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Functionalized graphene-based electrochemical sensor arrays

A sensor array, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of long time consumption, development limitations, and high price of sensors, and achieve multiple types of tumors, improve detection performance, and simple instruments Effect

Active Publication Date: 2017-07-28
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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Problems solved by technology

However, screening specific ligands (such as ligand system evolution by exponential enrichment in vitro, SELEX) and constructing special sensors are often time-consuming and expensive, and require a certain understanding of the specific biomarkers used
Development in this field has been severely limited by the difficulty of finding a specific marker that can differentiate between cancer cells or between specific cancer cells and their metastatic status

Method used

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  • Functionalized graphene-based electrochemical sensor arrays
  • Functionalized graphene-based electrochemical sensor arrays
  • Functionalized graphene-based electrochemical sensor arrays

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preparation example Construction

[0043] (3) Preparation of electrochemical sensor array:

[0044] Glassy carbon electrodes with a diameter of 3 mm were first ground on alumina polishing powders with a particle size of 1.0, 0.3, and 0.05 μm, and the electrodes were ultrasonically cleaned in ultrapure water for 3 minutes after each step of grinding. 10 μL of the BSA-stabilized reduced graphene probe prepared in step (1) and 10 μL of the calf thymus DNA functionalized reduced graphene probe dispersion prepared in step (2) were dropped onto the pretreated electrode surface and dried at room temperature spare. The glassy carbon electrodes can also be replaced by ITO or screen printing electrodes.

[0045] Detection of cells with fabricated functionalized graphene-based electrochemical sensor arrays:

[0046] (1) Cell culture and treatment:

[0047] Cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum, penicillin and streptomycin (100 μg / mL) in humid 5% CO 2 Incubate at 37°C. After culturi...

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Abstract

The invention relates to an electrochemical sensor array based on functionalized graphene. The electrochemical sensor array comprises: an electrode, and a BSA-stabilized reduced graphene probe modified on the electrode surface and a calf thymus DNA functionalized reduced graphene probe; the concentration of the BSA-stabilized reduced graphene probe The concentration of the reduced graphene probe functionalized with the calf thymus DNA was the same. The sensor array platform can realize the distinction of different cancer cells and different states of the same cancer cell, such as drug-resistant breast cancer cells and metastatic breast cancer cell samples. Designing the biomimetic interaction between the sensor recognition unit and the cell and combining it with an ultra-high sensitivity electrochemical method significantly improves the detection performance of the sensor, and can achieve the distinction of different types of cells at the level of 100 cells, detection The accuracy can reach 100%, and the detection limit for a single type of cancer cell can reach the single cell level.

Description

technical field [0001] The invention relates to an electrochemical sensor, in particular to an electrochemical sensor array based on functionalized graphene. Background technique [0002] Although a lot of energy and scientific research funds have been invested in cancer research all over the world, cancer is still one of the main causes of death and brings huge losses to society and economy. Although many advanced cancer treatment methods have been developed in the past decades, early diagnosis of cancer is still the key to cancer treatment. Rapid diagnosis and early intervention before the cancer spreads can control the development of the disease in time, which can not only reduce cancer mortality, improve the quality of life of patients, but also reduce medical expenses. The early detection of cancer is usually aimed at the analysis of tumor markers, which are indicative chemical substances to evaluate the occurrence and development of cancer and the effect of drug treat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/26
Inventor 曲晓刚吴丽关意佳冉翔
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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