and the medium formula is: corn steep liquor dry powder 2%, yeast powder 2%, peptone 3%, magnesium sulfate 1%, potassium dihydrogen phosphate 0.75%, all the other are soft water. The fermentation process conditions are: temperature 35°C, ventilation ratio 1:1, tank pressure 0.01MPa, stirring speed 150 rpm, pH 6.5, fermentation cycle 16.1 hours, and a fermentation broth with an antimicrobial peptide content of 2501 μg / ml.

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Abstract

The invention discloses an antibacterial peptide fermentation culture medium formula which comprises the following components in percentage by mass: 2% of dry corn steep liquor powder, 2% of yeast powder, 3% of peptone, 1-1.5% of magnesium sulfate, 1-1.5% of monopotassium phosphate and the balance of soft water. The raw materials used in the fermentation production formula are all water-soluble, sterilization is convenient to perform, foam generation is prevented, the fermentation process is easy to control, the separation of antibacterial peptide is facilitated, the antibacterial peptide content is high, the cost is low, and the fermentation period is short.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a prescription of an antimicrobial peptide fermentation medium. Background technique [0002] Antimicrobial peptides are small molecular peptides with biological activity induced by organisms. Most of these active peptides have the characteristics of alkalinity, thermal stability and broad-spectrum antibacterial properties. Compared with antibiotics, it has no harm to normal cells of humans and animals, has no side effects, and has no residues. It has broad application prospects in the fields of antibacterial medicine, food preservation and fresh-keeping, and feed additives. [0003] However, at present, there are many problems in the industrial production process of antibacterial peptide fermentation medium, such as high cost, difficult separation of antimicrobial peptide and medium components, bubbles generated in the fermentation process make the fermentation process d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12R1/125
Inventor 胡国春郭文江赵福生王庆芳郭玉萍
Owner LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
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