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Improved diluent for long-term preservation of semen of ruminant

A ruminant and diluent technology, applied in the diluent field, can solve the problems of insufficient sperm motility and high cost, and achieve the effects of improving sperm motility, reducing damage and improving stability

Inactive Publication Date: 2015-05-06
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This invention has achieved good effect on cryopreservation and cryopreservation of dairy goat semen, but the semen dilution disclosed in this invention exists due to the abundant unsaturated fatty acids on the sperm plasma membrane that maintain membrane fluidity, which are very easy to store under aerobic conditions Insufficient sperm motility and high cost caused by oxidation

Method used

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  • Improved diluent for long-term preservation of semen of ruminant
  • Improved diluent for long-term preservation of semen of ruminant

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Double distilled water 100mL and trehalose 3g, egg yolk 20mL, superoxide dismutase 10mg, fructose 4g, trishydroxyaminomethane 6g, citric acid 1.5g, glutathione 50mg, bovine serum albumin 180mg, adenosine triphosphate 50mg, vitamin Mix C 800mg, penicillin 800,000 units, and streptomycin 100,000 units evenly.

Embodiment 2

[0017] Double distilled water 100mL and trehalose 2g, egg yolk 25mL, superoxide dismutase 5mg, fructose 1g, trishydroxyaminomethane 8g, citric acid 2.5g, glutathione 70mg, bovine serum albumin 100mg, adenosine triphosphate 100mg, vitamin Mix C300mg, penicillin 500,000 units, and streptomycin 200,000 units evenly.

Embodiment 3

[0019] Double distilled water 100mL and trehalose 3g, egg yolk 15mL, superoxide dismutase 7mg, fructose 2g, trishydroxyaminomethane 4g, citric acid 2.5g, glutathione 30mg, bovine serum albumin 220mg, adenosine triphosphate 100mg, vitamin Mix C 500mg, penicillin 300,000 units, and streptomycin 200,000 units evenly.

[0020] In order to study the effect of the present invention, the dilution of Example 1 is taken as an example. In the laboratory, observe the collected semen and measure the motility and density, and count the percentages of semen with excellent, good and standard sperm motility with 0.7 and 0.6 as the standard. Select the semen with a viability ≥ 0.7, determine the dilution factor, dilute the semen with the diluent of the present invention, store it in an environment at 0°C, thaw it in a water bath at 35°C for 20 seconds, and perform microscopic examination. The semen with a viability of 0.5 or more is used for insemination .

[0021] At the same time, the comm...

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Abstract

The invention discloses improved diluent for long-term preservation of semen of ruminant. The improved diluent is characterized in that per 100ml of double distilled water contains 3 to 9g of tris (hydroxymethyl) aminomethane, 5 to 25ml of yolk, 0.5 to 3g of trehalose, 1 to 4g of fructose, 10 to 90mg of glutathione, 60 to 280mg of bovine serum albumin, 20 to 150mg of triphosadenine, 100 to 800mg of vitamin C, 0.8 to 2.8g of citric acid, 100000 to 800000 units of penicillin, 50000 to 300000 units of streptomycin, and 2 to 10mg of superoxide dismutase. The diluents has a good effect on preserving semen of ruminants at low temperature, such as sheep, cattle and deer; in addition, the production method is simple, the production cost is low, and the popularization and application are convenient.

Description

technical field [0001] The invention relates to a diluent for long-term preservation of semen in ruminant artificial insemination technology, in particular to a diluent for long-term preservation of semen of sheep, cattle and deer. Background technique [0002] Ruminants such as camels, deer, alpacas, antelopes, cattle, and sheep are important economic animals in my country. These ruminants have extremely high meat, milk and medicinal values. At present, artificial insemination technology is mostly used in large-scale and retail breeding to speed up the improvement of ruminant species, increase the breeding speed and establish a large-scale breeding management system. [0003] Semen cryopreservation is one of the key technologies of artificial insemination. The use of frozen semen for artificial insemination can not be limited by time, region, variety and individual, and it can greatly reduce the number of male animals, reduce production costs, and increase the utilization r...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 高庆华卢全晟邹宇银马梦婷韩春梅
Owner TARIM UNIV
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