A kind of Torula delbrueckii and application thereof

Abstract

The invention discloses Torula delbrueckii and application thereof, and its preservation numbers are CGMCC No.8740 and CGMCC No.8742. The present invention adopts ultraviolet mutagenesis method to carry out mutagenesis to saccharomycete, then screens the mutant strain with high activity phytase under acidic condition, makes the mutant phytase that mutant strain produces can be better under the pH condition of gastric environment. Hydrolyzed phytic acid. The mutant strain can be used to produce feed or edible phytase, and can also be used to produce microbial phosphate fertilizer or biological organic phosphate fertilizer. In addition, the mutated phytase gene sequence can be cloned into prokaryotic or eukaryotic gene expression vectors, and genetically engineered strains or transgenic plants can be constructed by genetic engineering techniques to efficiently express the mutated phytase gene to produce mutated phytase .

Description

technical field [0001] The invention relates to yeast mutant strains and applications thereof, in particular to Torula delbrueckii producing phytase with high catalytic activity and applications thereof. Background technique [0002] Phytic acid, also known as phytic acid, contains 6 phosphate groups and is an important storage form of phosphorus in feed. Phosphorus is an essential mineral element for animals, but monogastric animals lack phytase (Phytase, an enzyme that catalyzes the hydrolysis of phytic acid and phytate) in the body of monogastric animals, resulting in the utilization rate of phosphorus in feed being only 1 / 3 or lower. In order to supplement the deficiency of available phosphorus, inorganic phosphate must be added to the feed, commonly used as calcium hydrogen phosphate and bone meal. This not only greatly increases the cost of feed, but also a large amount of phytate phosphorus cannot be utilized and is directly excreted from the body, resulting in wast...

AI Technical Summary

Problems solved by technology

Solved the technical problem of low activity of phytase under acidic conditions in the prior art

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