Tissue culture rapid propagation method of lilium

A technology of tissue culture and lily, which is applied in the field of plant tissue culture, can solve the problems of strong gene specificity and lack of regeneration system, and achieve the effect of promoting the commercialization process

Inactive Publication Date: 2015-06-10
梁仕华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of methods for lily tissue culture have been successfully established, but due to the problem of strong gene specificity, there has been a lack of efficient and broad-spectrum regeneration systems. The present invention is simple, economical and easy to operate, and is easy to popularize and apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Induction of adventitious buds: select vigorous and plump lily bulbs, wash them with clean water, peel off the outer three layers of scales, take the middle and inner scales, soak them in 75% ethanol for 10 seconds, and rinse them with sterile water 5 times, 3 minutes each time, then sterilized with 0.1% mercuric chloride solution for 5 minutes, and then rinsed with sterilizing water 3 times, 3 minutes each time. The water droplets on the surface were blotted dry with sterile filter paper and inoculated on the induction medium for adventitious bud induction, and the induction rate reached 92.7%. The induction medium is 1 / 2MS+2mg / L 6-BA+0.1mg / L NAA+1mg / LGA 3 +3.5% sucrose+0.35% agar+0.1% activated carbon, the pH value is 5.8.

[0017] (2) Proliferation culture of adventitious buds: Inoculate the adventitious buds obtained in step (1) on the proliferation medium for proliferation culture. After inoculation, culture them in total darkness at 28°C for 7 days, and then ...

Embodiment 2

[0021] (1) Induction of adventitious buds: Select vigorous and plump lily bulbs, wash them with clean water, peel off the outer two layers of scales, take the middle and inner scales, soak them in 78% ethanol for 12 seconds, and rinse them with sterile water 5 times, 3 minutes each time, then sterilized with 0.15% mercuric chloride solution for 6 minutes, and then rinsed with sterilizing water 3 times, 3 minutes each time. Blot the water droplets on the surface with sterile filter paper and inoculate them on the induction medium to induce adventitious buds, and the induction rate reached 89.5.7%. The induction medium is 1 / 2MS+2.5mg / L 6-BA+0.6mg / L NAA+3mg / LGA 3 +3.5% sucrose+0.35% agar+0.1% activated carbon, the pH value is 5.8.

[0022] (2) Proliferation culture of adventitious buds: Inoculate the adventitious buds obtained in step (1) on the proliferation medium for proliferation culture. After inoculation, culture them in total darkness at 28°C for 9 days, and then place t...

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PUM

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Abstract

The invention discloses a tissue culture rapid propagation method of lilium and relates to a method of rapidly propagating lilium seedlings for keeping the original good traits of the parent from the lilium in short time by use of a tissue culture technique. According to the method, the tissue culture rapid propagation of the lilium is realized by taking the scales of L. Oriental hybrid Siberia as the explants and by virtue of the stages of adventitious bud induction, subculture, test-tube plantlet rooting, test-tube plantlet acclimatization and transplant and the like. As a result, the rapid propagation of the lilium seedlings can be realized under artificially controlled conditions; lots of test-tube plantlets can be propagated in short time and the commercial progress of the lilium can be promoted.

Description

technical field [0001] The invention relates to a plant tissue culture method in agricultural biotechnology, in particular to a lily tissue culture rapid propagation method. Background technique [0002] lily( Lilium ) is the general term for the perennial bulbous herbaceous plants of the monocot class Liliaceae Lilium. It is a traditional Chinese flower with high ornamental value and is deeply loved by the masses. There are about 100 species of Lilium, and there are more than 10,000 registered commercial lilies at present, which are mainly distributed in the cold and temperate mountainous areas of the northern hemisphere. In addition to its high ornamental value, it also has important edible and medicinal values. Its bulbs have anti-inflammatory, cough-relieving, and calming effects. [0003] Plant tissue culture has great application potential in rapid propagation, transgenic, detoxification, germplasm resource preservation and artificial seed production of lily. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 梁仕华
Owner 梁仕华
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