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Hepatitis c virus antigen-antibody joint detection kit

A hepatitis C virus and kit technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as increased workload and increased cost, and achieve the goal of reducing the missed detection rate, avoiding missed detection, and ensuring stability. Effect

Active Publication Date: 2015-03-11
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of missed detection of hepatitis C in the current clinical detection, and the increase of workload and cost caused by the operation of various kits, the present invention provides a kit for combined detection of hepatitis C virus antigen-antibody. Kit for sensitive and accurate detection of hepatitis C virus

Method used

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  • Hepatitis c virus antigen-antibody joint detection kit
  • Hepatitis c virus antigen-antibody joint detection kit
  • Hepatitis c virus antigen-antibody joint detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A hepatitis C virus antigen-antibody combined detection kit comprises control serum, sample diluent, concentrated washing solution, chromogenic reagent, stop solution, protein-coated plate and enzyme conjugate.

[0038]The control serum used in the kit includes positive control serum and negative control serum. The main components of the positive control serum are HCV-cAg strong positive serum and subtype antibodies; the negative control serum is the normal human serum without HCV-cAg as the main component. The main component of sample diluent is the PBS that adds goat serum; The main component of concentrated washing liquid is NaCl, phosphate, Tween-20; The component is TMB.2HCL; the main component of the stop solution is 2M H 2 SO 4 .

[0039] Protein-coated plates are prepared by the following steps:

[0040] Dilute the antigen and antibody to a certain concentration with 0.02 mol / L phosphate buffer solution at pH 7.4; add the diluted coating solution at 100 μL / we...

Embodiment 2

[0055] A hepatitis C virus antigen-antibody combined detection kit comprises control serum, sample diluent, concentrated washing solution, chromogenic reagent, stop solution, protein-coated plate and enzyme conjugate.

[0056] The control serum used in the kit includes positive control serum and negative control serum. The main components of the positive control serum are HCV-cAg strong positive serum and subtype antibodies; the negative control serum is the normal human serum without HCV-cAg as the main component. The main component of sample diluent is the PBS that adds goat serum; The main component of concentrated washing liquid is NaCl, phosphate, Tween-20; The component is TMB.2HCL; the main component of the stop solution is 2M H 2 SO 4 .

[0057] Protein-coated plates are prepared by the following steps:

[0058] Dilute the antigen and antibody to a certain concentration with 0.02 mol / L phosphate buffer solution at pH 7.4; add the diluted coating solution at 100 μL / w...

Embodiment 3

[0065] A hepatitis C virus antigen-antibody combined detection kit comprises control serum, sample diluent, concentrated washing solution, chromogenic reagent, stop solution, protein-coated plate and enzyme conjugate.

[0066] The control serum used in the kit includes positive control serum and negative control serum. The main components of the positive control serum are HCV-cAg strong positive serum and subtype antibodies; the negative control serum is the normal human serum without HCV-cAg as the main component. The main component of sample diluent is the PBS that adds goat serum; The main component of concentrated washing liquid is NaCl, phosphate, Tween-20; The component is TMB.2HCL; the main component of the stop solution is 2M H 2 SO 4 .

[0067] Protein-coated plates are prepared by the following steps:

[0068] Dilute the antigen and antibody to a certain concentration with 0.02 mol / L phosphate buffer solution at pH 7.4; add the diluted coating solution at 100 μL / w...

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Abstract

The invention belongs to the technical field of detection of hepatitis c, and particularly relates to a hepatitis c virus antigen-antibody joint detection kit. The kit comprises a protein coated plate, enzyme conjugates, control serum, sample diluent, washing concentrate, a color developing agent and a stop solution. According to the kit, anti-hepatitis c virus core antigen antibodies and other hepatitis c virus recombinant protein subunits are fixed on a solid-phase vector, so that hepatitis c virus core antigens and multiple subtype antibodies in a sample can be adsorbed onto the solid-phase vector through immune reaction, and missed detection is avoided; the enzyme conjugates in the kit are used for the enzyme labeling of corresponding antibodies and antigens paired with coated antibodies and antigens, and can be paired with the coated antibodies and antigens for use, so that the detection specificity is ensured; a protein structure-free macromolecular substance is adopted as a blocking solution, and meanwhile, a protein protectant is added into the blocking solution, so that the stability of the kit is effectively ensured.

Description

technical field [0001] The invention belongs to the technical field of hepatitis C detection, in particular to a hepatitis C virus antigen-antibody combined detection kit. Background technique [0002] Viral hepatitis C, referred to as hepatitis C, hepatitis C, is a viral hepatitis caused by hepatitis C virus (HCV) infection, mainly through blood transfusion, acupuncture, drug abuse, etc. According to the statistics of the World Health Organization, the global The infection rate of HCV is about 3%, it is estimated that about 180 million people are infected with HCV, and there are about 35,000 new cases of hepatitis C every year. Hepatitis C is a global epidemic, which can lead to chronic inflammation, necrosis and fibrosis of the liver, and some patients can develop liver cirrhosis and even hepatocellular carcinoma (HCC). In the next 20 years, the mortality rate related to HCV infection (liver failure and death caused by hepatocellular carcinoma) will continue to increase, ...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/531G01N33/543
CPCG01N33/54333G01N33/56983G01N33/5767
Inventor 谢清华王进甘宜梧谭柏清王绮肖慧包兴艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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