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Efficient cultivating and harvesting method for nitzschia closterium

A harvesting method and technology of diamond algae, applied in biochemical equipment and methods, methods based on microorganisms, single-cell algae, etc., can solve the problems of large infrastructure investment, complicated process, troublesome operation, etc.

Inactive Publication Date: 2015-04-01
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ZBNC culture medium provided by the invention can obtain Nitzschia lunata and its target products by adopting an open culture method, which has obvious effects and stable and reliable operation, but the process is complicated, the operation is troublesome, the infrastructure investment is large, the cost is high, and the benefit is poor

Method used

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  • Efficient cultivating and harvesting method for nitzschia closterium
  • Efficient cultivating and harvesting method for nitzschia closterium

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Embodiment Construction

[0016] In order to overcome the shortcomings of the prior art, an efficient cultivation and harvesting method of Nitzschia lunata is provided. The specific method is as follows:

[0017] Step (1) Preparation of culture medium

[0018] Culture fluid composition: 1 part of cow dung extract, NH 4 VO 3 0.001 parts, CaCl 2 ·2H 2 O 0.1 part, Na 2 MoO 4 0.005 parts, FeC 6 H 5 O 7 0.002 copies, Co(NO 3 ) 2 ·6H 2 O 0.02 parts, human urine 0.8 parts, NaNO 3 0.4 parts, (NH 4 ) 2 SO 4 0.2 part, Na 2 HPO 4 ·2H 2 O 0.1 part, CuSO 4 ·5H 2 O 0.05 parts, EDTA 0.02 parts, FeCl 3 0.001 parts, MgSO 4 ·7H 2 O 0.6 copies, K 2 SiO 3 0.025 parts, indole acetic acid 0.003 parts, sea mud extract 1.2 parts, vitamin H 0.0002 parts, inositol 0.001 parts, folic acid 0.01 parts, thoracic acid 0.004 parts, boiled and cooled seawater 1000 parts; put into a brown ground stopper reagent bottle Medium, seal, place on a shaker and shake for 15 minutes, put in a refrigerator at 4°C for later use;

[0019] Step (2)...

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Abstract

The invention provides an efficient cultivating and harvesting method for nitzschia closterium, and belongs to the field of aquaculture. The nitzschia closterium is mainly extensively cultivated by virtue of an open cement pool in the past, so that the nitzschia closterium is high in cost, easy to pollute, complicated in process, troublesome to operate, large in investment of capital construction and low in yield. According to the efficient cultivating and harvesting method, aerated feeding cultivation is carried out in a microalgae cultivation chamber by virtue of white plastic buckets; and two plastic buckets are connected in series, so that the aerated gas is utilized for the second time, and thus energy and resources are saved. A traditional culture solution formula is also optimized, so that the nitzschia closterium is relatively comprehensive and balanced in nutrient, not easy to pollute, low in cost, and easy to operate; and the yield of the nitzschia closterium is improved by 150%. In addition, a centrifugal process is adopted to harvest the nitzschia closterium in the past, so that the process is high in power consumption, not thorough to participate and high in expense. Due to a compound precipitant, the efficient cultivating and harvesting method is high in sedimentation rate, and thorough to participate; and funds are saved.

Description

[0001] Technical Field The present invention belongs to the field of aquaculture, and in particular relates to an efficient cultivation and harvesting method of Nitzschia small crescent. Background technique [0002] Nitzschia closterium, commonly known as "small diatoms", belongs to the phylum Diatoms, Feathered Diatoms, Tubules, Nitzschiaceae, and Nitzschia genus. It is a warm temperature, salt water species or brackish water Kind of algae. The algae are single-celled, which mainly grow on the beaches of the coastal intertidal zone and also float in the coastal waters, which are found in all sea areas in my country. The center of the cell is enlarged and fusiform. The two ends are tapered, straight or curved in the same direction like a crescent. The cells are 12-23 microns long and 2-3 microns wide. There is a nucleus in the center, the pigment body is yellowish-brown, two pieces, located on both sides of the nucleus, undergo longitudinal division and reproduction. The opti...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王培磊
Owner LINYI UNIVERSITY
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