Function and application of blood shear stress responding protein 1 in treatment of atherosclerosis

An atherosclerosis and drug technology, applied in the field of gene function and application, can solve the problems of unexplained biological function and possible mechanism of action, and achieve the effect of promoting atherosclerosis

Active Publication Date: 2015-02-04
武汉惠康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above research basis, we believe that RECS1 may play an important role in the characteristic pathophysiological process of atherosclerosis, but its biological function and possible role in the characteristic pathophysiological process of atherosclerosis mechanism has not been elucidated

Method used

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  • Function and application of blood shear stress responding protein 1 in treatment of atherosclerosis
  • Function and application of blood shear stress responding protein 1 in treatment of atherosclerosis
  • Function and application of blood shear stress responding protein 1 in treatment of atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Mouse Atherosclerosis Model (AS) Obtained

[0045] 1. Grouping of experimental animals: 8 weeks old, weighing 19-25g, male, ApoE - / - mice and RECS1 - / - ApoE - / - Mice were fed high-fat diet (Western Diets, HFD) and low-fat diet (Normal chow, NC) respectively, ApoE - / - HFD group, ApoE - / - NC group, RECS1 - / - ApoE - / - HFD group, RECS1 - / - ApoE - / - The NC group consisted of 4 groups with 20 rats in each group.

[0046] 2. Atherosclerosis model induced by high-fat diet:

[0047] Using ApoE - / - mice and RECS1 - / - ApoE - / - In mice, AS models were established, and phenotype correlation analysis was performed to clarify the role of RECS1 gene in atherosclerotic diseases. From the age of 8 weeks, the mice in the HFD group were sacrificed after 28 weeks of high-fat diet, and the samples were collected in the NC group after 28 weeks of low-fat diet.

Embodiment 2

[0048] Example 2 Determination of plaque area in AS model mice

[0049] 1. Final mouse tissue harvesting

[0050] Mice were fed with high-fat or low-fat diet until 28 weeks, weighed, anesthetized with 3% pentobarbital sodium, 90 mg / kg, fixed on the sampling board with a needle, moistened the skin of the chest and abdomen of the mouse with gauze, and used Ophthalmic scissors cut open the chest cavity, expose the heart, cut open the right atrial appendage, insert the needle of the infusion set into the left ventricle, inject 10-15mL PBS buffer slowly with a 50mL syringe, wait until the right atrial appendage effluent is clear, replace it with 4% Polymer Formaldehyde continued to inject 10-15mL. After the perfusion, the thoracoabdominal viscera were removed and only the heart was kept. Put the mouse under a microscope, separate the fascia and fatty tissue around the aortic arch, cut the heart at the beginning of the ascending aorta, cut at the middle of the thoracic aorta, and ...

Embodiment 3

[0063] Example 3 Determination of Plaque Stability in AS Model Mice

[0064] 1. Area Size Determination of the Center of Aortic Sinus Necrosis

[0065] For hematoxylin and eosin staining (HE staining), the method is the same as in Example 2.4, and a tissue containing cholesterol crystals and no nucleus fiber structure is selected to take pictures under a microscope.

[0066] Determination of the area of ​​the necrosis center: use Image-Pro Plus 6.0 image analysis software to circle the area of ​​the necrosis center.

[0067] 2. Determination of collagen content in aortic sinus:

[0068] Sirius red (PSR) staining, the main steps are: take aortic sinus paraffin white slices and bake at 55°C for 30 minutes → xylene for 2 minutes, 3 times → 100% alcohol for 1 minute → 95% alcohol for 1 minute → 70% alcohol for 1 minute → rinse with running water for 10 minutes → Double distilled water for 1min→mass fraction 0.2% phosphomolybdic acid for 2min→0.1% Sirius red picric acid solution ...

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Abstract

The invention discloses a function and an application of a blood shear stress responding protein 1 (RECS1) in treatment of atherosclerosis and belongs to the field of functions and applications of genes. In the invention, an ApoE -/- mouse and an RECS1 -/- ApoE -/- mouse are employed as experimental subjects and an atherosclerosis model is obtained through high-fat diet induction. A result proves that compared with the ApoE -/- mouse, a RECS1 gene defect can significantly reduce a plague area in aorta, enhance stability of aortic sinus plagues and significantly alleviate inflammatory response, which indicate that the RECS1 in the atherosclerosis mainly has effects of promoting formation of a plague in the aorta, especially promoting the atherosclerosis. On the basis of the functions of the RECS1, the RECS1, as a drug target, can be used for screening a drug for preventing, alleviating and/or treating the atherosclerosis. An inhibitor of the RECS1 can be used for preparing a drug for preventing, alleviating and/or treating the atherosclerosis.

Description

[0001] technical field [0002] The invention belongs to the field of gene function and application, and specifically relates to the function and application of a blood shear stress response protein 1 (RECS1) in the treatment of atherosclerosis, specifically RECS1 in the preparation of prevention, alleviation and / or treatment of atherosclerosis Drug application in sclerosis. Background technique [0003] Cardiovascular and cerebrovascular diseases are the main cause of death in many developed countries, and the morbidity and mortality in my country are also increasing year by year. The basis of cardiovascular and cerebrovascular diseases is atherosclerosis (Atheosclersis, AS). Atherosclerosis can thicken and harden the arterial wall and narrow the lumen, leading to many cardiovascular and cerebrovascular events. Acute stenosis and occlusion of coronary arteries caused by rupture of atherosclerotic unstable plaques, platelet aggregation, and thrombus formation are important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K48/00A61K39/395A61K45/00A61P9/10
CPCY02A50/30
Inventor 李红良赵光年王丕晓邓克穷
Owner 武汉惠康基因科技有限公司
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