Administration method and administration device for experimental animal brain

Abstract

The invention discloses an administration method for an experimental animal brain; by adopting a method for burying a catheter into the experimental animal brain, injection to the experimental animal brain is more convenient. According to the administration method, a special successive administration device is adopted, and a mouse or a rat is taken as an experimental animal, and the administration method comprises the following steps: 1, narcotizing the experimental animal with 30 percent of chloral hydrate, fixing the experimental animal on a mouse brain locator, and determining the position of a paracele; 2, burying an administration catheter into the paracele of the experimental animal according to the step, and fixing the catheter to the head of the experimental animal by dental cement when cerebrospinal fluid overflows from the catheter; 3, in 4 days after the catheter is buried successfully, performing successive intramuscular injection every day with 0.2ml of 0.8 million units of penicillin sodium, and diminishing inflammation of the experimental animal; 4, performing administration: sucking up a right amount of to-be-experimented drug solution by a 10mul microinjector, and partially inserting the syringe needle of a microsyringe into the catheter buried into the experimental animal brain, wherein the insertion depth is 0.5-2.0cm, and successive administration can be performed on the mice brain from the catheter.

Description

technical field [0001] The invention belongs to the technical field of medicine, relates to a method for administering medicine to mice and rats, and relates to a method and a device for administering medicine to the brain of experimental animals. [0002] and drug delivery devices. Background technique [0003] In the process of modern drug research and development, drug activity screening is one of the important links. The existing drug activity screening mostly adopts in vitro screening method and in vivo screening method. Among them, the in vivo screening method can more directly reflect the effect of the drug in the in vivo environment, and this method plays an important role in the drug screening process. [0004] The use of in vivo drug screening methods must first establish a suitable animal model, and secondly requires a suitable drug delivery method. Drug researchers have established many effective animal models in various ways, such as hypertension models, senil...

AI Technical Summary

Problems solved by technology

However, many problems are also encountered in the application of the intracerebroventricular injection method. For example, in the process of some drug screening, multiple administrations are required. Due to the fact that physical damage is easily caused during the administration of the brain, the experimental animals are caused by multiple injections. If the damage increases, it may affect the experimental results, and in severe cases, it will cause the death of the experimental animals.

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