Preparation method of Chinese-date probiotics tablet of lactobacillus paracasei capable of producing bile salt hydrolase

A technology of bile salt hydrolysis enzyme and probiotic tablets, applied in the direction of microorganism-based methods, biochemical equipment and methods, lactobacillus, etc., can solve the problem of lowering serum cholesterol and other problems

Inactive Publication Date: 2015-03-25
BEIJING UNIV OF AGRI
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  • Claims
  • Application Information

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Problems solved by technology

Although the above-mentioned patents and documents have reports on the use of probiotics to prepare tablet-type foods, about the use of Lactobacillus paracasei KL1-Liu, which is isolated and screened from the bile salt hydrolyzing enz...

Method used

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  • Preparation method of Chinese-date probiotics tablet of lactobacillus paracasei capable of producing bile salt hydrolase
  • Preparation method of Chinese-date probiotics tablet of lactobacillus paracasei capable of producing bile salt hydrolase
  • Preparation method of Chinese-date probiotics tablet of lactobacillus paracasei capable of producing bile salt hydrolase

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Embodiment 1

[0035] Embodiment 1, the preparation method of bile salt hydrolysis enzyme Lactobacillus paracasei KL1-Liu jujube probiotic sheet

[0036] (1) Activation, expanded culture and high-density fermentation of Lactobacillus paracasei KL1-Liu strain

[0037] The activated Lactobacillus paracasei KL1-Liu strain was enlarged and fermented on the improved MRS medium and fermented at a high density, and the number of viable bacteria obtained was (1.0~5.0)×10 10 CFU / mL of fermentation broth.

[0038] (2) Centrifugal concentration and freeze-drying of Lactobacillus paracasei KL1-Liu bacterial strain

[0039] Centrifuge the fermentation broth at 6000r / min for 10-20min at 4°C, discard the supernatant, and collect the sludge; in a sterilized freeze-dried bottle, add 10.5% ~20.5% maltodextrin + 11.5%~1.5% skimmed milk powder, mix with the collected fungus slime evenly to obtain concentrated starter; pre-freeze the concentrated starter at -40°C for 10 hours until completely frozen to obtain ...

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Abstract

The invention relates to a preparation method of a Chinese-date probiotics tablet of lactobacillus paracasei capable of producing bile salt hydrolase. The preparation method is suitable for producing microecological preparations such as a functional probiotic lactic bacteria living bacteria agent. A formula of the Chinese-date probiotics tablet consists of lactobacillus paracasei KL1-Liu probiotics bacterial powder and auxiliary materials such as Chinese-date powder, fructo-oligosaccharide, xylitol, sucrose, citric acid, microcrystalline cellulose and maltodextrin. The preparation method of the Chinese-date probiotics tablet comprises the following steps: preparing the probiotics bacterial powder from lactobacillus paracasei KL1-Liu CGMCC No.7029 capable of producing the bile salt hydrolase by virtue of processes such as activating, performing enlarged culture, performing high-density fermentation, performing centrifugal concentration, adding a freeze-drying protective additive, pre-freezing, and freeze-drying; mixing the crushed and screened auxiliary materials with the probiotics bacterial powder, thereby obtaining a mixture, and then pressing the mixture into the Chinese-date probiotics tablet. The Chinese-date probiotics tablet prepared by the preparation method disclosed by the invention has good storage stability and a refrigeration living bacteria quality guarantee period of over one year at 4 DEG C, not only can bring the health effects of lowering the serum cholesterol of the products into play, but also can bring the medicinal and edible nutritional and health effects of Chinese-dates into play. Moreover, the preparation method of the Chinese-date probiotics tablet disclosed by the invention has a convenient raw material source, a simple process, a short preparation period and simple operation, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing jujube probiotic tablets that produce bile salt hydrolyzing enzyme and have the effect of lowering serum cholesterol, and are suitable for the production of functional probiotic live lactic acid bacteria preparations (tablet type, powder type) and other microecological preparations. Background technique [0002] The Lactobacillus paracasei (Lactobacillus paracasei) KL1-Liu involved in the present invention is a probiotic lactic acid bacterium that produces bile salt hydrolysis enzyme and has the effects of lowering serum cholesterol and regulating intestinal flora balance, etc. . [0003] Probiotics refer to a class of microorganisms that improve the microecological balance of specific parts of the host through intestinal colonization and have several other beneficial physiological functions. They can improve the ecological balance of intestinal microorganisms, control intestinal infection, reduce serum ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/29A23L1/212A23P1/02C12R1/225A23L19/00A23L33/00
CPCA23V2002/00C12R2001/225C12N1/205A23V2400/125A23V2200/30A23V2200/32A23V2200/326A23V2200/3262A23V2250/032A23V2250/51084A23V2250/5114A23V2250/606A23V2250/628A23V2250/6422
Inventor 刘慧张红星熊利霞张晶金君华高秀芝谢远红
Owner BEIJING UNIV OF AGRI
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