The invention discloses a plant tissue culture environmental information monitoring and simulating system. The plant tissue culture environmental information monitoring and simulating system comprises a tissue culture box, a monitoring unit and a simulating unit, wherein the monitoring unit is formed by a collection module, a wireless transmission module and a display processing module. The collection module collects tissue culture environmental information of plants in the tissue culture box, and sends the environmental information to the transmission module. The wireless transmission module uploads the received environmental information to the display processing module. The display processing module stores, processes, displays and indexes the environmental information. The simulating unit carries out manual simulation on the tissue culture environmental information of the plants in the tissue culture box. The plant tissue culture environmental information monitoring and simulating system is convenient to operate, complete in functions, strong in pertinence, capable of achieving intelligent control for the information such as illumination, temperature, carbon dioxide (CO2) and the like in a greenhouse environment, and is suitable for greenhouse plant tissue culture and for various places such as plant tissue culture boxes, illumination culture boxes, phytotrons, facility agriculture, and has expansibility.