Tissue culture rapid propagation method of lilium
A technology of tissue culture and lily, which is applied in the field of plant tissue culture, can solve the problems of strong gene specificity and lack of regeneration system, and achieve the effect of promoting the commercialization process
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Embodiment 1
[0016] (1) Induction of adventitious buds: select vigorous and plump lily bulbs, wash them with clean water, peel off the outer three layers of scales, take the middle and inner scales, soak them in 75% ethanol for 10 seconds, and rinse them with sterile water 5 times, 3 minutes each time, then sterilized with 0.1% mercuric chloride solution for 5 minutes, and then rinsed with sterilizing water 3 times, 3 minutes each time. The water droplets on the surface were blotted dry with sterile filter paper and inoculated on the induction medium for adventitious bud induction, and the induction rate reached 92.7%. The induction medium is 1 / 2MS+2mg / L 6-BA+0.1mg / L NAA+1mg / LGA 3 +3.5% sucrose+0.35% agar+0.1% activated carbon, the pH value is 5.8.
[0017] (2) Proliferation culture of adventitious buds: Inoculate the adventitious buds obtained in step (1) on the proliferation medium for proliferation culture. After inoculation, culture them in total darkness at 28°C for 7 days, and then ...
Embodiment 2
[0021] (1) Induction of adventitious buds: Select vigorous and plump lily bulbs, wash them with clean water, peel off the outer two layers of scales, take the middle and inner scales, soak them in 78% ethanol for 12 seconds, and rinse them with sterile water 5 times, 3 minutes each time, then sterilized with 0.15% mercuric chloride solution for 6 minutes, and then rinsed with sterilizing water 3 times, 3 minutes each time. Blot the water droplets on the surface with sterile filter paper and inoculate them on the induction medium to induce adventitious buds, and the induction rate reached 89.5.7%. The induction medium is 1 / 2MS+2.5mg / L 6-BA+0.6mg / L NAA+3mg / LGA 3 +3.5% sucrose+0.35% agar+0.1% activated carbon, the pH value is 5.8.
[0022] (2) Proliferation culture of adventitious buds: Inoculate the adventitious buds obtained in step (1) on the proliferation medium for proliferation culture. After inoculation, culture them in total darkness at 28°C for 9 days, and then place t...
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