Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of production method of Bdellovibrio microecological preparation

A micro-ecological preparation, Bdellovibrio phage technology, applied in the field of breeding, can solve the problems of reduced cracking activity and short shelf life of Bdellovibrio phage, and achieve the effect of reducing dosage, reducing production cost and extending shelf life

Active Publication Date: 2016-06-29
广州利洋水产科技股份有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of Bdellovibrio phage probiotics and production method thereof, adopt the slow-release technology of host bacterium, solve the problems such as the short shelf life of Bdellovibrio phage in the industrial production process, cracking activity reduction, etc., Improve the effect of bacteriophage Bdellovibrio preparation on aquatic animal disease control and water quality improvement

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of production method of Bdellovibrio microecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: host bacterium is fixed with polyvinyl alcohol, and specific process is as follows:

[0028] (1) Preparation of host bacteria suspension

[0029] The cultured Escherichia coli was sterilized under high pressure at 115°C for 30 minutes, then centrifuged at 10,000 rpm in a tubular high-speed centrifuge, and the bacteria were collected and diluted to a concentration of 10 with phosphate buffer. 12 cfu / mL host bacterial suspension, stored at 4°C for later use;

[0030] (2) Preparation of sustained-release granules

[0031] Dilute the host bacterial suspension by 2 times with water, then add 10% polyvinyl alcohol, fully mix and dissolve, add 1 volume of vegetable oil and stir thoroughly to form a sphere, set and solidify at -20°C for 8 hours, and wash for later use. (3) Preparation of culture medium

[0032] Dilute the pH7.4 phosphate buffer 100 times with water, then add 40mg / L CaCl 2 and 40mg / L of MgCl 2 .

[0033] (4) Preparation of seeds

[0034] Add...

Embodiment 2

[0037] Embodiment 2: The host bacterium is fixed with sodium alginate, and the specific process is as follows:

[0038] (1) Preparation of host bacteria suspension

[0039] The cultured Escherichia coli was sterilized under high pressure at 115°C for 30 minutes, then centrifuged at 10,000 rpm in a tubular high-speed centrifuge, and the bacteria were collected and diluted to a concentration of 10 with phosphate buffer. 12 cfu / mL host bacterial suspension, stored at 4°C for later use;

[0040] (2) Preparation of sustained-release granules

[0041] Dilute the host bacterial suspension by 1 time with water, add 2% sodium alginate, mix well to dissolve, and then drop in 3% CaCl 2 , cross-link for 30 minutes, and wash for later use.

[0042] (3) Preparation of culture medium

[0043] Dilute the pH7.2 phosphate buffer 20 times with water, then add 20mg / L CaCl 2 and 20mg / L of MgCl 2 .

[0044] (4) Preparation of seeds

[0045] Add 5% of the host bacteria suspension prepared in...

Embodiment 3

[0048] Embodiment 3: use gelatin and sodium alginate to fix the host bacteria, the specific process is as follows:

[0049] (1) Preparation of host bacteria suspension

[0050] The cultured Escherichia coli was sterilized under high pressure at 115°C for 30 minutes, then centrifuged at 10,000 rpm in a tubular high-speed centrifuge, and the bacteria were collected and diluted to a concentration of 10 with phosphate buffer. 12 cfu / mL host bacterial suspension, stored at 4°C for later use;

[0051] (2) Preparation of sustained-release granules

[0052] Dilute the host bacterial suspension by 1 time with water, add 7% gelatin and 3% sodium alginate, mix well to dissolve, and then add 4% CaCl dropwise 2 , cross-link for 60 minutes, and wash for later use.

[0053](3) Preparation of culture medium

[0054] Dilute the pH7.2 phosphate buffer 10 times with water, then add 60mg / L CaCl 2 and 60mg / L of MgCl 2 .

[0055] (4) Preparation of seeds

[0056] Add 15% of the host bacteri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of cultivation, and discloses a production method of a phage bdellovibrio microecological preparation. The production method comprises the following steps of (1) preparing host bacteria suspension; (2) preparing slow release particles; (3) preparing cultivation liquid; (4) preparing seeds; (5) preparing the bdellovibrio preparation. By the slow release technology of host bacteria, the defects that an existing phage bdellovibrio preparation is short in quality guarantee period and unstable in bacteria content are overcome, and the effects of preventing and treating aquatic livestock diseases and improving the water quality of the phage bdellovibrio preparation are enhanced.

Description

technical field [0001] The invention belongs to the technical field of cultivation, and in particular relates to a production method of a Bdellovibrio microecological preparation. Background technique [0002] Bdellovibrio phage is a type of parasitic bacteria that specializes in preying on bacteria. It can lyse Vibrio, Pseudomonas, Aeromonas, etc. in water in a short period of time, reducing the number of pathogenic bacteria. It is limited to a low level, and at the same time, it can effectively control the COD, sulfide and ammonia nitrogen content in the aquaculture water, and can improve the intestinal absorption and immune level of the aquaculture body. The Bdellovibrio living bacteria preparation can be used not only as a feed additive, but also as a water quality improver for directly splashing aquaculture water. Therefore, using it as a natural biological purification factor and "active antibiotic" in the aquaculture water environment has broad development prospects ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 江文涛马加军储玉龙
Owner 广州利洋水产科技股份有限公司