Improved diluent for long-term preservation of semen of ruminant

A ruminant and diluent technology, applied in the diluent field, can solve the problems of insufficient sperm motility and high cost, and achieve the effects of improving sperm motility, reducing damage and improving stability

Inactive Publication Date: 2015-05-06
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention has achieved good effect on cryopreservation and cryopreservation of dairy goat semen, but the semen dilution disclosed in this invention exists due to the abundant unsaturated

Method used

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  • Improved diluent for long-term preservation of semen of ruminant
  • Improved diluent for long-term preservation of semen of ruminant

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Example 1

[0015] Combine 100 mL of double distilled water, 3 g of trehalose, 20 mL of egg yolk, 10 mg of superoxide dismutase, 4 g of fructose, 6 g of trihydroxyaminomethane, 1.5 g of citric acid, 50 mg of glutathione, 180 mg of bovine serum albumin, 50 mg of adenosine triphosphate, and vitamins. C 800mg, 800,000 units of penicillin, and 100,000 units of streptomycin can be mixed evenly.

Example Embodiment

[0016] Example 2

[0017] Combine 100 mL of double-distilled water, 2 g of trehalose, 25 mL of egg yolk, 5 mg of superoxide dismutase, 1 g of fructose, 8 g of trihydroxyaminomethane, 2.5 g of citric acid, 70 mg of glutathione, 100 mg of bovine serum albumin, 100 mg of adenosine triphosphate, and vitamins. Mix C300mg, 500,000 units of penicillin, and 200,000 units of streptomycin.

Example Embodiment

[0018] Example 3

[0019] Combine 100 mL of double distilled water, 3 g of trehalose, 15 mL of egg yolk, 7 mg of superoxide dismutase, 2 g of fructose, 4 g of trihydroxyaminomethane, 2.5 g of citric acid, 30 mg of glutathione, 220 mg of bovine serum albumin, 100 mg of adenosine triphosphate, and vitamins. C 500mg, 300,000 units of penicillin, and 200,000 units of streptomycin can be mixed evenly.

[0020] In order to study the effect of the present invention, take the diluent of Example 1 as an example. Observe the collected semen through experiments in the laboratory and determine the viability and density. Use 0.7 and 0.6 as the standard to calculate the percentage of semen with excellent, good and standard sperm motility. Select semen with viability ≥ 0.7, determine the dilution factor, dilute the semen with the diluent of the present invention, store at 0°C, thawed in a 35°C water bath for 20 seconds, microscopic examination, semen with a viability of 0.5 or more for infusion ...

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Abstract

The invention discloses improved diluent for long-term preservation of semen of ruminant. The improved diluent is characterized in that per 100ml of double distilled water contains 3 to 9g of tris (hydroxymethyl) aminomethane, 5 to 25ml of yolk, 0.5 to 3g of trehalose, 1 to 4g of fructose, 10 to 90mg of glutathione, 60 to 280mg of bovine serum albumin, 20 to 150mg of triphosadenine, 100 to 800mg of vitamin C, 0.8 to 2.8g of citric acid, 100000 to 800000 units of penicillin, 50000 to 300000 units of streptomycin, and 2 to 10mg of superoxide dismutase. The diluents has a good effect on preserving semen of ruminants at low temperature, such as sheep, cattle and deer; in addition, the production method is simple, the production cost is low, and the popularization and application are convenient.

Description

technical field [0001] The invention relates to a diluent for long-term preservation of semen in ruminant artificial insemination technology, in particular to a diluent for long-term preservation of semen of sheep, cattle and deer. Background technique [0002] Ruminants such as camels, deer, alpacas, antelopes, cattle, and sheep are important economic animals in my country. These ruminants have extremely high meat, milk and medicinal values. At present, artificial insemination technology is mostly used in large-scale and retail breeding to speed up the improvement of ruminant species, increase the breeding speed and establish a large-scale breeding management system. [0003] Semen cryopreservation is one of the key technologies of artificial insemination. The use of frozen semen for artificial insemination can not be limited by time, region, variety and individual, and it can greatly reduce the number of male animals, reduce production costs, and increase the utilization r...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 高庆华卢全晟邹宇银马梦婷韩春梅
Owner TARIM UNIV
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