Preparation method of Chinese-date probiotics tablet of lactobacillus paracasei capable of producing bile salt hydrolase
A technology of bile salt hydrolysis enzyme and probiotic tablets, applied in the direction of microorganism-based methods, biochemical equipment and methods, lactobacillus, etc., can solve the problem of lowering serum cholesterol and other problems
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[0035] Embodiment 1, the preparation method of bile salt hydrolysis enzyme Lactobacillus paracasei KL1-Liu jujube probiotic sheet
[0036] (1) Activation, expanded culture and high-density fermentation of Lactobacillus paracasei KL1-Liu strain
[0037] The activated Lactobacillus paracasei KL1-Liu strain was enlarged and fermented on the improved MRS medium and fermented at a high density, and the number of viable bacteria obtained was (1.0~5.0)×10 10 CFU / mL of fermentation broth.
[0038] (2) Centrifugal concentration and freeze-drying of Lactobacillus paracasei KL1-Liu bacterial strain
[0039] Centrifuge the fermentation broth at 6000r / min for 10-20min at 4°C, discard the supernatant, and collect the sludge; in a sterilized freeze-dried bottle, add 10.5% ~20.5% maltodextrin + 11.5%~1.5% skimmed milk powder, mix with the collected fungus slime evenly to obtain concentrated starter; pre-freeze the concentrated starter at -40°C for 10 hours until completely frozen to obtain ...
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