Anaerobe inoculating device, anaerobe culturing system and anaerobe inoculation and culture method

Abstract

The invention relates to an anaerobe inoculating device, an anaerobe culturing system and an anaerobe inoculation and culture method. The anaerobe inoculating device comprises a gas bottle, a pressure meter, a vacuum pump, a vacuum meter and a plurality of groups of inoculation needle components. The technical points are as follows: the gas bottle is filled with inert gas; tee joints are arranged among the gas bottle, the vacuum pump and the inoculation needle components; a master control valve and the pressure meter are arranged between the gas bottle and the tee joints; a ventilating valve and the vacuum meter are arranged between the vacuum pump and the tee joints. The anaerobe culturing system consists of the anaerobe inoculating device, a culture vessel and a soft rubber plug. By utilizing the device and the system, free operation can be implemented under an open condition; the device and the system are applicable to culture of anaerobes in various environments, have the advantages of small size, simplicity in operation and low cost and are remarkable in social benefits and economic benefits and wide in application range.

Description

technical field [0001] The invention relates to an experimental device and a method, in particular to an anaerobic microorganism inoculation device, an anaerobic microorganism cultivation system, and an anaerobic microorganism inoculation and cultivation method. Background technique [0002] At present, most of the culture devices for anaerobic microorganisms refer to anaerobic glove boxes or anaerobic culture tanks. The anaerobic tank cannot realize the anaerobic environment in the process of medium preparation and microbial inoculation, but only ensures the anaerobic environment in the cultivation process, that is, it cannot realize the anaerobic environment in the culture vessel (test tube, triangular flask or culture bottle), and also realizes There is no anaerobic environment required for the growth of anaerobic microorganisms. [0003] Anaerobic glove boxes also have many disadvantages: (1) Inconvenient operation. It is necessary to wear gloves to operate in the incu...

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Problems solved by technology

Therefore, it is necessary to fill the anaerobic glove box with non-oxygen gas, such as nitrogen, carbon dioxide gas, etc. If the oxygen in the air is kept from infiltrating, the anaerobic glove box must be tightly sealed and must not leak, resulting in anaerobic gloves. The production cost of the box is higher

(3) High production cost

(4) Larger volume

(5) Because some strains need to grow under high-pressure conditions, such as strains from seabed and deep soil, but anaerobic glove boxes cannot simulate different pressure conditions, resulting in strains from seabed and soil Inability to grow normally under stress, limited use

(6) It cannot be placed randomly as a whole. Many experiments need to be carried out in a constant temperature incubator. In this way, constant temperature cannot be achieved, so the degree of convenience is not high

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