Biocontrol Trichoderma F18, and preparation method and application thereof

A technology for bio-control of Trichoderma and Trichoderma viride, applied in biochemical equipment and methods, microorganism-based methods, botanical equipment and methods, etc., can solve problems such as single function

Active Publication Date: 2015-01-07
山东济清科技服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Trichoderma strains obtained above have only been improved in terms of biocontrol or drug resistance, and their functions are relatively single.

Method used

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  • Biocontrol Trichoderma F18, and preparation method and application thereof
  • Biocontrol Trichoderma F18, and preparation method and application thereof
  • Biocontrol Trichoderma F18, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 A kind of biocontrol Trichoderma F18 and preparation method thereof

[0050] A biocontrol Trichoderma F18 that efficiently degrades carbendazim, its biological strain preservation number is: CGMCC No.9486, the preservation date is: August 5, 2014, and the preservation unit is: China Microorganism Culture Collection Management Committee General Microbiology Center.

[0051] The method for preparing said Trichoderma F18, the specific steps are as follows:

[0052] (1) Mycelia culture: Trichoderma harzianum Tr1 (Trichoderma Tr1 protoplasts such as figure 1 shown) and Trichoderma viride LTR-2 were inoculated on PDA plates, cultured at 28°C for 3-5 days, prepared conidia suspensions of Trichoderma harzianum Tr1 and Trichoderma viride LTR-2 with sterile water, and finally suspended Liquid concentration is 1×10 6 cells / mL, transfer the spore suspension to the PD liquid medium according to the inoculum amount accounting for 1% of the volume of the PD liquid med...

Embodiment 2

[0090] Embodiment 2. Trichoderma fusion child F18 inoculant agent is to the control pot experiment of wheat sheath blight

[0091] 1) Seeds and their treatment

[0092] The wheat variety was Zhongyou-16, which was purchased from Shandong Academy of Agricultural Sciences. First, use 70% alcohol for disinfection, then wash with sterile water, and finally place them in a 30°C incubator for germination.

[0093] 2) Potted soil and its treatment

[0094] Take the soil from the Institute of Biology, Shandong Academy of Sciences, sieve to remove stones, weeds and other sundries, add the purchased peat soil at a volume ratio of 1:1, mix the soil samples, sterilize at 121°C, 120min for 40min, and naturally Air dry and set aside.

[0095] 3) Pot method

[0096] Use a balance to weigh 1000 grams of sterilized soil samples, pour them into flower pots, put 8 grains of millet grains covered with pathogenic bacteria in each pot (placed in the same position in each cup), and use a balance...

Embodiment 3

[0113] Example 3. Research on the remediation of carbendazim pesticide residue-contaminated soil by simulated Trichoderma F18 bacterial agent under laboratory conditions

[0114] 1) Degradation effect of Trichoderma F18 wettable powder on carbendazim in sterilized soil

[0115] Put 5g of carbendazim and 5g of Trichoderma F18 wettable powder into 150ml of sterile water to make a suspension, then mix it evenly with 1000g of sterilized soil passed through a 10-mesh sieve, adjust the water content to 15%, and then put it into the sterilized fungus in a pot. Wrap the mouth of the basin with gauze, and culture it at 25°C with moisture, and take 10g soil samples every 7 days to measure the content of carbendazim. At the same time, 5g of carbendazim, 5g of solid medium (not inoculated with Trichoderma F18 wettable powder) and 1000g of sterilized soil passed through a 10-mesh sieve were mixed as a control. Each treatment was repeated 3 times. All the above operations need to be perf...

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Abstract

The invention relates to a biocontrol Trichoderma F18, and a preparation method and application thereof. The biological culture collection number of the biocontrol Trichoderma F18 is CGMCC No.9486, the collection date is August 5th, 2014, and the collection unit is China General Microbiological Culture Collection Center. The preparation method comprises the following steps: mycelium culture, protoplast preparation, protoplast fusion and fusant screening. The biocontrol Trichoderma F18 has the functions of carbendazol degradation and bacteriostasis.

Description

technical field [0001] The invention relates to a biocontrol Trichoderma F18 and its preparation method and application, in particular to a biocontrol Trichoderma F18 with carbendazim degradation and antibacterial effect obtained through protoplast fusion technology, and belongs to the field of biotechnology. Background technique [0002] Carbendazim is a high-efficiency broad-spectrum benzimidazole fungicide, widely used in field, economic crops, vegetables and fruit disease control, and can also be used for mildew prevention in paper, rubber, textile, leather and other industries , and is also the active component of other fungicides such as benomyl and thiophanate-methyl. my country is a big country in the production and use of carbendazim. In 2011, the production of carbendazim exceeded 40,000 tons for the first time, accounting for 1 / 4 of the total fungicides in my country. While carbendazim has been widely used in agricultural production in my country, the problem of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/15C12N15/04A62D3/02A01N63/04A01P3/00C12R1/885A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28A01N63/30C12N15/04C12N1/145C12R2001/885
Inventor 黄玉杰张新建王加宁陈贯虹孔学王磊磊郑立稳张闻杨合同
Owner 山东济清科技服务有限公司
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