Earthstar scleroderma strain, and culture medium, culture method and application thereof

A hard-skinned puffball and culture medium technology, applied in microorganism-based methods, applications, biochemical equipment and methods, etc., can solve the problems of depletion of planting resources and scarcity of wild resources, and achieve more metabolites and secondary metabolites. High content and strong vitality

Active Publication Date: 2015-05-13
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since the star-cracked puffball is a kind of delicacy and has certain medicinal effects, the market demand for the star-cracked puffball is increasing. However, wild resources are extremely scarce, and a large number of harvesting in recent years has caused its planting resources to be on the verge of exhaustion.

Method used

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  • Earthstar scleroderma strain, and culture medium, culture method and application thereof
  • Earthstar scleroderma strain, and culture medium, culture method and application thereof
  • Earthstar scleroderma strain, and culture medium, culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] The production of embodiment 1 seed

[0081] (1) Inclined bacteria:

[0082] Medium: PDA medium glucose 2%, potato 20%, agar 2%, pH natural.

[0083] Inoculate the PDA culture medium with the preservation number of CGMCC No.7406 Scleroderma starella puffball strain, culture temperature 23 ℃ ~ 25 ℃, 70 hours mycelia covered the slope, put it in the refrigerator (2 ~ 4 ℃) for future use.

[0084] (2) secondary shake flask strains

[0085] Medium: sucrose 3%, peptone 2%, KH 2 SO 4 0.1%, MgSO 4 0.1%, natural pH. Sterilized for later use.

[0086] Take the slant strains with short bacterial age and thick and dense hyphae, and insert them into the sterilized shake flask culture medium (according to the ratio of 1:6, that is, 1 slant connects 6 shake flasks) under aseptic conditions, at 23°C ~25°C temperature-controlled culture, shaking flask rotation speed 160-220r / min (rotary type), when the fermentation liquid per 100ml obtains a mycelium dry weight of more than 1.2g...

Embodiment 2

[0090] The production of embodiment 2 seeds

[0091] (1) Inclined bacteria:

[0092] Medium: PDA medium glucose 2%, potato 20%, agar 2%, pH natural.

[0093] Inoculate the PDA culture medium with the preservation number of CGMCC No.7046 Puffball strains star cracked, culture temperature 23 ℃ ~ 28 ℃, 71 hours mycelium covered the slope, put it in the refrigerator (2 ~ 4 ℃) for future use.

[0094] (2) secondary shake flask strains

[0095] Medium: 2% to 3% sucrose, 1% to 2% peptone, KH 2 SO 4 0.1%~0.15%, 0.1%~0.15%MgSO 4 , natural pH. Sterilized for later use.

[0096] Take the slant strains with short bacterial age and thick and dense hyphae, and insert them into the sterilized shake flask culture medium (according to the ratio of 1:6, that is, 1 slant connects 6 shake flasks) under aseptic conditions, at 23°C Temperature-controlled culture at ~28°C, shaker flask rotation speed 160-220r / min (rotary type), when the fermentation liquid per 100ml obtains a mycelium dry wei...

Embodiment 3

[0100] The production of embodiment 3 seeds

[0101] (1) Inclined bacteria:

[0102] Medium: PDA medium glucose 2%, potato 20%, agar 2%, pH natural.

[0103] Inoculate the PDA culture medium with the preservation number of CGMCC No.7046. The puffball strains are inoculated on PDA medium, cultivated at a temperature of 22°C to 25°C, and the mycelia cover the slope within 72 hours, and then stored in a refrigerator (2 to 4°C) for later use.

[0104] (2) secondary shake flask strains

[0105] Medium: glucose 2%~3%, yeast powder 1%~2%, KH 2 SO 4 0.1%~0.15%, 0.1%~0.15%MgSO 4 , natural pH. Sterilized for later use.

[0106] Take the slant strains with short bacterial age and strong and dense hyphae, and insert them into the sterilized shake flask culture medium (according to the ratio of 1:6, that is, 1 slant connects 6 shake flasks) under aseptic conditions, at 22°C ~25°C temperature-controlled culture, shaking flask rotation speed 160-220r / min (rotary type), when the fermen...

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Abstract

The invention relates to the field of fungus cultivation and the field of fermentation of fungus liquid, in particular to an earthstar scleroderma strain, and a culture medium, a culture method and application thereof. The invention provides the earthstar scleroderma strain with the preservation number of CGMCC No.7406, provides a seed culture medium, the seed culture method, a liquid fermentation method and the culture medium of the strain and also provides application of bacterium solution (fermentative hyphae and fermentation liquor) obtained after strain liquid is fermented. The strain provided by the invention has excellent stability and strong metabolic capability and is not aged or degenerated after being subjected to passage to the 25th generation. When the liquid culture medium and the fermentation method (namely the culture method) which are provided by the invention are adopted to prepare the fermentative hyphae, the hyphae grow rapidly, are uniform and are branched a little and rich metabolic products are generated.

Description

technical field [0001] The invention relates to the field of fungal liquid fermentation, in particular to a star cracked puffball strain and its culture medium, culture method and application. Background technique [0002] The star-cracking puffball is the fruiting body of the fungus Scleroderma polyrhizum Pers. Born in sandy land, grassy soil slopes and forest margins. Visible in summer and autumn. It is an ectomycorrhizal fungus and forms mycorrhizae with a variety of coniferous and broad-leaved trees. The mycorrhizal roots are white when young and brown when old. Distributed in Jilin, Shandong, Jiangsu, Zhejiang, Henan, Hunan, Sichuan, Guizhou, Yunnan, Tibet and other places. Star-cracked hard-skinned puffball fruiting body spherical, 4-8cm in diameter. Juveniles are pure white, later light khaki, with stripes or scales on the surface, star-shaped cracks after maturity. There are mycelium discs at the base, branched out into many bunches of roots. The spores are dar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L2/38A23L1/29A23L1/28A61K36/07A61P7/04A61P29/00A61P11/04C12R1/645A23L31/00
CPCA23L2/52A23L31/00A23L33/135A61K36/07C12N1/14C12R2001/645C12N1/145
Inventor 王淑敏宫诗林杨彧秀相坤周贤马春华
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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