Introduction and breeding method for Mulinia lateralis Say
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A dwarf and artificial breeding technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of no natural distribution, lack of artificial breeding technology, no artificial breeding of dwarf clams, etc.
Pending Publication Date: 2022-02-11
OCEAN UNIV OF CHINA
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[0003] The research on dwarf clams can be traced back to the 1950s. However, due to the lack of effective artificial breeding technology, the research objects are mainly wild populations, but there is no natural distribution in China, and there is no domestic artificial breeding of dwarf clams. clam report
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Embodiment 1
[0031] Embodiment 1 parental maturation promotion and induced labor hatching
[0032] Select 500 adults of the second-generation population of dwarf clams with a shell length of about 3-5 mm for artificial breeding. The adult dwarf clams selected are as follows: figure 1 shown.
[0033] 1. Parental maturation:
[0034] Put the selected dwarf clam individuals into the parental maturation pond, and use circulating water for breeding, such as figure 2 As shown, or the circulation system commonly used in aquaculture; the specific operation is as follows:
[0035] Parental ripening pond: wash the pond with fresh water first (including the trachea, air stone and pool plug), then fill it up with fresh water, add 10ml 84 disinfectant and soak for at least 6 hours, after the end, scrub carefully with fresh water twice, and then Rinse it with sea water, and finally add about 4 / 5 of sea water and supply oxygen.
[0036] Adding of attachment base (sand): dwarf clam is a buried type s...
Embodiment 2
[0047] Embodiment 2: larva cultivation:
[0048] Selection of the best: After the hatching process, poor quality larvae and unsuccessfully developed fertilized eggs will sink to the bottom of the glass tank. The shell length of D-shaped larvae of dwarf clams is generally less than 60um, so the 400-mesh sieve is used to filter and collect the floating larvae, and transfer them to a new sterilized glass tank, add filtered seawater at 24°C, write a label, and sink. Discard all of the bottom. In addition, the selected tools need to be sterilized in advance
[0049] Larva cultivation: After selection, it enters the stage of larva cultivation, where the larvae are small and have poor adaptability to the environment. They have high requirements for water quality and must be carefully managed. Every day, observe changes in room temperature, water temperature, and water color, as well as the feeding and growth status of the larvae, whether the inflation is normal, and the development...
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Abstract
The invention discloses an introduction and breeding method of the Mulinia lateralis Say. Introduction and breeding of the Mulinia lateralis Say in China are successfully achieved, control over the breeding period of the Mulinia lateralis Say can be successfully achieved, and the period is controlled to be about two months and is equivalent to the fastest growth speed of the Mulinia lateralis Say under the natural condition. According to the method, rapid and large-batch breeding of the Mulinia lateralis Say is achieved, and it can be guaranteed that materials of the Mulinia lateralis Say in all development stages are obtained. In addition, compared with the Mulinia lateralis Say under natural conditions, the survival rate of the Mulinia lateralis Say is high, and the growth and development speed of larvae and adults is high. During the whole breeding period, the temperature is always maintained at about 23 DEG C so as to ensure the rapid growth and development of the Mulinia lateralis Say; meanwhile, the temperature is reduced by 2-3 DEG C in the maturation promoting stage to stimulate rapid development of the gonads, the growth and development speed is not affected by too low temperature, and meanwhile, the development of the gonads of the parent shellfish is promoted by increasing the oxygenation amount and reducing the breeding density.
Description
technical field [0001] The invention belongs to the technical field of marine organism breeding, and relates to a method for introducing and breeding dwarf clams. Background technique [0002] The dwarf clam (Mulinia lateralis) is naturally distributed in the Atlantic coast of North America and the Caribbean Sea. It belongs to the mollusk phylum, clam order, clam family, and is a small bivalve buried mollusk. It has the characteristics of short development and reproduction cycle (about 2 months), small size, and transparent shell in early development. As one of the three main animal groups on the earth, the crown chakra to which molluscs belong, represents the main animal species in the ocean. However, the corresponding terrestrial animals have mature model animal systems, such as mice, fruit flies, zebrafish, etc., and molluscs have no model organisms so far; and the potential of dwarf clams as model shellfish is recognized by the international community. [0003] The res...
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