A kind of preparation method of artificial cornea

A technology for artificial corneas and corneas, applied in the direction of eye implants, etc., can solve the problems of low enzymatic hydrolysis efficiency, inability to ensure, and inability to achieve decellularization effects, etc., to achieve the effect of promoting repair and reconstruction

Active Publication Date: 2019-04-26
XIAMEN DAKAI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Fifth, the DNA-RNA enzyme used cannot ensure that the enzyme can act on the specific position of the corneal cells relatively accurately, and the enzymatic hydrolysis efficiency is low. Therefore, a relatively ideal decellularization effect cannot be achieved

Method used

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  • A kind of preparation method of artificial cornea
  • A kind of preparation method of artificial cornea
  • A kind of preparation method of artificial cornea

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Material selection: collect eyeballs from pigs aged 4 to 12 months;

[0034] (2) Pretreatment: scrape off the corneal epithelial cells and corneal endothelial cells of the above-mentioned pig eyeballs, retain the Descemet's layer, prepare the lamellar cornea with a lamellar knife, and drill corneal slices of a specified size;

[0035] (3) Freezing and thawing: at normal temperature and pressure, place the corneal piece obtained in step (2) in liquid nitrogen for 10 to 30 minutes, take it out and slowly warm the corneal piece to room temperature at normal temperature and pressure, repeat for 5 to 10 minutes. Second-rate;

[0036] (4) Ultrasound: the corneal sheet after the freeze-thaw treatment in step (3) is placed in normal saline for ultrasonic cleaning under normal temperature and pressure, the ultrasonic working frequency is 20-200 kHz, and the ultrasonic time is 20-60 minutes;

[0037] (5) Enzyme degradation: under normal temperature and pressure, put the ultr...

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Abstract

The invention discloses a preparation method for artificial corneas. The preparation method comprises the following steps of (1) collecting age-appropriate pig eyeballs; (2) scraping corneal epithelial cells and corneal endothelial cells of the pig eyeballs, reserving bowman layers, preparing lamellar corneas by using a lamellar blade, and drilling size-specific corneal slices; (3) putting the corneal slices obtained in step (2) in liquid nitrogen for 8-35 minutes, slowly rewarming the corneal slices to room temperature under normal temperature and normal pressure after the corneal slices are taken out, repeating the operation for 4-12 times; (4) putting the corneal slices which are subjected to freezing-thawing treatment in step (3) in normal saline for carrying out ultrasonic cleaning, wherein the ultrasonic working frequency is 20-250 kHz, and the ultrasonic time is 15-65 minutes; (5) putting the corneal slices which are subjected to ultrasonic treatment in a buffer system, carrying out enzymolysis on DNA (Deoxyribose Nucleic Acid) of corneal stromal cells by using endonuclease, and obtaining the artificial corneas. According to the artificial corneas prepared by the preparation method disclosed by the invention, gaps between natural collagen fibers of the artificial corneas are beneficial for inducing the growth of the corneal cells and the growth of nerve fibers of an acceptor and promoting the repairing and the reconstructing of corneas of a donor.

Description

technical field [0001] The invention belongs to the technical field, in particular to a method for preparing an artificial cornea. Background technique [0002] The cornea is located at the front of the eyeball and is the first refractive medium through which light passes. Its transparency is the first element to ensure good visual function. Therefore, the artificial cornea is different from other tissue engineering products. After being prepared by different methods, it not only needs to maintain the good mechanical properties of its extracellular scaffold, but also has high requirements for the transparency of the scaffold material. [0003] With the development of tissue engineering technology in recent years, various "artificial organs" such as heterogeneous heart valves, skin and other heterogeneous organs have been successfully transplanted into human bodies. The study of artificial cornea brings new ideas and great enlightenment. As early as the beginning of this ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/14
Inventor 刘靖刘祖国李志寒李洁
Owner XIAMEN DAKAI BIOTECH CO LTD
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