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System for regenerating gastrointestinal microbiome in vitro and application method of system

A technology of microorganisms and gastrointestinal tract, which is applied in the fields of nutrition, analysis and testing, human physiology, microbiology, and bionics to achieve the effect of avoiding disturbance

Inactive Publication Date: 2019-05-10
SHANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, among the patents applied for gastrointestinal bionic system in China, no one can guarantee the anaerobic environment for sampling, and no one can achieve miniature and simultaneous cultivation of multiple samples to meet the technical requirements of high throughput

Method used

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  • System for regenerating gastrointestinal microbiome in vitro and application method of system
  • System for regenerating gastrointestinal microbiome in vitro and application method of system
  • System for regenerating gastrointestinal microbiome in vitro and application method of system

Examples

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preparation example Construction

[0030] The preparation method of pancreatic juice is as follows: 0.9g trypsin, 6g bile salt, 12.5g NaHCO3 are dissolved in sterile distilled water and fixed to volume with a 1000mL volumetric flask, and the prepared pancreatic juice is stored in a refrigerator at 4°C;

[0031] The preparation method of the food nutrient solution is: 15g Yuexi peptone (Nanjing Maojie Microbial Technology Co., Ltd.), 10g tryptone, 3g soybean peptone, 5g yeast extract powder, 2g beef powder, 13.5g digested serum powder, beef liver extract powder Add 1.2g, glucose 3g, dipotassium hydrogen phosphate 2.5g, sodium chloride 3g, soluble starch 0.3g, L-cysteine ​​0.3g, sodium thioglycolate 0.15g into 1L of distilled water, adjust the pH to 7.2, 25 Sterilize at ℃ for 30 min.

experiment example 1

[0032] Experimental Example 1: Feasibility Evaluation of System Stability

[0033] 1. Research object and method

[0034] Three 10-week-old male rats of SPF grade healthy Kunming species, weighing 300-400 grams, were provided by the Experimental Animal Center of Shanxi University of Traditional Chinese Medicine. The experimental animal production license number: SCXK (Jin) 2018-0002, and the quality of experimental animals was qualified Certificate number: 0081670. The whole experiment was divided into two groups. The first group collected the feces of healthy rats and used the system to reproduce the gastrointestinal microbiome of the rats. The second group directly collected the gastrointestinal contents of the rats after collecting the feces samples. The DNA of the two groups of samples was extracted, the extracted DNA was purified, and then detected by agar gel electrophoresis. The qualified DNA was amplified by PCR, and the quality of the amplified product was tested an...

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Abstract

The invention belongs to the technical field of bionics, human physiology, microbiology, nutrition and analytical testing, and provides a system for regenerating a gastrointestinal microbiome in vitroand an experimental method of the system. The system comprises a sampling device, a simulation device, an injection device and a culture device, wherein the sampling device is a needle tubing sampler; the simulation device comprises an anaerobic tube, the bottom of the anaerobic tube is provided with an ultrafiltration membrane, the anaerobic tube is placed in a centrifuge tube, the top of the anaerobic tube is provided with a butyl rubber stopper, the butyl rubber stopper is provided with a nitrogen catheter and an air catheter, and the nitrogen catheter stretches into the top of the anaerobic tube; the air catheter stretches into the bottom of the anaerobic tube; the injection device is a continuous syringe; the culture device is a constant temperature table concentrator. The simulationsystem is miniature and meets the technical requirements of high throughput. Gastrointestinal simulation is carried out in the same tube, the ultrafiltration membrane is arranged to periodically centrifuge the simulation tube to simulate the absorption of the small intestine, and the effect of simulating the gastrointestinal environment can be achieved.

Description

technical field [0001] The invention belongs to the technical fields of bionics, human physiology, microbiology, nutrition and analysis and testing, and specifically relates to a system for reproducing intestinal microbiome in vitro and an application method thereof. Under in vitro conditions, feces are used to reproduce gastrointestinal A systemic device for the tract microbiome. Because the system device is miniature, it is beneficial for multiple samples to be cultured at the same time, so as to meet the technical requirements of high-throughput reproduction of gastrointestinal microbiome. Background technique [0002] As a part of a complete organism, the intestinal flora is not only involved in maintaining the health of the body, but also closely related to the occurrence and development of many diseases. The intestinal flora is closely related to the physiological and pathological state of the host. Intestinal flora play a very important role in host nutrition, metab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/24C12M1/12C12M1/04C12N1/20
Inventor 胡会玲张希春王菊霞
Owner SHANXI UNIV OF CHINESE MEDICINE
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