An offshore escherichia coli detection method

A technology of Escherichia coli and detection method, applied in the field of coastal Escherichia coli detection, can solve the problems of long detection period, low sensitivity, cumbersomeness and the like, and achieve the effects of low cost and high sensitivity

Inactive Publication Date: 2015-05-06
大连大公检验检测有限公司
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Problems solved by technology

However, this method is very cumbersome, requires a lot of manpower and material resources, and the detection cycle is long

Method used

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Embodiment 1

[0010] The detection method of Escherichia coli near the coast uses a suction filtration system to trap Escherichia coli in seawater on the filter membrane, and then place the filter membrane in the medium for cultivation. The filter membrane is a microporous filter membrane with a pore size of 0.45 μm, and the detection solution is added The enrichment operation was carried out in the bile salt lactose enrichment solution, and the enrichment was carried out for 30-48 hours. If it was turbid and gas produced, it was determined that Escherichia coli was detected. The culture medium is an eosin-methylene blue agar plate, and the culture time is 10-12 days. After culturing, observe the colony growth of Escherichia coli. . The membrane filtration is performed by a centrifugal double membrane filtration method. The filtration centrifugal radius of the method is 20cm, and the rotating speed is 4000 rpm.

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Abstract

An offshore escherichia coli detection method is provided. According to the method, escherichia coli in seawater is intercepted onto a filter membrane by a suction filtration system, the filter membrane is put into a culture medium and cultured and is a microfiltration membrane having a pore diameter of 0.45 [mu]m, a bile lactose enrichment broth is added into a detection solution to perform bacteria-proliferating operation for 30-48 h, and if the solution is muddy and a gas is generated, it is determined that the escherichia coli is detected. The culture medium is an eosin-methylene blue agar plate, the culture time is 10-12 d, and colony growth conditions of the escherichia coli are observed after culturing. If a typical escherichia coli colony grows and is separated from the eosin-methylene blue agar plate, it is determined that the escherichia coli are detected. Compared with traditional microbe detection methods, the method is low in cost, rapid, convenient, simple, and high in sensitivity.

Description

technical field [0001] The invention relates to the technical field of detection of common bacteria, in particular to a detection method for near-coast Escherichia coli. Background technique [0002] Escherichia coli (Escherichia coli), classified in Enterobacteriaceae, belongs to the genus Escherichia, and Escherichia coli related to human diseases generally includes five species, namely enterotoxic Escherichia coli (ETEC), pathogenic Escherichia coli (SPEC ), hemorrhagic Escherichia coli (EHEC), invasive Escherichia coli (EIEC) and adherent Escherichia coli (EAEC). Escherichia coli is often excreted from human and animal bodies with feces and spread widely in nature. Therefore, once Escherichia coli is detected, it means that it is directly or indirectly contaminated by feces, and it is used in hygiene as a hygienic bacteriological indicator of fecal contamination of drinking water, milk or food; Some major enteropathogens are similar, and its appearance may also indicat...

Claims

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Application Information

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IPC IPC(8): C12Q1/10
Inventor 关杰关宇江美玲曹卉王清
Owner 大连大公检验检测有限公司
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