Ailanthus altissima bark algistat and preparation method and application thereof

A technology of Ailanthus bark and algae inhibitor, which is applied in the field of Ailanthus bark and its preparation, can solve the problems of high technical requirements for the separation and extraction of allelochemicals, affecting the allelopathic potential of aquatic plants, and the growth limitation of aquatic plants, etc., to achieve Excellent anti-algae performance, simple preparation method and high inhibition efficiency

Inactive Publication Date: 2015-05-13
TAISHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth of aquatic plants is severely restricted by seasons and climates. At the same time, the release of toxic substances such as algae toxins will affect the allelopathic potential of aquatic plants.
In addition,

Method used

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  • Ailanthus altissima bark algistat and preparation method and application thereof
  • Ailanthus altissima bark algistat and preparation method and application thereof
  • Ailanthus altissima bark algistat and preparation method and application thereof

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Experimental program
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Effect test

Example Embodiment

[0029] Example 1 Preparation method of algae inhibitor of the present invention

[0030] (1) Wash the bark of Ailanthus altissima and place it in an oven at 70-80℃ to dry for later use.

[0031] (2) The dried bark of Ailanthus altissima is crushed with a pulverizer and passed through a 100-mesh sieve.

[0032] (3) Weigh 15-20g of Ailanthus altissima dry powder, and extract with organic solvents according to the Soxhlet extraction method, the extraction temperature is 60-80°C, and the extraction time is 5-6h. The organic solvent is absolute ethanol.

[0033] (4) The extract obtained by Soxhlet extraction is placed in a rotary evaporator, and the ethanol is evaporated under reduced pressure, concentrated, and dried to constant weight to obtain a crude ethanol extract of Ailanthus altissima bark.

Example Embodiment

[0034] Example 2 Application of the algae inhibitor of the present invention

[0035] Experimental Materials:

[0036] Microcystis aeruginosa (M.aeruginosa FACHB-905, provided by the Freshwater Algae Species Bank of Chinese Academy of Sciences).

[0037] The BG11 medium formula is:

[0038] NaNO 3 1.5g / L, K 2 HPO 4 40mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 ·2H 2 O 36mg / L, Citric acid 6mg / L, Ferric ammonium citrate 6mg / L, EDTANa 2 1mg / L, Na 2 CO 3 20mg / L, A 5 Solution 1mL / L. Where A 5 Solution formula: MnCl 2 ·4H 2 O 1.86g / L, ZnSO 4 ·7H 2 O 0.22g / L, Na 2 MoO 4 ·2H 2 O 0.39g / L, H 3 BO 3 2.86g / L, CuSO 4 ·5H 2 O 0.08g / L, Co(NO 3 ) 2 ·6H 2 O 0.05g / L.

[0039] The expansion of Microcystis aeruginosa was carried out before the experiment. The culture conditions are: photoperiod 14h / 10h, temperature (25±1)℃, shaking 3 to 5 times a day, so that Microcystis aeruginosa enters the logarithmic growth phase.

[0040] Use the crude extract of Ailanthus altissima bark to conduct toxicity test on aseptical...

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Abstract

The invention discloses an ailanthus altissima bark algistat and a preparation method and an application thereof, and belongs to the field of water boy pollution control. The algistat is an absolute ethyl alcohol crude extract of ailanthus altissima bark. Compared with the prior art, the defects that an existing chemical method causes secondary pollution and is high in cost, unlikely to be controlled, likely to damage the ecological environment and the like are overcome, and the limitation that the algistat is high in technical cost and complicated in operation is solved by utilizing water plant extraction. The algistat is extracted from the woody plant ailanthus altissima bark, and the preparation method is simple, convenient to use and high in operability and is suitable for large-scale popularization. The prepared algistat has high inhibition efficiency for microcystis aeruginosa, and can become effective on the second day; the inhibition ratio reaches more than 90%, and the water body can be effectively prevented from breaking out water bloom for a long period of time.

Description

technical field [0001] The invention belongs to the field of water body pollution control, and in particular relates to an algae-inhibiting agent of Ailanthus bark, its preparation method and application. Background technique [0002] With the development of economy and the increase of human activities, the eutrophication of lakes and reservoirs is becoming more and more serious. Eutrophication of water body leads to frequent outbreaks of cyanobacteria blooms, which not only affects water quality, but also releases harmful toxins, seriously endangering aquatic organisms and even human health. It has become a serious problem facing the current global ecological environment. Therefore, how to effectively control the excessive growth of harmful cyanobacteria, Then preventing algae blooms from happening has become a research focus and frontier in the field of environment. [0003] The current algae control technologies mainly include the following: physical methods such as isol...

Claims

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Application Information

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IPC IPC(8): C02F1/50
CPCC02F1/50C02F2303/20
Inventor 孟盼盼刘忠德高廷东
Owner TAISHAN UNIV
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