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Ailanthus altissima bark algistat and preparation method and application thereof

A technology of Ailanthus bark and algae inhibitor, which is applied in the field of Ailanthus bark and its preparation, can solve the problems of high technical requirements for the separation and extraction of allelochemicals, affecting the allelopathic potential of aquatic plants, and the growth limitation of aquatic plants, etc., to achieve Excellent anti-algae performance, simple preparation method and high inhibition efficiency

Inactive Publication Date: 2015-05-13
TAISHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth of aquatic plants is severely restricted by seasons and climates. At the same time, the release of toxic substances such as algae toxins will affect the allelopathic potential of aquatic plants.
In addition, the separation and extraction of allelochemicals from aquatic plants has problems such as high technical requirements and high cost, so the use of aquatic plants to inhibit algae has certain limitations in practical applications

Method used

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  • Ailanthus altissima bark algistat and preparation method and application thereof
  • Ailanthus altissima bark algistat and preparation method and application thereof
  • Ailanthus altissima bark algistat and preparation method and application thereof

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Effect test

Embodiment 1

[0029] The preparation method of embodiment 1 algastatic agent of the present invention

[0030] (1) Wash the Ailanthus bark and dry it in an oven at 70-80°C for later use.

[0031] (2) The dried Ailanthus bark is pulverized with a pulverizer and passed through a 100-mesh sieve.

[0032] (3) Weigh 15-20 g of Ailanthi bark dry powder, and extract it with an organic solvent according to the Soxhlet extraction method, the extraction temperature is 60-80° C., and the extraction time is 5-6 hours. Described organic solvent is dehydrated alcohol.

[0033] (4) The extract obtained by Soxhlet extraction is placed in a rotary evaporator, and the ethanol is evaporated to dryness by decompression and concentration, and dried to a constant weight to obtain an anhydrous ethanol crude extract of Ailanthus bark.

Embodiment 2

[0034] Embodiment 2 The application of algastatic agent of the present invention

[0035] Experimental Materials:

[0036] Microcystis aeruginosa (M.aeruginosa FACHB-905, provided by the Freshwater Algae Species Bank of the Chinese Academy of Sciences).

[0037] The formula of BG11 medium is:

[0038] NaNO 3 1.5g / L, K 2 HPO 4 40mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 36mg / L, Citric acid 6mg / L, Ferric ammonium citrate 6mg / L, EDTANa 2 1mg / L, Na 2 CO 3 20mg / L, A 5 Solution 1mL / L. where A 5 The solution formula is: MnCl 2 4H 2 O 1.86g / L, ZnSO 4 ·7H 2 O 0.22g / L, Na 2 MoO 4 2H 2 O 0.39g / L, H 3 BO 3 2.86g / L, CuSO 4 ·5H 2 O 0.08g / L, Co(NO 3 ) 2 ·6H 2 O 0.05g / L.

[0039] Microcystis aeruginosa was expanded and cultured before the experiment. The culture conditions are: photoperiod 14h / 10h, temperature (25±1)°C, shaking 3-5 times a day, so that Microcystis aeruginosa enters logarithmic growth phase.

[0040] Use the absolute ethanol crude extract of Aila...

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Abstract

The invention discloses an ailanthus altissima bark algistat and a preparation method and an application thereof, and belongs to the field of water boy pollution control. The algistat is an absolute ethyl alcohol crude extract of ailanthus altissima bark. Compared with the prior art, the defects that an existing chemical method causes secondary pollution and is high in cost, unlikely to be controlled, likely to damage the ecological environment and the like are overcome, and the limitation that the algistat is high in technical cost and complicated in operation is solved by utilizing water plant extraction. The algistat is extracted from the woody plant ailanthus altissima bark, and the preparation method is simple, convenient to use and high in operability and is suitable for large-scale popularization. The prepared algistat has high inhibition efficiency for microcystis aeruginosa, and can become effective on the second day; the inhibition ratio reaches more than 90%, and the water body can be effectively prevented from breaking out water bloom for a long period of time.

Description

technical field [0001] The invention belongs to the field of water body pollution control, and in particular relates to an algae-inhibiting agent of Ailanthus bark, its preparation method and application. Background technique [0002] With the development of economy and the increase of human activities, the eutrophication of lakes and reservoirs is becoming more and more serious. Eutrophication of water body leads to frequent outbreaks of cyanobacteria blooms, which not only affects water quality, but also releases harmful toxins, seriously endangering aquatic organisms and even human health. It has become a serious problem facing the current global ecological environment. Therefore, how to effectively control the excessive growth of harmful cyanobacteria, Then preventing algae blooms from happening has become a research focus and frontier in the field of environment. [0003] The current algae control technologies mainly include the following: physical methods such as isol...

Claims

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Application Information

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IPC IPC(8): C02F1/50
CPCC02F1/50C02F2303/20
Inventor 孟盼盼刘忠德高廷东
Owner TAISHAN UNIV
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