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Construction method for mouse type 2 diabetes mellitus (T2DM) animal experiment model

A type 2 diabetes and animal experiment technology, which is applied in the field of building animal experimental models of type 2 diabetes in mice, can solve the problems of inconsistent reports and achieve the effects of light weight, saving experimental costs, and small doses

Active Publication Date: 2015-01-07
QINGHAI NORMAL UNIV
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Problems solved by technology

Feeding high-fat and high-sugar feed combined with low-dose streptozotocin induction is a commonly used model-making method, but the strain and age of experimental mice, feeding time of high-fat and high-sugar , diabetic mice modeling standards, etc., related literature reports are different

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  • Construction method for mouse type 2 diabetes mellitus (T2DM) animal experiment model

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Embodiment Construction

[0007] The preferred technical scheme of the method for constructing an animal experimental model of type 2 diabetes in the patent of the present invention will be further described below with reference to the accompanying drawings and examples.

[0008] Main technical scheme: raw materials and auxiliary materials: male Kunming mice, distilled water, streptozotocin, cholesterol, sodium cholate, etc. Main equipment: blood glucose meter, blood glucose test paper, blood lipid meter, cholesterol test paper, triglyceride test paper, high density lipoprotein test paper, disposable syringe, etc.

[0009] The embodiment of the construction method of a type 2 diabetes animal experimental model of the patent of the present invention is as follows: take 10-20-day-old male Kunming mice, preferably inject 65 mg / Kg streptozotocin intraperitoneally at one time, and give it from the time of free food. After continuous feeding of high-fat and high-sugar feed for 5 weeks, an automatic blood glu...

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Abstract

The invention patent relates to the technical field of animal experiment model construction, in particular to a construction method for a mouse type 2 diabetes mellitus (T2DM) animal experiment model. The method includes the following steps: male Kunmin mice at the age of 7 to 31 days are selected; 50 to 75 mg / Kg (milligrams per kilogram) of streptozotocin is taken by the mice through intraperitoneal injection at a time; after the mice are continuously fed with high-fat high-glucose feed for 4 to 6 weeks upon free eating, the mice of which fasting blood-glucose is above 7.0 millimoles per liter, blood glucose in a 2-hour oral glucose tolerance test is above 11.1 millimoles per liter, cholesterol is above 5.7 millimoles per liter, triglyceride is above 1.7 millimoles per liter, and high-density lipoprotein is below 1.0 millimole per liter at the same time are defined as T2DM animal experiment models. The model can simulate the T2DM characteristics of humans, and adopts multiple testing indicators to accurately define T2DM; due to the light weights of the underage animals, the streptozotocin dosage is small, so that the experiment cost is reduced.

Description

[0001] technical field [0002] The invention relates to the technical field of animal experimental model construction, in particular to a construction method of a mouse type 2 diabetes animal experimental model. Background technique [0003] Diabetes has seriously threatened human life and health, and is the most serious chronic non-communicable disease after tumor and cardiovascular disease. With the development of my country's social economy, the improvement of people's living standards, and the change of diet structure, the incidence of diabetes in my country is as high as 9.7%. A survey in 2010 showed that there are currently more than 92.4 million diabetic patients in China, which has become the world's largest country with diabetes. Among many diabetic patients, more than 90% have type 2 diabetes. The establishment of a stable, reliable and efficient animal model is a prerequisite for the study of the pathogenesis of type 2 diabetes and therapeutic interventions. H...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7008A01K67/02
CPCA01K67/02A61K31/7008
Inventor 曾阳唐勋尚军乔枫马秀梅宋永朋张倩茹余涛孙玉侠王永萍
Owner QINGHAI NORMAL UNIV
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