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A capillary-based flow type polymerase chain reaction circulation heater and heating method

A cyclic heating and chain reaction technology, applied in chemical instruments and methods, bioreactors/fermenters for specific purposes, heating or cooling equipment, etc., can solve the problem of uneven heating of samples, prone to leakage, and low repeatability and other problems, to achieve the effect of easy and uniform temperature distribution, improved heat transfer rate, and good repeatability

Active Publication Date: 2016-08-17
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of microchips has the characteristics of handicrafts, that is, strong individuality and low repeatability; secondly, the chips are made by bonding, which is prone to leakage; the channel of the microchip is rectangular, and the capillary of the existing detection equipment is circular. At the interface between the pipe and the chip, the droplet is in frequent contact with the wall of the channel, and droplet fusion and sample contamination often occur; finally, this method is heating on one side and cooling on the other side, so the sample is heated unevenly

Method used

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  • A capillary-based flow type polymerase chain reaction circulation heater and heating method
  • A capillary-based flow type polymerase chain reaction circulation heater and heating method
  • A capillary-based flow type polymerase chain reaction circulation heater and heating method

Examples

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Effect test

Embodiment 1

[0023] A capillary-based flow polymerase chain reaction spiral heater of the present invention:

[0024] The whole device is composed of a bracket 1, a heating plate 2, a constant temperature channel 3, a capillary 5 and temperature controllers 6-1, 6-2, and 6-3. The bracket 1 is a triangle, and the three corners of the triangle are rounded. The triangle bracket 1 There is a channel 1-1 on each side; the constant temperature channel is composed of two identical modules 3-1 that are relatively fastened together. The module 3-1 is in the shape of a rectangular parallelepiped. A row of semicircular capillary channels 3-3, the capillary 5 is a flexible pipe, and the temperature controller consists of three temperature controllers 6-1, 6-2, 6-3 to independently control the temperature of each heating plate;

[0025] Install the heating plate 2 and the module 3-1 in sequence in each channel 1-1 on the side of the support 1; the module 3-1 has the capillary channel 3-3 facing outward...

Embodiment 2

[0030] The triangular bracket is made of plexiglass, with a thickness of 20mm, a side groove width of 80mm, and a depth of 8mm. The heating plate adopts an aluminum plate heater, and the power supply is a 24V DC power supply with a power of 20W. The single module of the constant temperature channel is an aluminum block with a thickness of 4mm, a length of 80mm, and a width of 20mm. The diameter of the semicircular channel is 0.8mm and the spacing is 0.2mm. The capillary adopts a silicone tube with an outer diameter of 0.8mm. Temperature controller Choose REX-C700 temperature controller. Three heating groups, two groups are used for heating; one group is used for cooling, which constitutes two-temperature PCR. All components are integrated on a flat panel and finally sealed in a case.

Embodiment 3

[0032] The triangular bracket is made of plastic, with a thickness of 20mm, a side groove width of 80mm, and a depth of 8mm. The heating plate adopts a stainless steel electric heater, and the power supply is a 24V DC power supply with a power of 20W. The single module of the constant temperature channel is made of stainless steel, with a thickness of 4mm, a length of 80mm, and a width of 20mm. The diameter of the semicircular channel is 0.8mm, and the spacing is 0.2mm. The capillary adopts a silicone tube with an outer diameter of 0.8mm. Temperature controller Choose REX-C700 temperature controller. The temperature of each group of constant temperature baths is controlled and displayed by an independent PID controller; three groups of heating groups, two of which are used for heating, respectively controlling 65 and 95 degrees; the remaining group is used for cooling (using fans), which constitutes a two-temperature PCR. All components are integrated on a flat panel and fin...

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Abstract

The invention discloses a flow polymerase chain reaction (PCR) circulating heating apparatus based on capillaries and a heating method. According to the method, PCR of samples in the capillaries is achieved by adopting a method that the capillaries penetrate through three different constant-temperature areas circularly, and a new process that amplification is achieved in a three-dimensional flow process of the samples under the condition of static temperature control can be formed. In a temperature cycling process, a fluid in each capillary carries sample droplets to flow into one end of a heating device, then penetrates among the three temperature areas along the capillary, and flows out from the heating device after completing a predetermined number of heating cycles to enter a detection device. Compared with a planar micro-chip method, the heating method disclosed by the invention is easily combined with existing biological detection equipment, and meanwhile, because the capillaries with standard batch production are adopted, the capillaries are higher in repeatability of working performance, are very suitable for being used as plugins, and can perform combined operation with the existing high-precision biological detection equipment.

Description

technical field [0001] The invention belongs to the technical field of microfluidic temperature control, and in particular relates to a capillary-based flow polymerase chain reaction (PCR) circulation heater and a method for performing circulation heating in the instrument. Background technique [0002] Polymerase chain reaction (PCR) uses DNA denaturation at a high temperature of 95°C in vitro to become a single strand. At low temperature (usually around 60°C), primers and single strands are combined according to the principle of complementary base pairing, and then the temperature is adjusted. To the optimum reaction temperature of DNA polymerase (about 72°C), DNA polymerase synthesizes complementary chain along the direction from phosphoric acid to five-carbon sugar (5'-3'). The PCR instrument based on polymerase is actually a temperature control device, which can well control the denaturation temperature, renaturation temperature and extension temperature. The tradition...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/34
CPCB01L7/525B01L7/54B01L2300/0838
Inventor 何立群焦冬生罗昭锋
Owner UNIV OF SCI & TECH OF CHINA
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