Ailanthus altissima bark algistat and preparation method and application thereof
A technology of Ailanthus bark and algae inhibitor, which is applied in the field of Ailanthus bark and its preparation, can solve the problems of high technical requirements for the separation and extraction of allelochemicals, affecting the allelopathic potential of aquatic plants, and the growth limitation of aquatic plants, etc., to achieve Excellent anti-algae performance, simple preparation method and high inhibition efficiency
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[0029] Example 1 Preparation method of algae inhibitor of the present invention
[0030] (1) Wash the bark of Ailanthus altissima and place it in an oven at 70-80℃ to dry for later use.
[0031] (2) The dried bark of Ailanthus altissima is crushed with a pulverizer and passed through a 100-mesh sieve.
[0032] (3) Weigh 15-20g of Ailanthus altissima dry powder, and extract with organic solvents according to the Soxhlet extraction method, the extraction temperature is 60-80°C, and the extraction time is 5-6h. The organic solvent is absolute ethanol.
[0033] (4) The extract obtained by Soxhlet extraction is placed in a rotary evaporator, and the ethanol is evaporated under reduced pressure, concentrated, and dried to constant weight to obtain a crude ethanol extract of Ailanthus altissima bark.
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[0034] Example 2 Application of the algae inhibitor of the present invention
[0035] Experimental Materials:
[0036] Microcystis aeruginosa (M.aeruginosa FACHB-905, provided by the Freshwater Algae Species Bank of Chinese Academy of Sciences).
[0037] The BG11 medium formula is:
[0038] NaNO 3 1.5g / L, K 2 HPO 4 40mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 ·2H 2 O 36mg / L, Citric acid 6mg / L, Ferric ammonium citrate 6mg / L, EDTANa 2 1mg / L, Na 2 CO 3 20mg / L, A 5 Solution 1mL / L. Where A 5 Solution formula: MnCl 2 ·4H 2 O 1.86g / L, ZnSO 4 ·7H 2 O 0.22g / L, Na 2 MoO 4 ·2H 2 O 0.39g / L, H 3 BO 3 2.86g / L, CuSO 4 ·5H 2 O 0.08g / L, Co(NO 3 ) 2 ·6H 2 O 0.05g / L.
[0039] The expansion of Microcystis aeruginosa was carried out before the experiment. The culture conditions are: photoperiod 14h / 10h, temperature (25±1)℃, shaking 3 to 5 times a day, so that Microcystis aeruginosa enters the logarithmic growth phase.
[0040] Use the crude extract of Ailanthus altissima bark to conduct toxicity test on aseptical...
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